Thalassoglobus neptunius
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5460 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5X3V5|A0A5C5X3V5_9PLAN Uncharacterized protein OS=Thalassoglobus neptunius OX=1938619 GN=KOR42_03580 PE=4 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 FAFVFAVLCLTGSFVQAQTTLYY25 pKa = 10.61 SDD27 pKa = 3.98 SFTNDD32 pKa = 2.28 GSAVNLPSWDD42 pKa = 3.52 TVEE45 pKa = 5.49 NGGVWWNDD53 pKa = 3.1 GSDD56 pKa = 3.54 STPWVTWSGTDD67 pKa = 3.48 SVAHH71 pKa = 6.51 HH72 pKa = 6.67 SLDD75 pKa = 4.27 AIVPGSEE82 pKa = 3.79 HH83 pKa = 7.35 SGIDD87 pKa = 3.37 FGAADD92 pKa = 3.67 VDD94 pKa = 3.58 ITFEE98 pKa = 4.21 FNYY101 pKa = 9.12 GTGTTASYY109 pKa = 11.51 DD110 pKa = 3.69 SIFWIRR116 pKa = 11.84 ANSDD120 pKa = 2.77 RR121 pKa = 11.84 SEE123 pKa = 3.99 ALGIYY128 pKa = 10.03 HH129 pKa = 6.54 SVVDD133 pKa = 5.33 DD134 pKa = 4.03 EE135 pKa = 4.68 FTLVDD140 pKa = 4.12 EE141 pKa = 5.38 DD142 pKa = 4.36 EE143 pKa = 4.56 VDD145 pKa = 3.8 LGEE148 pKa = 4.34 TPVASGLTTANTYY161 pKa = 7.02 YY162 pKa = 10.38 TIRR165 pKa = 11.84 ILLSGSKK172 pKa = 9.94 ISLWVDD178 pKa = 3.18 DD179 pKa = 4.15 VLKK182 pKa = 10.41 IDD184 pKa = 3.44 EE185 pKa = 4.88 WDD187 pKa = 3.29 NSTYY191 pKa = 8.59 QTNTYY196 pKa = 10.17 FGFHH200 pKa = 7.3 AISGGRR206 pKa = 11.84 LDD208 pKa = 5.07 DD209 pKa = 4.5 FSVTDD214 pKa = 3.71 VVTSGISIEE223 pKa = 4.03 VHH225 pKa = 5.66 GDD227 pKa = 3.49 EE228 pKa = 5.07 IVDD231 pKa = 4.37 AGTEE235 pKa = 4.19 DD236 pKa = 3.67 TGSVPQGSWVTVPIEE251 pKa = 4.09 MIGVSASTVTVNNFAVSGDD270 pKa = 3.94 LLFDD274 pKa = 5.09 DD275 pKa = 5.46 SDD277 pKa = 4.25 DD278 pKa = 4.11 FSSFTLDD285 pKa = 3.02 QDD287 pKa = 3.57 EE288 pKa = 4.59 TNKK291 pKa = 9.67 IRR293 pKa = 11.84 VRR295 pKa = 11.84 LSAATAGSKK304 pKa = 9.26 TGTVTVTYY312 pKa = 8.23 NTTEE316 pKa = 3.89 NYY318 pKa = 8.41 TINVDD323 pKa = 3.19 WTVVAKK329 pKa = 10.01 TPASDD334 pKa = 3.3 VALTKK339 pKa = 10.79 GGGGSAPTDD348 pKa = 3.61 PTRR351 pKa = 11.84 TSYY354 pKa = 11.22 KK355 pKa = 9.88 PGGMWTQVPFAQTVWADD372 pKa = 3.23 EE373 pKa = 4.07 TTYY376 pKa = 10.68 IGVIAMQEE384 pKa = 4.0 GGIEE388 pKa = 5.07 SVDD391 pKa = 3.99 FWWNGNSVTVNTMTTHH407 pKa = 7.19 DD408 pKa = 5.22 GISAYY413 pKa = 10.59 YY414 pKa = 9.92 CAISPNAASGNEE426 pKa = 4.05 QIYY429 pKa = 8.52 ATINAADD436 pKa = 4.75 PYY438 pKa = 10.16 AQEE441 pKa = 4.83 KK442 pKa = 10.5 VLDD445 pKa = 3.99 DD446 pKa = 3.93 PFYY449 pKa = 10.59 FTIFDD454 pKa = 3.73 SATVNEE460 pKa = 4.34 VHH462 pKa = 6.45 VGRR465 pKa = 11.84 GDD467 pKa = 3.33 ASAGSGTEE475 pKa = 3.69 GDD477 pKa = 4.66 PYY479 pKa = 11.38 LSLQSAINDD488 pKa = 3.43 LSLTDD493 pKa = 3.82 GGIVTIHH500 pKa = 6.94 EE501 pKa = 4.61 GTVDD505 pKa = 3.25 WPYY508 pKa = 10.49 TATPKK513 pKa = 10.65 ANSGVFWLRR522 pKa = 11.84 MAEE525 pKa = 4.38 GEE527 pKa = 4.2 DD528 pKa = 3.57 RR529 pKa = 11.84 YY530 pKa = 11.33 NVVKK534 pKa = 10.84 NFTGTASTSIRR545 pKa = 11.84 LSVEE549 pKa = 3.63 RR550 pKa = 11.84 LKK552 pKa = 11.39 VSGIEE557 pKa = 3.73 WDD559 pKa = 3.72 TANAAVFYY567 pKa = 10.55 NRR569 pKa = 11.84 GVSGYY574 pKa = 7.77 YY575 pKa = 8.47 TVWDD579 pKa = 5.39 DD580 pKa = 3.28 IHH582 pKa = 8.25 VGNSDD587 pKa = 3.73 GRR589 pKa = 11.84 FTLVNGNHH597 pKa = 6.81 LNTGSIGRR605 pKa = 11.84 AAGTTDD611 pKa = 3.35 KK612 pKa = 11.52 DD613 pKa = 3.54 GFQNCRR619 pKa = 11.84 FVDD622 pKa = 4.67 TYY624 pKa = 11.3 QGTTGFYY631 pKa = 10.49 SINDD635 pKa = 3.28 YY636 pKa = 9.98 VEE638 pKa = 4.29 RR639 pKa = 11.84 VLRR642 pKa = 11.84 DD643 pKa = 3.49 HH644 pKa = 5.66 STCWSVANMVSRR656 pKa = 11.84 RR657 pKa = 11.84 GDD659 pKa = 3.27 YY660 pKa = 10.71 DD661 pKa = 3.61 YY662 pKa = 11.75 LRR664 pKa = 11.84 DD665 pKa = 3.73 NLAALTITYY674 pKa = 10.23 SGGATATYY682 pKa = 11.15 NLTSTNAGSSRR693 pKa = 11.84 DD694 pKa = 3.7 LVLLEE699 pKa = 4.81 DD700 pKa = 4.72 ASPVLTINLKK710 pKa = 8.23 TSSSAVVDD718 pKa = 3.53 TDD720 pKa = 4.46 DD721 pKa = 5.19 ADD723 pKa = 4.31 GEE725 pKa = 4.34 IDD727 pKa = 3.24 AHH729 pKa = 6.42 TIAQVVSAINATTDD743 pKa = 2.62 WSASIDD749 pKa = 3.51 TDD751 pKa = 3.87 YY752 pKa = 11.56 TSAAADD758 pKa = 3.9 RR759 pKa = 11.84 QLDD762 pKa = 3.71 ATYY765 pKa = 10.97 LKK767 pKa = 10.21 IDD769 pKa = 3.36 AHH771 pKa = 6.45 FSAVTGGAIPVDD783 pKa = 4.64 LYY785 pKa = 11.36 VAADD789 pKa = 3.47 LHH791 pKa = 7.37 VDD793 pKa = 5.27 GIQSSYY799 pKa = 8.78 GTGVAYY805 pKa = 10.77 NRR807 pKa = 11.84 FYY809 pKa = 11.45 YY810 pKa = 10.82 NCDD813 pKa = 3.26 FDD815 pKa = 5.84 NNPADD820 pKa = 3.63 MVATYY825 pKa = 10.89 GNNEE829 pKa = 3.97 SDD831 pKa = 3.54 MQGLFIVGTNGGHH844 pKa = 6.16 TDD846 pKa = 3.07 GGAYY850 pKa = 9.83 FMHH853 pKa = 6.63 YY854 pKa = 10.68 KK855 pKa = 10.49 NVDD858 pKa = 3.18 ADD860 pKa = 3.72 NASQFIGEE868 pKa = 4.06 MDD870 pKa = 4.1 NFNMMFCTFEE880 pKa = 4.47 GPQIMSPRR888 pKa = 11.84 TDD890 pKa = 2.65 KK891 pKa = 11.37 VGNQEE896 pKa = 4.41 YY897 pKa = 10.27 IPTNVVFRR905 pKa = 11.84 GNAFLHH911 pKa = 6.27 LDD913 pKa = 3.06 NWEE916 pKa = 4.1 DD917 pKa = 3.57 QFGGEE922 pKa = 5.25 LYY924 pKa = 10.37 FANNSQVTDD933 pKa = 3.29 DD934 pKa = 3.91 WSAIGYY940 pKa = 10.38 SNTQSANVAAWDD952 pKa = 4.22 LNTDD956 pKa = 3.54 GTVGDD961 pKa = 4.47 SSTLLDD967 pKa = 3.56 RR968 pKa = 11.84 SLNGTAYY975 pKa = 8.11 QTLWPNGDD983 pKa = 3.28 SMASDD988 pKa = 3.84 GSDD991 pKa = 4.67 PIGAWPPEE999 pKa = 4.42 GGVTPTPNGDD1009 pKa = 3.69 DD1010 pKa = 4.11 DD1011 pKa = 5.45 GEE1013 pKa = 4.19 EE1014 pKa = 4.08 SLLLRR1019 pKa = 11.84 FAQRR1023 pKa = 11.84 TWRR1026 pKa = 11.84 PQWRR1030 pKa = 11.84 RR1031 pKa = 11.84 DD1032 pKa = 3.32 MSLLRR1037 pKa = 11.84 SQQQ1040 pKa = 2.93
Molecular weight: 112.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.923
IPC_protein 3.973
Toseland 3.745
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.795
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.075
DTASelect 4.368
Thurlkill 3.795
EMBOSS 3.923
Sillero 4.101
Patrickios 2.206
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 3.988
Protein with the highest isoelectric point:
>tr|A0A5C5WPP0|A0A5C5WPP0_9PLAN Xylose isomerase-like TIM barrel OS=Thalassoglobus neptunius OX=1938619 GN=KOR42_30930 PE=4 SV=1
MM1 pKa = 7.27 PRR3 pKa = 11.84 KK4 pKa = 9.56 RR5 pKa = 11.84 SQVTIPEE12 pKa = 4.05 EE13 pKa = 3.82 LATTFATSEE22 pKa = 4.09 RR23 pKa = 11.84 CPSRR27 pKa = 11.84 EE28 pKa = 3.88 SVRR31 pKa = 11.84 TIDD34 pKa = 3.98 LTGTLKK40 pKa = 10.21 TIRR43 pKa = 11.84 SRR45 pKa = 11.84 VRR47 pKa = 11.84 EE48 pKa = 4.13 NCPQSPGVYY57 pKa = 10.24 SLINSEE63 pKa = 3.99 NQIVYY68 pKa = 10.55 VGMSVSLANRR78 pKa = 11.84 LQTYY82 pKa = 7.48 FTQKK86 pKa = 8.63 RR87 pKa = 11.84 ARR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 EE92 pKa = 3.8 SRR94 pKa = 11.84 IRR96 pKa = 11.84 RR97 pKa = 11.84 AAVRR101 pKa = 11.84 LIWQPLDD108 pKa = 3.16 HH109 pKa = 6.8 GLIARR114 pKa = 11.84 IRR116 pKa = 11.84 EE117 pKa = 3.85 RR118 pKa = 11.84 EE119 pKa = 4.35 LIRR122 pKa = 11.84 KK123 pKa = 7.68 FRR125 pKa = 11.84 PLMNVIGHH133 pKa = 5.84 PTQLKK138 pKa = 7.39 TGYY141 pKa = 9.64 VVAIDD146 pKa = 3.93 HH147 pKa = 6.49 PVGGFEE153 pKa = 4.58 FVEE156 pKa = 4.42 EE157 pKa = 4.15 LRR159 pKa = 11.84 GRR161 pKa = 11.84 YY162 pKa = 8.73 QGIWGPIPFNQWSRR176 pKa = 11.84 DD177 pKa = 3.36 ATEE180 pKa = 3.85 EE181 pKa = 4.41 LNLHH185 pKa = 5.88 FQLRR189 pKa = 11.84 DD190 pKa = 3.71 CPKK193 pKa = 9.06 STVLYY198 pKa = 10.11 FSGQPTPEE206 pKa = 4.44 APSTPCLRR214 pKa = 11.84 ADD216 pKa = 4.84 LGTCLSPCIGKK227 pKa = 10.02 CSKK230 pKa = 9.24 TAYY233 pKa = 10.02 LRR235 pKa = 11.84 AFKK238 pKa = 10.47 RR239 pKa = 11.84 ARR241 pKa = 11.84 SFLNGSAEE249 pKa = 4.08 DD250 pKa = 3.54 VTAEE254 pKa = 4.31 IEE256 pKa = 4.31 TQMRR260 pKa = 11.84 EE261 pKa = 3.8 AAGEE265 pKa = 4.3 KK266 pKa = 10.36 NFEE269 pKa = 3.75 TAARR273 pKa = 11.84 LRR275 pKa = 11.84 DD276 pKa = 3.31 RR277 pKa = 11.84 MKK279 pKa = 10.92 AFRR282 pKa = 11.84 YY283 pKa = 10.45 LNDD286 pKa = 3.47 RR287 pKa = 11.84 LRR289 pKa = 11.84 RR290 pKa = 11.84 FHH292 pKa = 7.51 DD293 pKa = 2.99 WTNRR297 pKa = 11.84 ANFVYY302 pKa = 10.38 RR303 pKa = 11.84 FTSEE307 pKa = 3.88 IDD309 pKa = 3.45 SQPRR313 pKa = 11.84 WLVVSRR319 pKa = 11.84 GVIHH323 pKa = 6.51 QLIDD327 pKa = 3.53 EE328 pKa = 4.74 PSSVEE333 pKa = 3.67 QAMGILNPLNSSRR346 pKa = 11.84 TRR348 pKa = 11.84 HH349 pKa = 5.64 SKK351 pKa = 7.97 PTRR354 pKa = 11.84 PNEE357 pKa = 3.75 QLRR360 pKa = 11.84 PGDD363 pKa = 3.88 FEE365 pKa = 3.94 TARR368 pKa = 11.84 ILFRR372 pKa = 11.84 WFRR375 pKa = 11.84 QFPKK379 pKa = 10.27 EE380 pKa = 3.75 KK381 pKa = 9.57 TSRR384 pKa = 11.84 LSYY387 pKa = 10.38 SAALRR392 pKa = 11.84 LIKK395 pKa = 10.07 RR396 pKa = 11.84 RR397 pKa = 11.84 RR398 pKa = 11.84 ADD400 pKa = 2.96
Molecular weight: 46.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.575
IPC_protein 10.511
Toseland 10.613
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 10.804
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.613
DTASelect 10.467
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.467
IPC_peptide 10.862
IPC2_peptide 9.633
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5460
0
5460
1904778
29
3664
348.9
38.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.109 ± 0.029
1.173 ± 0.012
6.041 ± 0.028
7.109 ± 0.033
4.068 ± 0.019
7.316 ± 0.038
2.235 ± 0.016
5.39 ± 0.021
3.8 ± 0.029
9.672 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.152 ± 0.014
3.508 ± 0.025
5.069 ± 0.021
4.205 ± 0.022
6.396 ± 0.028
7.192 ± 0.032
5.529 ± 0.026
7.15 ± 0.027
1.509 ± 0.013
2.377 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here