Bacillus phage BMBtp1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C8E969|A0A1C8E969_9CAUD Terminase small subunit OS=Bacillus phage BMBtp1 OX=1735561 GN=BMBtp1_1 PE=4 SV=1
MM1 pKa = 7.65NDD3 pKa = 4.34LIEE6 pKa = 4.41KK7 pKa = 9.75LKK9 pKa = 11.04SHH11 pKa = 6.04IHH13 pKa = 5.5WEE15 pKa = 3.97EE16 pKa = 4.11GMDD19 pKa = 4.38DD20 pKa = 3.95SLLSFYY26 pKa = 10.49IEE28 pKa = 3.88QAKK31 pKa = 9.53TYY33 pKa = 10.31VKK35 pKa = 10.64NATGKK40 pKa = 7.1QTEE43 pKa = 4.14YY44 pKa = 10.63LIIMVAGIYY53 pKa = 9.8YY54 pKa = 9.75DD55 pKa = 3.46YY56 pKa = 11.16RR57 pKa = 11.84VAEE60 pKa = 4.37KK61 pKa = 10.36EE62 pKa = 4.01LEE64 pKa = 4.13QALDD68 pKa = 3.38ALTPMFVQEE77 pKa = 4.21VYY79 pKa = 11.12ADD81 pKa = 3.86EE82 pKa = 4.92EE83 pKa = 4.3KK84 pKa = 11.3DD85 pKa = 3.52EE86 pKa = 4.21

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C8E992|A0A1C8E992_9CAUD Uncharacterized protein OS=Bacillus phage BMBtp1 OX=1735561 GN=BMBtp1_26 PE=4 SV=1
MM1 pKa = 7.18NHH3 pKa = 7.47LLTCSFNQKK12 pKa = 9.66IPIEE16 pKa = 4.29LIYY19 pKa = 11.01LNGSGDD25 pKa = 3.62FSQRR29 pKa = 11.84TVIVRR34 pKa = 11.84KK35 pKa = 9.7IYY37 pKa = 9.63EE38 pKa = 4.03DD39 pKa = 4.57RR40 pKa = 11.84ILVYY44 pKa = 10.46CMKK47 pKa = 9.7KK48 pKa = 9.08QQVRR52 pKa = 11.84TLKK55 pKa = 9.15FTNILSVDD63 pKa = 3.57KK64 pKa = 11.27VRR66 pKa = 11.84TKK68 pKa = 10.66YY69 pKa = 10.45QYY71 pKa = 11.46AA72 pKa = 3.51

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11051

37

1173

190.5

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.099 ± 0.518

0.805 ± 0.168

5.71 ± 0.246

8.008 ± 0.365

3.991 ± 0.149

5.8 ± 0.318

1.529 ± 0.174

7.447 ± 0.276

9.501 ± 0.402

7.782 ± 0.345

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.193

5.764 ± 0.246

2.95 ± 0.201

4.018 ± 0.296

4.343 ± 0.303

5.755 ± 0.257

5.71 ± 0.375

6.76 ± 0.323

1.366 ± 0.218

3.9 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski