Amino acid dipepetide frequency for Maribacter sp. 4U21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.953AlaAla: 4.953 ± 0.103
0.575AlaCys: 0.575 ± 0.026
3.795AlaAsp: 3.795 ± 0.079
4.142AlaGlu: 4.142 ± 0.066
3.335AlaPhe: 3.335 ± 0.06
4.37AlaGly: 4.37 ± 0.081
1.266AlaHis: 1.266 ± 0.034
5.549AlaIle: 5.549 ± 0.075
4.777AlaLys: 4.777 ± 0.078
6.953AlaLeu: 6.953 ± 0.101
1.752AlaMet: 1.752 ± 0.045
3.58AlaAsn: 3.58 ± 0.065
2.118AlaPro: 2.118 ± 0.05
2.586AlaGln: 2.586 ± 0.046
2.218AlaArg: 2.218 ± 0.05
4.313AlaSer: 4.313 ± 0.061
3.838AlaThr: 3.838 ± 0.061
4.511AlaVal: 4.511 ± 0.076
0.652AlaTrp: 0.652 ± 0.026
2.62AlaTyr: 2.62 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.508CysAla: 0.508 ± 0.023
0.109CysCys: 0.109 ± 0.01
0.376CysAsp: 0.376 ± 0.018
0.471CysGlu: 0.471 ± 0.024
0.371CysPhe: 0.371 ± 0.018
0.559CysGly: 0.559 ± 0.026
0.18CysHis: 0.18 ± 0.014
0.555CysIle: 0.555 ± 0.026
0.478CysLys: 0.478 ± 0.023
0.624CysLeu: 0.624 ± 0.025
0.128CysMet: 0.128 ± 0.011
0.379CysAsn: 0.379 ± 0.022
0.306CysPro: 0.306 ± 0.022
0.201CysGln: 0.201 ± 0.013
0.214CysArg: 0.214 ± 0.015
0.525CysSer: 0.525 ± 0.024
0.424CysThr: 0.424 ± 0.024
0.416CysVal: 0.416 ± 0.02
0.072CysTrp: 0.072 ± 0.009
0.287CysTyr: 0.287 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.08AspAla: 4.08 ± 0.087
0.389AspCys: 0.389 ± 0.02
3.093AspAsp: 3.093 ± 0.079
3.621AspGlu: 3.621 ± 0.062
3.905AspPhe: 3.905 ± 0.064
4.311AspGly: 4.311 ± 0.097
0.902AspHis: 0.902 ± 0.032
4.423AspIle: 4.423 ± 0.077
4.055AspLys: 4.055 ± 0.075
5.188AspLeu: 5.188 ± 0.063
1.263AspMet: 1.263 ± 0.037
3.085AspAsn: 3.085 ± 0.062
1.706AspPro: 1.706 ± 0.045
1.314AspGln: 1.314 ± 0.038
2.035AspArg: 2.035 ± 0.052
3.381AspSer: 3.381 ± 0.065
3.132AspThr: 3.132 ± 0.065
3.625AspVal: 3.625 ± 0.067
0.758AspTrp: 0.758 ± 0.029
2.846AspTyr: 2.846 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
4.491GluAla: 4.491 ± 0.071
0.329GluCys: 0.329 ± 0.017
3.731GluAsp: 3.731 ± 0.057
5.314GluGlu: 5.314 ± 0.076
2.858GluPhe: 2.858 ± 0.053
4.007GluGly: 4.007 ± 0.069
1.258GluHis: 1.258 ± 0.039
5.372GluIle: 5.372 ± 0.077
5.701GluLys: 5.701 ± 0.083
6.467GluLeu: 6.467 ± 0.089
1.662GluMet: 1.662 ± 0.047
4.363GluAsn: 4.363 ± 0.057
1.737GluPro: 1.737 ± 0.043
2.437GluGln: 2.437 ± 0.052
2.868GluArg: 2.868 ± 0.053
3.299GluSer: 3.299 ± 0.069
3.655GluThr: 3.655 ± 0.056
4.517GluVal: 4.517 ± 0.069
0.666GluTrp: 0.666 ± 0.027
2.239GluTyr: 2.239 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.041PheAla: 3.041 ± 0.063
0.419PheCys: 0.419 ± 0.02
3.214PheAsp: 3.214 ± 0.055
3.34PheGlu: 3.34 ± 0.066
2.784PhePhe: 2.784 ± 0.057
3.778PheGly: 3.778 ± 0.067
0.844PheHis: 0.844 ± 0.029
3.609PheIle: 3.609 ± 0.069
3.604PheLys: 3.604 ± 0.072
4.934PheLeu: 4.934 ± 0.074
1.194PheMet: 1.194 ± 0.034
3.001PheAsn: 3.001 ± 0.06
1.791PhePro: 1.791 ± 0.045
1.576PheGln: 1.576 ± 0.041
1.81PheArg: 1.81 ± 0.038
3.889PheSer: 3.889 ± 0.07
3.052PheThr: 3.052 ± 0.056
2.996PheVal: 2.996 ± 0.061
0.594PheTrp: 0.594 ± 0.026
2.16PheTyr: 2.16 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.673GlyAla: 4.673 ± 0.081
0.593GlyCys: 0.593 ± 0.034
3.741GlyAsp: 3.741 ± 0.066
3.878GlyGlu: 3.878 ± 0.07
3.776GlyPhe: 3.776 ± 0.063
4.914GlyGly: 4.914 ± 0.094
1.287GlyHis: 1.287 ± 0.038
5.774GlyIle: 5.774 ± 0.081
4.998GlyLys: 4.998 ± 0.07
6.39GlyLeu: 6.39 ± 0.084
1.723GlyMet: 1.723 ± 0.048
3.688GlyAsn: 3.688 ± 0.061
1.527GlyPro: 1.527 ± 0.047
1.984GlyGln: 1.984 ± 0.049
2.386GlyArg: 2.386 ± 0.057
4.124GlySer: 4.124 ± 0.071
4.311GlyThr: 4.311 ± 0.086
4.465GlyVal: 4.465 ± 0.065
0.8GlyTrp: 0.8 ± 0.034
2.825GlyTyr: 2.825 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.011HisAla: 1.011 ± 0.028
0.177HisCys: 0.177 ± 0.012
0.807HisAsp: 0.807 ± 0.028
0.988HisGlu: 0.988 ± 0.03
1.081HisPhe: 1.081 ± 0.035
1.097HisGly: 1.097 ± 0.029
0.475HisHis: 0.475 ± 0.023
1.423HisIle: 1.423 ± 0.035
1.214HisLys: 1.214 ± 0.036
1.825HisLeu: 1.825 ± 0.049
0.403HisMet: 0.403 ± 0.02
0.818HisAsn: 0.818 ± 0.028
0.942HisPro: 0.942 ± 0.035
0.718HisGln: 0.718 ± 0.027
0.753HisArg: 0.753 ± 0.027
1.034HisSer: 1.034 ± 0.033
0.991HisThr: 0.991 ± 0.03
1.016HisVal: 1.016 ± 0.035
0.246HisTrp: 0.246 ± 0.016
0.777HisTyr: 0.777 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.954IleAla: 5.954 ± 0.089
0.621IleCys: 0.621 ± 0.026
4.425IleAsp: 4.425 ± 0.063
4.695IleGlu: 4.695 ± 0.07
3.517IlePhe: 3.517 ± 0.077
5.117IleGly: 5.117 ± 0.089
1.233IleHis: 1.233 ± 0.032
5.261IleIle: 5.261 ± 0.095
5.132IleLys: 5.132 ± 0.076
6.934IleLeu: 6.934 ± 0.093
1.381IleMet: 1.381 ± 0.035
3.94IleAsn: 3.94 ± 0.068
3.186IlePro: 3.186 ± 0.058
2.364IleGln: 2.364 ± 0.051
2.735IleArg: 2.735 ± 0.052
5.292IleSer: 5.292 ± 0.076
4.658IleThr: 4.658 ± 0.067
4.685IleVal: 4.685 ± 0.072
0.716IleTrp: 0.716 ± 0.028
2.591IleTyr: 2.591 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
4.998LysAla: 4.998 ± 0.081
0.309LysCys: 0.309 ± 0.014
4.401LysAsp: 4.401 ± 0.077
6.403LysGlu: 6.403 ± 0.088
2.764LysPhe: 2.764 ± 0.049
4.79LysGly: 4.79 ± 0.072
1.249LysHis: 1.249 ± 0.038
5.534LysIle: 5.534 ± 0.084
6.648LysLys: 6.648 ± 0.103
6.325LysLeu: 6.325 ± 0.073
1.885LysMet: 1.885 ± 0.05
4.619LysAsn: 4.619 ± 0.075
2.231LysPro: 2.231 ± 0.047
2.349LysGln: 2.349 ± 0.046
3.123LysArg: 3.123 ± 0.056
4.455LysSer: 4.455 ± 0.067
4.35LysThr: 4.35 ± 0.063
4.532LysVal: 4.532 ± 0.068
0.814LysTrp: 0.814 ± 0.028
2.7LysTyr: 2.7 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
6.451LeuAla: 6.451 ± 0.111
0.658LeuCys: 0.658 ± 0.025
5.443LeuAsp: 5.443 ± 0.079
6.631LeuGlu: 6.631 ± 0.082
5.015LeuPhe: 5.015 ± 0.081
6.545LeuGly: 6.545 ± 0.102
1.589LeuHis: 1.589 ± 0.047
6.339LeuIle: 6.339 ± 0.098
7.594LeuLys: 7.594 ± 0.096
9.436LeuLeu: 9.436 ± 0.124
2.211LeuMet: 2.211 ± 0.046
5.47LeuAsn: 5.47 ± 0.085
3.684LeuPro: 3.684 ± 0.065
3.226LeuGln: 3.226 ± 0.06
3.696LeuArg: 3.696 ± 0.067
6.676LeuSer: 6.676 ± 0.075
4.999LeuThr: 4.999 ± 0.084
5.921LeuVal: 5.921 ± 0.074
0.922LeuTrp: 0.922 ± 0.031
3.126LeuTyr: 3.126 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.892MetAla: 1.892 ± 0.048
0.125MetCys: 0.125 ± 0.011
1.375MetAsp: 1.375 ± 0.037
1.653MetGlu: 1.653 ± 0.04
0.857MetPhe: 0.857 ± 0.032
1.638MetGly: 1.638 ± 0.047
0.422MetHis: 0.422 ± 0.02
1.363MetIle: 1.363 ± 0.041
2.01MetLys: 2.01 ± 0.042
2.058MetLeu: 2.058 ± 0.048
0.587MetMet: 0.587 ± 0.029
1.253MetAsn: 1.253 ± 0.039
0.9MetPro: 0.9 ± 0.03
0.834MetGln: 0.834 ± 0.029
0.946MetArg: 0.946 ± 0.034
1.416MetSer: 1.416 ± 0.044
1.203MetThr: 1.203 ± 0.033
1.587MetVal: 1.587 ± 0.044
0.181MetTrp: 0.181 ± 0.014
0.697MetTyr: 0.697 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.815AsnAla: 3.815 ± 0.057
0.421AsnCys: 0.421 ± 0.026
3.04AsnAsp: 3.04 ± 0.068
3.548AsnGlu: 3.548 ± 0.069
2.867AsnPhe: 2.867 ± 0.056
4.143AsnGly: 4.143 ± 0.09
0.974AsnHis: 0.974 ± 0.03
4.061AsnIle: 4.061 ± 0.068
3.675AsnLys: 3.675 ± 0.071
5.189AsnLeu: 5.189 ± 0.089
1.222AsnMet: 1.222 ± 0.035
3.125AsnAsn: 3.125 ± 0.064
2.678AsnPro: 2.678 ± 0.053
1.924AsnGln: 1.924 ± 0.05
2.27AsnArg: 2.27 ± 0.047
3.507AsnSer: 3.507 ± 0.061
3.603AsnThr: 3.603 ± 0.067
3.291AsnVal: 3.291 ± 0.054
0.724AsnTrp: 0.724 ± 0.025
2.545AsnTyr: 2.545 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.002ProAla: 2.002 ± 0.045
0.194ProCys: 0.194 ± 0.015
2.186ProAsp: 2.186 ± 0.055
2.995ProGlu: 2.995 ± 0.06
1.921ProPhe: 1.921 ± 0.05
2.058ProGly: 2.058 ± 0.044
0.635ProHis: 0.635 ± 0.027
2.611ProIle: 2.611 ± 0.05
2.673ProLys: 2.673 ± 0.053
3.137ProLeu: 3.137 ± 0.065
0.813ProMet: 0.813 ± 0.026
2.273ProAsn: 2.273 ± 0.05
0.895ProPro: 0.895 ± 0.033
1.082ProGln: 1.082 ± 0.033
1.036ProArg: 1.036 ± 0.032
2.174ProSer: 2.174 ± 0.046
1.993ProThr: 1.993 ± 0.048
2.39ProVal: 2.39 ± 0.047
0.392ProTrp: 0.392 ± 0.022
1.403ProTyr: 1.403 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.899GlnAla: 1.899 ± 0.045
0.164GlnCys: 0.164 ± 0.014
1.716GlnAsp: 1.716 ± 0.045
2.492GlnGlu: 2.492 ± 0.049
1.598GlnPhe: 1.598 ± 0.038
1.951GlnGly: 1.951 ± 0.047
0.644GlnHis: 0.644 ± 0.026
2.404GlnIle: 2.404 ± 0.047
2.658GlnLys: 2.658 ± 0.049
3.489GlnLeu: 3.489 ± 0.067
0.834GlnMet: 0.834 ± 0.028
1.979GlnAsn: 1.979 ± 0.044
1.065GlnPro: 1.065 ± 0.035
1.353GlnGln: 1.353 ± 0.037
1.43GlnArg: 1.43 ± 0.036
1.768GlnSer: 1.768 ± 0.044
1.682GlnThr: 1.682 ± 0.043
1.977GlnVal: 1.977 ± 0.043
0.406GlnTrp: 0.406 ± 0.018
1.224GlnTyr: 1.224 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.291ArgAla: 2.291 ± 0.05
0.21ArgCys: 0.21 ± 0.013
1.998ArgAsp: 1.998 ± 0.047
2.336ArgGlu: 2.336 ± 0.046
2.069ArgPhe: 2.069 ± 0.049
2.163ArgGly: 2.163 ± 0.052
0.634ArgHis: 0.634 ± 0.026
3.167ArgIle: 3.167 ± 0.054
3.111ArgLys: 3.111 ± 0.062
3.704ArgLeu: 3.704 ± 0.064
0.986ArgMet: 0.986 ± 0.03
2.254ArgAsn: 2.254 ± 0.054
1.308ArgPro: 1.308 ± 0.038
1.163ArgGln: 1.163 ± 0.031
1.488ArgArg: 1.488 ± 0.041
2.101ArgSer: 2.101 ± 0.047
2.179ArgThr: 2.179 ± 0.042
2.291ArgVal: 2.291 ± 0.05
0.399ArgTrp: 0.399 ± 0.019
1.689ArgTyr: 1.689 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
3.994SerAla: 3.994 ± 0.068
0.597SerCys: 0.597 ± 0.023
3.513SerAsp: 3.513 ± 0.065
4.069SerGlu: 4.069 ± 0.06
3.716SerPhe: 3.716 ± 0.063
4.681SerGly: 4.681 ± 0.077
1.042SerHis: 1.042 ± 0.034
4.697SerIle: 4.697 ± 0.076
4.76SerLys: 4.76 ± 0.071
6.164SerLeu: 6.164 ± 0.085
1.288SerMet: 1.288 ± 0.037
3.498SerAsn: 3.498 ± 0.071
2.07SerPro: 2.07 ± 0.049
1.953SerGln: 1.953 ± 0.05
2.24SerArg: 2.24 ± 0.049
4.062SerSer: 4.062 ± 0.079
3.384SerThr: 3.384 ± 0.061
3.936SerVal: 3.936 ± 0.058
0.715SerTrp: 0.715 ± 0.026
2.649SerTyr: 2.649 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.02ThrAla: 4.02 ± 0.057
0.358ThrCys: 0.358 ± 0.018
3.545ThrAsp: 3.545 ± 0.075
3.604ThrGlu: 3.604 ± 0.071
2.971ThrPhe: 2.971 ± 0.064
4.079ThrGly: 4.079 ± 0.072
0.953ThrHis: 0.953 ± 0.032
4.54ThrIle: 4.54 ± 0.073
3.68ThrLys: 3.68 ± 0.073
5.423ThrLeu: 5.423 ± 0.08
1.118ThrMet: 1.118 ± 0.033
3.083ThrAsn: 3.083 ± 0.078
2.425ThrPro: 2.425 ± 0.055
1.788ThrGln: 1.788 ± 0.037
1.787ThrArg: 1.787 ± 0.039
3.594ThrSer: 3.594 ± 0.058
3.486ThrThr: 3.486 ± 0.067
4.074ThrVal: 4.074 ± 0.076
0.564ThrTrp: 0.564 ± 0.031
2.357ThrTyr: 2.357 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.67ValAla: 4.67 ± 0.075
0.503ValCys: 0.503 ± 0.026
3.717ValAsp: 3.717 ± 0.07
3.634ValGlu: 3.634 ± 0.058
3.317ValPhe: 3.317 ± 0.059
4.078ValGly: 4.078 ± 0.067
1.178ValHis: 1.178 ± 0.033
4.586ValIle: 4.586 ± 0.073
4.176ValLys: 4.176 ± 0.068
6.577ValLeu: 6.577 ± 0.081
1.484ValMet: 1.484 ± 0.042
3.274ValAsn: 3.274 ± 0.063
2.479ValPro: 2.479 ± 0.045
1.991ValGln: 1.991 ± 0.048
2.308ValArg: 2.308 ± 0.053
4.506ValSer: 4.506 ± 0.068
3.701ValThr: 3.701 ± 0.078
4.512ValVal: 4.512 ± 0.078
0.642ValTrp: 0.642 ± 0.027
2.307ValTyr: 2.307 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.643TrpAla: 0.643 ± 0.023
0.092TrpCys: 0.092 ± 0.008
0.641TrpAsp: 0.641 ± 0.025
0.735TrpGlu: 0.735 ± 0.032
0.579TrpPhe: 0.579 ± 0.025
0.741TrpGly: 0.741 ± 0.029
0.246TrpHis: 0.246 ± 0.016
0.702TrpIle: 0.702 ± 0.029
0.828TrpLys: 0.828 ± 0.033
1.04TrpLeu: 1.04 ± 0.038
0.329TrpMet: 0.329 ± 0.018
0.7TrpAsn: 0.7 ± 0.024
0.314TrpPro: 0.314 ± 0.02
0.42TrpGln: 0.42 ± 0.021
0.441TrpArg: 0.441 ± 0.022
0.642TrpSer: 0.642 ± 0.024
0.528TrpThr: 0.528 ± 0.025
0.672TrpVal: 0.672 ± 0.025
0.163TrpTrp: 0.163 ± 0.013
0.455TrpTyr: 0.455 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.515TyrAla: 2.515 ± 0.05
0.338TyrCys: 0.338 ± 0.019
2.355TyrAsp: 2.355 ± 0.05
2.424TyrGlu: 2.424 ± 0.059
2.329TyrPhe: 2.329 ± 0.056
2.837TyrGly: 2.837 ± 0.054
0.786TyrHis: 0.786 ± 0.027
2.47TyrIle: 2.47 ± 0.055
2.705TyrLys: 2.705 ± 0.054
3.777TyrLeu: 3.777 ± 0.071
0.733TyrMet: 0.733 ± 0.029
2.237TyrAsn: 2.237 ± 0.054
1.499TyrPro: 1.499 ± 0.043
1.47TyrGln: 1.47 ± 0.042
1.759TyrArg: 1.759 ± 0.042
2.271TyrSer: 2.271 ± 0.053
2.278TyrThr: 2.278 ± 0.049
2.226TyrVal: 2.226 ± 0.046
0.486TyrTrp: 0.486 ± 0.024
1.712TyrTyr: 1.712 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3145 proteins (1053939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski