Microbacterium phage Zeta1847

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Zetavirus; Microbacterium virus Zeta1847

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QAQ2|A0A2Z4QAQ2_9CAUD Uncharacterized protein OS=Microbacterium phage Zeta1847 OX=2201444 GN=49 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 5.78AAPADD7 pKa = 3.82FTDD10 pKa = 3.99PAEE13 pKa = 4.36LADD16 pKa = 4.24HH17 pKa = 7.23RR18 pKa = 11.84EE19 pKa = 4.06ACDD22 pKa = 3.41LCEE25 pKa = 4.1ACDD28 pKa = 3.6PFRR31 pKa = 11.84AFGACSTCHH40 pKa = 7.16DD41 pKa = 4.33DD42 pKa = 5.67AEE44 pKa = 5.61AIGPAEE50 pKa = 4.76LSPCCGSPVYY60 pKa = 10.43FPGPVEE66 pKa = 4.74DD67 pKa = 4.25GPEE70 pKa = 3.66RR71 pKa = 11.84FACFTT76 pKa = 4.0

Molecular weight: 8.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q9E5|A0A2Z4Q9E5_9CAUD Uncharacterized protein OS=Microbacterium phage Zeta1847 OX=2201444 GN=62 PE=4 SV=1
MM1 pKa = 7.81TDD3 pKa = 2.98TTSALSIEE11 pKa = 4.2QRR13 pKa = 11.84RR14 pKa = 11.84DD15 pKa = 2.99ILVRR19 pKa = 11.84EE20 pKa = 4.16LKK22 pKa = 10.31KK23 pKa = 11.05YY24 pKa = 10.56RR25 pKa = 11.84GDD27 pKa = 3.57GYY29 pKa = 11.34AVDD32 pKa = 4.17VSPDD36 pKa = 3.25GLTATLTRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.84RR47 pKa = 11.84INPLVFVILAIFTLGIALIWFAFRR71 pKa = 11.84AANRR75 pKa = 11.84KK76 pKa = 7.05TEE78 pKa = 4.13TVILRR83 pKa = 11.84VDD85 pKa = 3.38EE86 pKa = 4.28RR87 pKa = 11.84GKK89 pKa = 10.49IIRR92 pKa = 11.84AA93 pKa = 3.52

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14742

37

870

196.6

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.876 ± 0.407

0.705 ± 0.13

6.329 ± 0.219

6.641 ± 0.335

2.503 ± 0.179

7.821 ± 0.404

2.116 ± 0.156

3.663 ± 0.281

3.08 ± 0.224

9.463 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.635 ± 0.136

2.049 ± 0.171

5.84 ± 0.289

1.621 ± 0.188

8.459 ± 0.444

5.216 ± 0.252

6.994 ± 0.315

7.265 ± 0.221

1.547 ± 0.151

2.177 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski