Fer-de-Lance paramyxovirus (strain ATCC VR-895) (FLDV) (Fer-de-Lance virus)
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6YIS1|Q6YIS1_FDLV8 Fusion glycoprotein F0 OS=Fer-de-Lance paramyxovirus (strain ATCC VR-895) OX=1283347 GN=F PE=3 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.49 PKK4 pKa = 10.77 SYY6 pKa = 11.08 YY7 pKa = 10.54 CNEE10 pKa = 4.36 DD11 pKa = 3.18 LRR13 pKa = 11.84 SDD15 pKa = 3.79 GGEE18 pKa = 3.92 KK19 pKa = 10.59 SPGGDD24 pKa = 3.17 LYY26 pKa = 11.32 KK27 pKa = 11.02 GIILVSTVISLIIAIISLAFIIDD50 pKa = 3.52 NKK52 pKa = 10.76 INIQSLDD59 pKa = 3.73 PLRR62 pKa = 11.84 GLEE65 pKa = 4.11 DD66 pKa = 3.63 SYY68 pKa = 11.85 LVPIKK73 pKa = 10.59 DD74 pKa = 3.36 KK75 pKa = 11.41 SEE77 pKa = 4.72 SISQDD82 pKa = 2.64 IQEE85 pKa = 5.07 GIFPRR90 pKa = 11.84 LNLITAATTTTIPRR104 pKa = 11.84 SIAIQTKK111 pKa = 10.09 DD112 pKa = 3.61 LSDD115 pKa = 5.41 LIMNRR120 pKa = 11.84 CYY122 pKa = 10.48 PSVVNNDD129 pKa = 3.33 TSCDD133 pKa = 3.57 VLAGAIHH140 pKa = 6.76 SNLFSQLDD148 pKa = 3.65 PSTYY152 pKa = 6.92 WTCSSGTPTMNQTVKK167 pKa = 10.92 LLPDD171 pKa = 3.63 NSQIPGSTYY180 pKa = 9.1 STGCVRR186 pKa = 11.84 IPTFSLGSMIYY197 pKa = 9.69 SYY199 pKa = 10.73 SHH201 pKa = 5.07 NVIYY205 pKa = 9.98 EE206 pKa = 3.91 GCNDD210 pKa = 3.42 HH211 pKa = 7.04 SKK213 pKa = 10.93 SSQYY217 pKa = 8.87 WQLGYY222 pKa = 10.41 ISTSKK227 pKa = 9.57 TGEE230 pKa = 3.85 PLQQVSRR237 pKa = 11.84 TLTLNNGLNRR247 pKa = 11.84 KK248 pKa = 8.96 SCSTVAQGRR257 pKa = 11.84 GAYY260 pKa = 9.35 LLCTNVVEE268 pKa = 5.87 DD269 pKa = 4.05 EE270 pKa = 4.2 RR271 pKa = 11.84 TDD273 pKa = 3.48 YY274 pKa = 10.32 STEE277 pKa = 4.35 GIQDD281 pKa = 3.51 LTLDD285 pKa = 4.05 YY286 pKa = 10.95 IDD288 pKa = 3.94 IFGAEE293 pKa = 3.34 RR294 pKa = 11.84 SYY296 pKa = 11.1 RR297 pKa = 11.84 YY298 pKa = 8.44 TNNEE302 pKa = 3.16 VDD304 pKa = 3.94 LDD306 pKa = 3.81 RR307 pKa = 11.84 PYY309 pKa = 11.27 AALYY313 pKa = 9.38 PSVGSGTVYY322 pKa = 10.77 NDD324 pKa = 3.84 RR325 pKa = 11.84 ILFLGYY331 pKa = 10.09 GGLMTPYY338 pKa = 10.36 GDD340 pKa = 4.0 QAMCQAPEE348 pKa = 4.39 CTSATQEE355 pKa = 4.28 GCNSNQLIGYY365 pKa = 8.23 FSGRR369 pKa = 11.84 QIVNCIIEE377 pKa = 4.81 IITVGTEE384 pKa = 3.54 KK385 pKa = 10.65 PIIRR389 pKa = 11.84 VRR391 pKa = 11.84 TIPNSQVWLGAEE403 pKa = 4.28 GRR405 pKa = 11.84 IQTLGGVLYY414 pKa = 10.43 LYY416 pKa = 10.08 IRR418 pKa = 11.84 SSGWHH423 pKa = 6.36 ALAQTGIILTLDD435 pKa = 4.84 PIRR438 pKa = 11.84 ISWIVNTGYY447 pKa = 10.51 SRR449 pKa = 11.84 PGNGPCSASSRR460 pKa = 11.84 CPAQCITGVYY470 pKa = 9.63 TDD472 pKa = 4.94 IFPLSQNYY480 pKa = 9.96 GYY482 pKa = 10.66 LATVTLLSGVDD493 pKa = 3.17 RR494 pKa = 11.84 VNPVISYY501 pKa = 7.4 GTSTGRR507 pKa = 11.84 VADD510 pKa = 3.87 SQLTSSSQVAAYY522 pKa = 7.11 TTTTCFTFNQKK533 pKa = 10.0 GYY535 pKa = 9.02 CYY537 pKa = 10.0 HH538 pKa = 7.93 IIEE541 pKa = 5.32 LSPATLGIFQPVLVVTEE558 pKa = 4.09 IPKK561 pKa = 9.79 ICSS564 pKa = 3.11
Molecular weight: 61.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.053
IPC2_protein 5.054
IPC_protein 4.991
Toseland 4.876
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.876
Rodwell 4.876
Grimsley 4.8
Solomon 4.991
Lehninger 4.94
Nozaki 5.105
DTASelect 5.308
Thurlkill 4.902
EMBOSS 4.914
Sillero 5.156
Patrickios 0.566
IPC_peptide 4.991
IPC2_peptide 5.156
IPC2.peptide.svr19 5.008
Protein with the highest isoelectric point:
>tr|Q6YIS6|Q6YIS6_FDLV8 Nucleocapsid OS=Fer-de-Lance paramyxovirus (strain ATCC VR-895) OX=1283347 GN=NP PE=3 SV=1
MM1 pKa = 7.27 IRR3 pKa = 11.84 TRR5 pKa = 11.84 IYY7 pKa = 10.39 KK8 pKa = 8.35 PTYY11 pKa = 6.94 TTTTPPTCHH20 pKa = 6.15 TPIKK24 pKa = 9.83 MEE26 pKa = 4.15 EE27 pKa = 4.0 DD28 pKa = 3.7 PRR30 pKa = 11.84 EE31 pKa = 4.05 KK32 pKa = 9.72 MHH34 pKa = 6.32 PQSMWRR40 pKa = 11.84 LVRR43 pKa = 11.84 LRR45 pKa = 11.84 AQRR48 pKa = 11.84 LLSYY52 pKa = 10.72 SEE54 pKa = 4.16 STDD57 pKa = 3.08 LSTRR61 pKa = 11.84 EE62 pKa = 3.85 FLEE65 pKa = 4.51 DD66 pKa = 3.24 VSKK69 pKa = 11.25 SVVVLFNRR77 pKa = 11.84 DD78 pKa = 3.08 GMSSISQWRR87 pKa = 11.84 TEE89 pKa = 3.85 DD90 pKa = 3.29 CAARR94 pKa = 11.84 RR95 pKa = 11.84 LGNLSKK101 pKa = 10.44 FAWDD105 pKa = 3.34 AVTKK109 pKa = 10.83 GRR111 pKa = 11.84 MDD113 pKa = 3.86 PCRR116 pKa = 11.84 LAFKK120 pKa = 10.18 MVTEE124 pKa = 5.03 LGNDD128 pKa = 3.15 VAIRR132 pKa = 11.84 AEE134 pKa = 3.81 ILTVVWLITGWSTIPRR150 pKa = 11.84 TLHH153 pKa = 5.97 KK154 pKa = 10.29 DD155 pKa = 2.99 LWSSAIYY162 pKa = 10.06 RR163 pKa = 11.84 RR164 pKa = 11.84 LSLL167 pKa = 4.06
Molecular weight: 19.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.048
IPC_protein 9.151
Toseland 9.809
ProMoST 9.531
Dawson 10.028
Bjellqvist 9.75
Wikipedia 10.204
Rodwell 10.292
Grimsley 10.101
Solomon 10.087
Lehninger 10.058
Nozaki 9.911
DTASelect 9.721
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.97
Patrickios 9.78
IPC_peptide 10.087
IPC2_peptide 8.609
IPC2.peptide.svr19 7.905
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4935
167
2181
616.9
68.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.694 ± 0.775
1.743 ± 0.274
5.39 ± 0.18
5.897 ± 0.639
3.019 ± 0.344
6.403 ± 0.542
1.662 ± 0.33
8.186 ± 0.52
5.694 ± 0.483
9.463 ± 0.784
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.614 ± 0.314
4.721 ± 0.313
4.093 ± 0.547
3.708 ± 0.258
4.965 ± 0.488
8.936 ± 0.247
7.295 ± 0.613
6.444 ± 0.354
0.993 ± 0.147
3.08 ± 0.655
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here