Clostridium sp. CAG:465

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6UA01|R6UA01_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:465 OX=1262811 GN=BN667_00464 PE=4 SV=1
MM1 pKa = 7.37SVQHH5 pKa = 6.82NIQITNGKK13 pKa = 9.96GSLALANGNYY23 pKa = 9.25TITAEE28 pKa = 4.0AFGYY32 pKa = 10.03NPPSLDD38 pKa = 3.36PSTIEE43 pKa = 4.03IIEE46 pKa = 4.24GKK48 pKa = 9.97NEE50 pKa = 3.66YY51 pKa = 10.56SFTISATGTLTLHH64 pKa = 5.89ITDD67 pKa = 4.15NGTAGGVPIEE77 pKa = 4.17GATFYY82 pKa = 11.1RR83 pKa = 11.84CDD85 pKa = 3.94AEE87 pKa = 4.28GNTYY91 pKa = 11.15GDD93 pKa = 4.47IITSNVDD100 pKa = 2.36GDD102 pKa = 4.91AIFNNVPYY110 pKa = 10.42SADD113 pKa = 3.42VTPLSVYY120 pKa = 10.03FKK122 pKa = 10.49QVSSDD127 pKa = 3.68GEE129 pKa = 4.22HH130 pKa = 6.05TFNAEE135 pKa = 4.06LQNTTLQDD143 pKa = 3.44QEE145 pKa = 4.21VTLQISNPDD154 pKa = 2.95ATEE157 pKa = 3.82RR158 pKa = 11.84TFTFTDD164 pKa = 3.03KK165 pKa = 11.19NYY167 pKa = 11.04ANLPIANGKK176 pKa = 9.63LIAEE180 pKa = 4.74GG181 pKa = 3.45

Molecular weight: 19.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6TYC9|R6TYC9_9CLOT Putative divalent heavy-metal cations transporter OS=Clostridium sp. CAG:465 OX=1262811 GN=BN667_00361 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 9.29KK9 pKa = 7.55IHH11 pKa = 6.2RR12 pKa = 11.84EE13 pKa = 3.75KK14 pKa = 10.51VHH16 pKa = 5.97GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTASGRR28 pKa = 11.84KK29 pKa = 7.2VLKK32 pKa = 10.3SRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.07GRR39 pKa = 11.84KK40 pKa = 8.58VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1173

0

1173

334682

31

1867

285.3

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.732 ± 0.072

1.266 ± 0.029

5.981 ± 0.055

7.284 ± 0.077

4.191 ± 0.055

5.367 ± 0.073

1.14 ± 0.023

10.304 ± 0.089

10.401 ± 0.078

8.326 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.026

7.505 ± 0.09

2.364 ± 0.035

2.19 ± 0.038

3.091 ± 0.043

6.473 ± 0.058

5.133 ± 0.057

6.571 ± 0.064

0.497 ± 0.017

4.67 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski