Peptoanaerobacter stomatis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2275 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J5WP98|J5WP98_9FIRM HNH/ENDO VII family nuclease OS=Peptoanaerobacter stomatis OX=796937 GN=HMPREF1143_2241 PE=4 SV=1
MM1 pKa = 7.89 KK2 pKa = 10.43 YY3 pKa = 10.73 GSTPFEE9 pKa = 4.18 EE10 pKa = 5.29 IIFAMMLYY18 pKa = 10.09 VEE20 pKa = 4.58 HH21 pKa = 7.02 TDD23 pKa = 3.28 VSRR26 pKa = 11.84 LKK28 pKa = 9.66 EE29 pKa = 3.9 YY30 pKa = 11.2 KK31 pKa = 9.69 MNSLNSLISSRR42 pKa = 11.84 NEE44 pKa = 3.58 YY45 pKa = 10.3 YY46 pKa = 10.99 LEE48 pKa = 4.22 DD49 pKa = 3.96 TYY51 pKa = 11.83 DD52 pKa = 3.29 EE53 pKa = 4.64 YY54 pKa = 12.01 YY55 pKa = 11.06 EE56 pKa = 5.75 DD57 pKa = 4.22 VDD59 pKa = 5.6 DD60 pKa = 5.76 MLNEE64 pKa = 4.14 SNANFFDD71 pKa = 4.8 DD72 pKa = 3.71 SHH74 pKa = 7.58 FDD76 pKa = 3.75 SEE78 pKa = 5.57 SFTCYY83 pKa = 10.66 GILDD87 pKa = 4.81 DD88 pKa = 6.69 DD89 pKa = 5.44 DD90 pKa = 6.66 LSDD93 pKa = 3.98 YY94 pKa = 11.61 DD95 pKa = 4.7 KK96 pKa = 11.37 LLKK99 pKa = 10.56
Molecular weight: 11.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.935
Patrickios 0.528
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|G9XDJ6|G9XDJ6_9FIRM Enolase OS=Peptoanaerobacter stomatis OX=796937 GN=eno PE=3 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.96 RR4 pKa = 11.84 TYY6 pKa = 9.62 QPKK9 pKa = 9.59 KK10 pKa = 7.6 RR11 pKa = 11.84 QRR13 pKa = 11.84 SKK15 pKa = 9.69 EE16 pKa = 3.5 HH17 pKa = 6.13 GFRR20 pKa = 11.84 KK21 pKa = 9.98 RR22 pKa = 11.84 MRR24 pKa = 11.84 TPGGRR29 pKa = 11.84 NVIKK33 pKa = 10.45 NRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.79 GRR40 pKa = 11.84 KK41 pKa = 8.87 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.223
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.959
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2275
0
2275
720360
37
3711
316.6
35.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.598 ± 0.053
1.013 ± 0.021
6.24 ± 0.042
7.337 ± 0.063
4.39 ± 0.039
5.804 ± 0.052
1.334 ± 0.019
9.836 ± 0.067
9.573 ± 0.047
8.474 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.777 ± 0.025
6.23 ± 0.046
2.681 ± 0.025
2.875 ± 0.026
3.285 ± 0.036
6.519 ± 0.037
5.115 ± 0.044
6.045 ± 0.044
0.553 ± 0.015
4.322 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here