Cassava vein mosaic virus (CsVMV)
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q66284|IBMP_CSVMV Putative transactivator/viroplasmin protein OS=Cassava vein mosaic virus OX=38062 GN=ORF 4 PE=3 SV=1
MM1 pKa = 8.0 DD2 pKa = 5.4 SKK4 pKa = 11.67 DD5 pKa = 3.52 FTQLNLEE12 pKa = 4.03 EE13 pKa = 4.68 HH14 pKa = 6.26 SYY16 pKa = 10.51 KK17 pKa = 10.57 VNRR20 pKa = 11.84 EE21 pKa = 3.75 KK22 pKa = 11.23 LPIDD26 pKa = 4.01 SYY28 pKa = 10.55 IQYY31 pKa = 11.16 GGFTYY36 pKa = 11.08 ANFTPYY42 pKa = 9.93 IIHH45 pKa = 7.1 GDD47 pKa = 3.72 DD48 pKa = 3.58 GFGEE52 pKa = 4.33 HH53 pKa = 6.9 KK54 pKa = 10.19 QLNWTNKK61 pKa = 10.14 LLWNKK66 pKa = 9.84 LGKK69 pKa = 10.6 LNIKK73 pKa = 8.6 DD74 pKa = 3.91 TQILMQNNISEE85 pKa = 4.35 EE86 pKa = 3.92 QHH88 pKa = 6.18 NEE90 pKa = 4.18 LISLEE95 pKa = 4.05 AQKK98 pKa = 10.38 IARR101 pKa = 11.84 EE102 pKa = 3.88 NLADD106 pKa = 5.82 RR107 pKa = 11.84 INYY110 pKa = 7.52 LQNINTSIDD119 pKa = 3.41 FKK121 pKa = 10.33 LWKK124 pKa = 9.16 MNKK127 pKa = 9.35 EE128 pKa = 3.71 NLEE131 pKa = 4.04 RR132 pKa = 11.84 QEE134 pKa = 4.16 LLLRR138 pKa = 11.84 QINEE142 pKa = 3.93 LKK144 pKa = 10.98 EE145 pKa = 4.17 EE146 pKa = 4.08 IKK148 pKa = 10.63 SLKK151 pKa = 10.22 NIPSTVAIIPTNTYY165 pKa = 7.99 TINMIRR171 pKa = 11.84 TEE173 pKa = 4.19 TEE175 pKa = 3.07 DD176 pKa = 2.92 WKK178 pKa = 10.29 YY179 pKa = 10.88 FKK181 pKa = 10.89 YY182 pKa = 10.16 IEE184 pKa = 4.65 KK185 pKa = 10.56 EE186 pKa = 3.78 LVQNKK191 pKa = 8.4 TEE193 pKa = 4.8 AIAKK197 pKa = 9.82 ILDD200 pKa = 3.23 NSYY203 pKa = 10.65 IINDD207 pKa = 3.75 NLGLLYY213 pKa = 10.43 EE214 pKa = 4.73 RR215 pKa = 11.84 YY216 pKa = 10.1 EE217 pKa = 4.55 EE218 pKa = 4.43 INPTPKK224 pKa = 9.92 PYY226 pKa = 10.19 KK227 pKa = 9.83 RR228 pKa = 11.84 PEE230 pKa = 4.31 TIFDD234 pKa = 3.53 TPQYY238 pKa = 11.06 AKK240 pKa = 10.72 YY241 pKa = 10.15 IRR243 pKa = 11.84 NQKK246 pKa = 9.16 RR247 pKa = 11.84 QEE249 pKa = 4.1 EE250 pKa = 4.62 YY251 pKa = 10.83 EE252 pKa = 4.09 KK253 pKa = 10.66 QQEE256 pKa = 4.12 LKK258 pKa = 10.73 KK259 pKa = 10.44 EE260 pKa = 4.17 NEE262 pKa = 3.82 NKK264 pKa = 10.14 EE265 pKa = 4.04 YY266 pKa = 10.99 QEE268 pKa = 3.77 FLEE271 pKa = 4.47 WKK273 pKa = 9.86 EE274 pKa = 3.91 KK275 pKa = 7.27 QQKK278 pKa = 10.48 DD279 pKa = 3.39 KK280 pKa = 11.57 GKK282 pKa = 10.63 GIQTVYY288 pKa = 8.34 PTLIIPDD295 pKa = 4.13 IKK297 pKa = 10.67 PEE299 pKa = 4.04 KK300 pKa = 9.43 QKK302 pKa = 11.13 KK303 pKa = 8.1 EE304 pKa = 3.92 DD305 pKa = 3.7 MMLEE309 pKa = 4.18 MIKK312 pKa = 10.42 NLQNEE317 pKa = 4.6 LEE319 pKa = 3.95 QLKK322 pKa = 9.62 IQRR325 pKa = 11.84 HH326 pKa = 5.13 KK327 pKa = 10.42 EE328 pKa = 3.82 HH329 pKa = 6.96 EE330 pKa = 4.29 KK331 pKa = 10.2 QAEE334 pKa = 4.12 LTKK337 pKa = 10.41 IQMLEE342 pKa = 3.99 EE343 pKa = 4.05 EE344 pKa = 5.04 LEE346 pKa = 4.28 EE347 pKa = 5.24 EE348 pKa = 4.56 LDD350 pKa = 3.71 PDD352 pKa = 3.82 NLEE355 pKa = 4.61 KK356 pKa = 10.76 EE357 pKa = 4.5 VLNNIQNIQISSDD370 pKa = 2.79 ISEE373 pKa = 3.88 SSEE376 pKa = 3.54 INEE379 pKa = 3.85 ISDD382 pKa = 3.79 NEE384 pKa = 4.28 TEE386 pKa = 4.38 QISGSDD392 pKa = 2.76 SDD394 pKa = 4.54 YY395 pKa = 11.55 NNEE398 pKa = 3.96 QINVKK403 pKa = 10.06 IEE405 pKa = 3.95 GEE407 pKa = 3.99 EE408 pKa = 3.91 YY409 pKa = 10.16 EE410 pKa = 4.54 YY411 pKa = 11.24 KK412 pKa = 10.8 DD413 pKa = 2.89 NYY415 pKa = 10.44 RR416 pKa = 11.84 YY417 pKa = 10.1 YY418 pKa = 10.69 KK419 pKa = 9.43 PQPPYY424 pKa = 8.89 YY425 pKa = 9.84 KK426 pKa = 10.03 KK427 pKa = 10.41 DD428 pKa = 3.07 IRR430 pKa = 11.84 RR431 pKa = 11.84 EE432 pKa = 3.8 RR433 pKa = 11.84 QYY435 pKa = 11.22 KK436 pKa = 8.57 GQSSQRR442 pKa = 11.84 ADD444 pKa = 3.44 YY445 pKa = 10.5 IKK447 pKa = 10.61 NRR449 pKa = 11.84 RR450 pKa = 11.84 EE451 pKa = 4.05 QFEE454 pKa = 4.3 STYY457 pKa = 9.21 QANMNTTINDD467 pKa = 3.23 SGEE470 pKa = 4.08 ILNLDD475 pKa = 3.48 CTTPEE480 pKa = 3.86 EE481 pKa = 4.73 AEE483 pKa = 4.53 DD484 pKa = 5.52 RR485 pKa = 11.84 IQKK488 pKa = 6.85 WTQSMSIALVKK499 pKa = 10.31 QQLSNEE505 pKa = 4.0 QAKK508 pKa = 9.86 QFIRR512 pKa = 11.84 RR513 pKa = 11.84 TFIGNVKK520 pKa = 9.17 EE521 pKa = 4.18 WYY523 pKa = 10.1 KK524 pKa = 11.34 NLTNEE529 pKa = 4.59 AKK531 pKa = 10.29 QKK533 pKa = 11.15 LEE535 pKa = 3.95 GNAPLLSLTHH545 pKa = 6.13 MEE547 pKa = 4.19 LGLRR551 pKa = 11.84 AEE553 pKa = 4.31 FGKK556 pKa = 10.84 LGIEE560 pKa = 4.16 SDD562 pKa = 4.0 VEE564 pKa = 3.96 KK565 pKa = 10.86 HH566 pKa = 5.35 EE567 pKa = 4.61 KK568 pKa = 7.8 KK569 pKa = 9.44 TSIARR574 pKa = 11.84 HH575 pKa = 6.34 KK576 pKa = 10.31 ILQLQICSMDD586 pKa = 3.53 HH587 pKa = 6.0 QNLNAYY593 pKa = 9.55 LCEE596 pKa = 3.94 FQEE599 pKa = 4.71 YY600 pKa = 9.5 YY601 pKa = 11.06 YY602 pKa = 10.74 SANYY606 pKa = 8.88 TEE608 pKa = 5.48 AEE610 pKa = 4.22 SEE612 pKa = 4.24 NILNMFYY619 pKa = 11.07 SKK621 pKa = 10.77 LPEE624 pKa = 3.94 PWGQQVLNGYY634 pKa = 9.97 LSEE637 pKa = 4.24 IKK639 pKa = 10.52 GKK641 pKa = 9.99 NLLDD645 pKa = 4.18 SIGARR650 pKa = 11.84 MTYY653 pKa = 9.74 LQEE656 pKa = 4.86 FISDD660 pKa = 3.49 KK661 pKa = 11.14 CKK663 pKa = 10.88 EE664 pKa = 3.83 NWTQKK669 pKa = 7.57 QARR672 pKa = 11.84 KK673 pKa = 9.02 IQLSKK678 pKa = 11.25 NLDD681 pKa = 3.19 CSYY684 pKa = 11.45 YY685 pKa = 10.15 EE686 pKa = 3.95 VGKK689 pKa = 10.44 YY690 pKa = 9.46 GCKK693 pKa = 9.8 QIRR696 pKa = 11.84 PHH698 pKa = 5.92 KK699 pKa = 9.75 RR700 pKa = 11.84 KK701 pKa = 9.78 RR702 pKa = 11.84 YY703 pKa = 7.1 YY704 pKa = 10.49 KK705 pKa = 10.15 KK706 pKa = 10.49 YY707 pKa = 9.82 IPIKK711 pKa = 10.06 RR712 pKa = 11.84 KK713 pKa = 8.95 YY714 pKa = 9.73 FNKK717 pKa = 9.67 KK718 pKa = 9.26 RR719 pKa = 11.84 YY720 pKa = 8.41 KK721 pKa = 10.14 KK722 pKa = 10.06 YY723 pKa = 9.82 YY724 pKa = 9.32 RR725 pKa = 11.84 PKK727 pKa = 10.68 KK728 pKa = 9.28 FLKK731 pKa = 10.27 RR732 pKa = 11.84 KK733 pKa = 9.44 NPHH736 pKa = 6.39 KK737 pKa = 10.71 ACKK740 pKa = 9.94 CYY742 pKa = 9.85 NCGEE746 pKa = 4.34 EE747 pKa = 4.31 GHH749 pKa = 6.84 ISPNCKK755 pKa = 9.78 KK756 pKa = 9.79 PKK758 pKa = 9.8 KK759 pKa = 8.8 KK760 pKa = 8.88 TRR762 pKa = 11.84 INNLEE767 pKa = 3.73 ALEE770 pKa = 4.19 FKK772 pKa = 9.47 NTEE775 pKa = 3.87 MEE777 pKa = 4.09 NLEE780 pKa = 4.4 FEE782 pKa = 4.56 TNKK785 pKa = 10.26 NDD787 pKa = 4.09 IIWVEE792 pKa = 4.44 EE793 pKa = 4.03 IEE795 pKa = 4.79 VIQPLHH801 pKa = 6.99 YY802 pKa = 10.68 EE803 pKa = 3.96 EE804 pKa = 4.37 EE805 pKa = 4.21 EE806 pKa = 4.39 KK807 pKa = 11.43 YY808 pKa = 10.47 KK809 pKa = 11.17 GNYY812 pKa = 8.39 SDD814 pKa = 6.28 RR815 pKa = 11.84 ILQNPYY821 pKa = 9.37 YY822 pKa = 9.42 INSISIEE829 pKa = 4.07 EE830 pKa = 4.51 LDD832 pKa = 3.61 NLDD835 pKa = 3.86 WEE837 pKa = 4.76 FEE839 pKa = 4.19 YY840 pKa = 11.26 QEE842 pKa = 5.66 DD843 pKa = 4.44 IEE845 pKa = 6.84 DD846 pKa = 3.93 DD847 pKa = 4.04 LEE849 pKa = 4.34 YY850 pKa = 11.14 QNFVYY855 pKa = 10.59 QEE857 pKa = 4.53 SNDD860 pKa = 3.18 NWYY863 pKa = 10.81 SDD865 pKa = 3.51 QEE867 pKa = 3.8 NWYY870 pKa = 10.7 SDD872 pKa = 3.39 EE873 pKa = 4.23 QYY875 pKa = 11.15 LGIYY879 pKa = 8.2 MFIGEE884 pKa = 4.43 TSGEE888 pKa = 4.01 NNQDD892 pKa = 3.15 NMEE895 pKa = 4.71 GIIKK899 pKa = 9.6 EE900 pKa = 4.18 YY901 pKa = 11.14 NKK903 pKa = 10.05 TEE905 pKa = 3.85 PEE907 pKa = 4.51 KK908 pKa = 10.51 INKK911 pKa = 9.56 IIFTSEE917 pKa = 3.41 KK918 pKa = 9.76 FKK920 pKa = 11.16 QIMEE924 pKa = 4.32 NDD926 pKa = 3.25 LNMTKK931 pKa = 10.81 DD932 pKa = 4.12 KK933 pKa = 10.83 IFHH936 pKa = 5.84 NNKK939 pKa = 9.34 LKK941 pKa = 10.91 KK942 pKa = 10.57 LFGKK946 pKa = 10.42 KK947 pKa = 9.5 EE948 pKa = 3.43 IEE950 pKa = 4.22 YY951 pKa = 10.8 YY952 pKa = 9.91 IVTDD956 pKa = 3.51 IEE958 pKa = 4.15 HH959 pKa = 7.42 PIDD962 pKa = 3.55 VKK964 pKa = 11.01 YY965 pKa = 10.86 VQNQDD970 pKa = 3.55 KK971 pKa = 10.62 IINLPLYY978 pKa = 9.23 NQEE981 pKa = 3.64 IFEE984 pKa = 4.4 NEE986 pKa = 3.66 IQKK989 pKa = 10.13 IPDD992 pKa = 3.21 KK993 pKa = 10.79 DD994 pKa = 3.51 QNKK997 pKa = 8.99 IRR999 pKa = 11.84 NIHH1002 pKa = 5.86 LAAVEE1007 pKa = 4.31 IVVKK1011 pKa = 10.56 AYY1013 pKa = 9.98 FRR1015 pKa = 11.84 EE1016 pKa = 5.3 GIDD1019 pKa = 3.4 TPFEE1023 pKa = 4.97 IILCDD1028 pKa = 4.46 DD1029 pKa = 5.28 RR1030 pKa = 11.84 ITYY1033 pKa = 8.19 PQEE1036 pKa = 3.93 GSLVEE1041 pKa = 4.04 VLIGNLIYY1049 pKa = 10.74 QKK1051 pKa = 11.18 VKK1053 pKa = 7.8 FTKK1056 pKa = 10.08 IINYY1060 pKa = 9.62 SISIEE1065 pKa = 4.22 DD1066 pKa = 3.64 KK1067 pKa = 11.11 NLDD1070 pKa = 3.79 KK1071 pKa = 11.31 SLVMYY1076 pKa = 9.76 WNLEE1080 pKa = 4.14 GIKK1083 pKa = 9.47 MIKK1086 pKa = 9.7 DD1087 pKa = 3.32 SKK1089 pKa = 10.55 IFSIRR1094 pKa = 11.84 LRR1096 pKa = 11.84 NLYY1099 pKa = 10.04 VLSNKK1104 pKa = 10.14 HH1105 pKa = 4.53 IVKK1108 pKa = 10.39 NKK1110 pKa = 9.15 KK1111 pKa = 9.47 QYY1113 pKa = 10.13 NGNIIIEE1120 pKa = 4.78 PIFQDD1125 pKa = 4.23 VIQNNNRR1132 pKa = 11.84 NYY1134 pKa = 9.99 IEE1136 pKa = 3.96 YY1137 pKa = 10.02 GKK1139 pKa = 9.59 PGKK1142 pKa = 9.91 FDD1144 pKa = 3.29 RR1145 pKa = 11.84 TKK1147 pKa = 10.35 LKK1149 pKa = 10.55 SYY1151 pKa = 9.65 SRR1153 pKa = 11.84 RR1154 pKa = 11.84 FNEE1157 pKa = 4.02 PLRR1160 pKa = 11.84 LDD1162 pKa = 3.77 DD1163 pKa = 3.44 RR1164 pKa = 11.84 TNIQRR1169 pKa = 11.84 EE1170 pKa = 3.61 KK1171 pKa = 11.16 DD1172 pKa = 3.5 QIEE1175 pKa = 4.33 KK1176 pKa = 10.54 ADD1178 pKa = 4.19 HH1179 pKa = 6.08 NLEE1182 pKa = 4.11 LQKK1185 pKa = 10.77 EE1186 pKa = 4.46 LNNLNYY1192 pKa = 10.15 YY1193 pKa = 8.6 SQQGQSSNVLDD1204 pKa = 3.38 IPKK1207 pKa = 9.83 ILKK1210 pKa = 9.83 IEE1212 pKa = 4.1 NTKK1215 pKa = 10.74 NNYY1218 pKa = 8.84 KK1219 pKa = 9.84 QFHH1222 pKa = 7.04 IIGKK1226 pKa = 7.53 ITEE1229 pKa = 3.91 GRR1231 pKa = 11.84 LNKK1234 pKa = 10.08 FYY1236 pKa = 10.4 PILIDD1241 pKa = 3.14 TGAADD1246 pKa = 4.13 SYY1248 pKa = 10.81 ISSKK1252 pKa = 10.51 ILEE1255 pKa = 4.37 DD1256 pKa = 3.48 EE1257 pKa = 4.24 KK1258 pKa = 11.37 LVSNKK1263 pKa = 9.63 LSKK1266 pKa = 10.98 VVTSYY1271 pKa = 11.32 NADD1274 pKa = 3.79 NEE1276 pKa = 3.99 KK1277 pKa = 10.51 HH1278 pKa = 6.49 IYY1280 pKa = 9.71 DD1281 pKa = 4.31 RR1282 pKa = 11.84 NTEE1285 pKa = 4.17 VIIEE1289 pKa = 4.87 LIDD1292 pKa = 3.85 KK1293 pKa = 10.82 NNEE1296 pKa = 3.58 KK1297 pKa = 10.95 YY1298 pKa = 10.49 KK1299 pKa = 10.72 INFIGLVDD1307 pKa = 3.72 QLRR1310 pKa = 11.84 LLEE1313 pKa = 4.41 GGKK1316 pKa = 10.24 AEE1318 pKa = 4.65 ILLGMNILQNLKK1330 pKa = 9.74 PYY1332 pKa = 10.45 CITDD1336 pKa = 3.91 DD1337 pKa = 3.75 YY1338 pKa = 12.03 LEE1340 pKa = 4.52 INLGFRR1346 pKa = 11.84 CIKK1349 pKa = 9.76 INRR1352 pKa = 11.84 IKK1354 pKa = 10.81 KK1355 pKa = 9.89 DD1356 pKa = 2.85 IFEE1359 pKa = 4.2 IRR1361 pKa = 11.84 EE1362 pKa = 4.14 DD1363 pKa = 3.71 LQQMNVLNEE1372 pKa = 3.88
Molecular weight: 163.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.494
IPC2_protein 5.614
IPC_protein 5.614
Toseland 5.766
ProMoST 5.792
Dawson 5.703
Bjellqvist 5.779
Wikipedia 5.614
Rodwell 5.652
Grimsley 5.804
Solomon 5.703
Lehninger 5.664
Nozaki 5.881
DTASelect 6.02
Thurlkill 5.893
EMBOSS 5.83
Sillero 5.97
Patrickios 4.151
IPC_peptide 5.715
IPC2_peptide 5.944
IPC2.peptide.svr19 6.048
Protein with the highest isoelectric point:
>sp|Q89703|POL_CSVMV Putative enzymatic polyprotein OS=Cassava vein mosaic virus OX=38062 GN=ORF 3 PE=3 SV=1
MM1 pKa = 8.18 SLMNSTNPEE10 pKa = 4.01 FIEE13 pKa = 4.7 YY14 pKa = 10.51 YY15 pKa = 7.48 HH16 pKa = 6.43 TSWKK20 pKa = 7.94 PHH22 pKa = 6.38 KK23 pKa = 10.16 IEE25 pKa = 4.28 LVDD28 pKa = 4.75 NIYY31 pKa = 10.68 HH32 pKa = 5.96 SYY34 pKa = 10.64 GYY36 pKa = 10.11 YY37 pKa = 9.76 VYY39 pKa = 9.56 TRR41 pKa = 11.84 SVIKK45 pKa = 10.44 RR46 pKa = 11.84 FNKK49 pKa = 9.97 HH50 pKa = 6.22 LIKK53 pKa = 8.64 TTYY56 pKa = 10.36 KK57 pKa = 10.5 RR58 pKa = 11.84 IFSHH62 pKa = 6.87 PEE64 pKa = 3.41 NIVLHH69 pKa = 5.94 FRR71 pKa = 3.8
Molecular weight: 8.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.761
IPC2_protein 9.37
IPC_protein 9.37
Toseland 9.502
ProMoST 9.56
Dawson 9.853
Bjellqvist 9.604
Wikipedia 10.101
Rodwell 9.999
Grimsley 9.984
Solomon 9.897
Lehninger 9.853
Nozaki 9.458
DTASelect 9.604
Thurlkill 9.648
EMBOSS 9.94
Sillero 9.75
Patrickios 4.202
IPC_peptide 9.882
IPC2_peptide 8.287
IPC2.peptide.svr19 8.126
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2541
54
1372
508.2
60.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.44 ± 0.479
1.102 ± 0.429
5.037 ± 0.53
10.232 ± 1.528
3.109 ± 0.782
3.463 ± 0.36
1.889 ± 0.582
11.177 ± 0.88
11.885 ± 0.921
8.501 ± 1.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.046 ± 0.316
8.225 ± 1.016
3.266 ± 0.309
4.723 ± 1.207
3.463 ± 0.428
4.919 ± 0.542
3.975 ± 0.496
3.266 ± 0.697
1.023 ± 0.101
6.257 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here