Amino acid dipepetide frequency for Bacillus phage vB_BtS_B83

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.507AlaAla: 6.507 ± 0.889
0.407AlaCys: 0.407 ± 0.175
3.999AlaAsp: 3.999 ± 0.454
5.49AlaGlu: 5.49 ± 0.643
2.508AlaPhe: 2.508 ± 0.567
4.473AlaGly: 4.473 ± 0.998
1.22AlaHis: 1.22 ± 0.249
6.032AlaIle: 6.032 ± 0.701
4.609AlaLys: 4.609 ± 0.555
5.626AlaLeu: 5.626 ± 0.732
1.694AlaMet: 1.694 ± 0.29
3.524AlaAsn: 3.524 ± 0.534
2.033AlaPro: 2.033 ± 0.465
2.304AlaGln: 2.304 ± 0.81
3.389AlaArg: 3.389 ± 0.469
3.321AlaSer: 3.321 ± 0.557
4.338AlaThr: 4.338 ± 0.529
4.677AlaVal: 4.677 ± 0.584
0.474AlaTrp: 0.474 ± 0.223
1.762AlaTyr: 1.762 ± 0.245
0.0AlaXaa: 0.0 ± 0.0
Cys
0.271CysAla: 0.271 ± 0.131
0.203CysCys: 0.203 ± 0.107
0.542CysAsp: 0.542 ± 0.23
0.746CysGlu: 0.746 ± 0.251
0.339CysPhe: 0.339 ± 0.153
0.813CysGly: 0.813 ± 0.291
0.271CysHis: 0.271 ± 0.145
0.746CysIle: 0.746 ± 0.282
1.017CysLys: 1.017 ± 0.366
0.542CysLeu: 0.542 ± 0.235
0.136CysMet: 0.136 ± 0.093
0.542CysAsn: 0.542 ± 0.221
0.339CysPro: 0.339 ± 0.158
0.474CysGln: 0.474 ± 0.187
0.271CysArg: 0.271 ± 0.12
0.271CysSer: 0.271 ± 0.114
0.474CysThr: 0.474 ± 0.161
0.474CysVal: 0.474 ± 0.192
0.068CysTrp: 0.068 ± 0.079
0.746CysTyr: 0.746 ± 0.273
0.0CysXaa: 0.0 ± 0.0
Asp
3.931AspAla: 3.931 ± 0.397
0.339AspCys: 0.339 ± 0.158
3.118AspAsp: 3.118 ± 0.597
4.338AspGlu: 4.338 ± 0.602
2.847AspPhe: 2.847 ± 0.512
3.796AspGly: 3.796 ± 0.521
0.407AspHis: 0.407 ± 0.192
5.761AspIle: 5.761 ± 0.637
5.422AspLys: 5.422 ± 0.496
4.677AspLeu: 4.677 ± 0.551
1.559AspMet: 1.559 ± 0.282
2.982AspAsn: 2.982 ± 0.349
2.033AspPro: 2.033 ± 0.342
1.966AspGln: 1.966 ± 0.356
2.847AspArg: 2.847 ± 0.416
2.576AspSer: 2.576 ± 0.416
3.592AspThr: 3.592 ± 0.692
4.338AspVal: 4.338 ± 0.566
0.813AspTrp: 0.813 ± 0.174
2.779AspTyr: 2.779 ± 0.457
0.0AspXaa: 0.0 ± 0.0
Glu
5.083GluAla: 5.083 ± 0.558
0.746GluCys: 0.746 ± 0.249
4.677GluAsp: 4.677 ± 0.685
8.133GluGlu: 8.133 ± 0.951
2.508GluPhe: 2.508 ± 0.507
4.812GluGly: 4.812 ± 0.57
1.084GluHis: 1.084 ± 0.28
7.862GluIle: 7.862 ± 0.564
8.608GluLys: 8.608 ± 0.793
7.456GluLeu: 7.456 ± 0.763
2.779GluMet: 2.779 ± 0.505
4.406GluAsn: 4.406 ± 0.38
1.898GluPro: 1.898 ± 0.449
4.134GluGln: 4.134 ± 0.587
3.05GluArg: 3.05 ± 0.448
4.541GluSer: 4.541 ± 0.573
4.067GluThr: 4.067 ± 0.436
5.558GluVal: 5.558 ± 0.542
1.152GluTrp: 1.152 ± 0.224
2.711GluTyr: 2.711 ± 0.55
0.0GluXaa: 0.0 ± 0.0
Phe
2.237PheAla: 2.237 ± 0.437
0.203PheCys: 0.203 ± 0.117
2.372PheAsp: 2.372 ± 0.416
2.643PheGlu: 2.643 ± 0.369
1.356PhePhe: 1.356 ± 0.382
2.237PheGly: 2.237 ± 0.322
0.813PheHis: 0.813 ± 0.217
2.508PheIle: 2.508 ± 0.44
3.524PheLys: 3.524 ± 0.43
3.253PheLeu: 3.253 ± 0.512
1.288PheMet: 1.288 ± 0.331
1.83PheAsn: 1.83 ± 0.346
0.813PhePro: 0.813 ± 0.224
1.22PheGln: 1.22 ± 0.248
1.491PheArg: 1.491 ± 0.242
2.508PheSer: 2.508 ± 0.426
1.898PheThr: 1.898 ± 0.252
2.576PheVal: 2.576 ± 0.422
0.474PheTrp: 0.474 ± 0.223
1.356PheTyr: 1.356 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
3.728GlyAla: 3.728 ± 0.69
0.61GlyCys: 0.61 ± 0.223
3.389GlyAsp: 3.389 ± 0.488
4.88GlyGlu: 4.88 ± 0.576
2.508GlyPhe: 2.508 ± 0.507
3.796GlyGly: 3.796 ± 0.532
1.084GlyHis: 1.084 ± 0.262
5.49GlyIle: 5.49 ± 0.687
5.761GlyLys: 5.761 ± 0.651
4.473GlyLeu: 4.473 ± 0.875
1.694GlyMet: 1.694 ± 0.394
4.067GlyAsn: 4.067 ± 0.623
0.881GlyPro: 0.881 ± 0.194
2.101GlyGln: 2.101 ± 0.333
2.914GlyArg: 2.914 ± 0.471
3.186GlySer: 3.186 ± 0.531
3.66GlyThr: 3.66 ± 0.637
3.389GlyVal: 3.389 ± 0.496
1.288GlyTrp: 1.288 ± 0.289
1.966GlyTyr: 1.966 ± 0.316
0.0GlyXaa: 0.0 ± 0.0
His
0.542HisAla: 0.542 ± 0.164
0.339HisCys: 0.339 ± 0.156
0.813HisAsp: 0.813 ± 0.222
1.084HisGlu: 1.084 ± 0.29
1.017HisPhe: 1.017 ± 0.184
0.339HisGly: 0.339 ± 0.162
0.474HisHis: 0.474 ± 0.183
1.423HisIle: 1.423 ± 0.277
0.949HisLys: 0.949 ± 0.278
0.813HisLeu: 0.813 ± 0.239
0.474HisMet: 0.474 ± 0.168
1.152HisAsn: 1.152 ± 0.266
0.474HisPro: 0.474 ± 0.183
0.542HisGln: 0.542 ± 0.281
0.542HisArg: 0.542 ± 0.158
0.746HisSer: 0.746 ± 0.179
0.813HisThr: 0.813 ± 0.199
1.288HisVal: 1.288 ± 0.293
0.339HisTrp: 0.339 ± 0.125
0.474HisTyr: 0.474 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
6.032IleAla: 6.032 ± 0.657
1.152IleCys: 1.152 ± 0.285
5.287IleAsp: 5.287 ± 0.634
7.862IleGlu: 7.862 ± 0.753
1.356IlePhe: 1.356 ± 0.309
3.321IleGly: 3.321 ± 0.689
1.288IleHis: 1.288 ± 0.242
4.744IleIle: 4.744 ± 0.677
6.574IleLys: 6.574 ± 0.55
4.744IleLeu: 4.744 ± 0.678
1.694IleMet: 1.694 ± 0.299
5.083IleAsn: 5.083 ± 0.546
2.982IlePro: 2.982 ± 0.419
3.66IleGln: 3.66 ± 0.577
2.847IleArg: 2.847 ± 0.496
4.541IleSer: 4.541 ± 0.775
4.406IleThr: 4.406 ± 0.621
3.728IleVal: 3.728 ± 0.453
1.017IleTrp: 1.017 ± 0.267
1.762IleTyr: 1.762 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
6.71LysAla: 6.71 ± 1.005
0.813LysCys: 0.813 ± 0.299
5.016LysAsp: 5.016 ± 0.631
8.676LysGlu: 8.676 ± 0.773
2.304LysPhe: 2.304 ± 0.355
5.422LysGly: 5.422 ± 0.52
1.22LysHis: 1.22 ± 0.278
5.49LysIle: 5.49 ± 0.558
7.998LysLys: 7.998 ± 0.676
7.591LysLeu: 7.591 ± 0.742
3.118LysMet: 3.118 ± 0.419
3.999LysAsn: 3.999 ± 0.45
2.101LysPro: 2.101 ± 0.441
3.931LysGln: 3.931 ± 0.579
4.541LysArg: 4.541 ± 0.617
5.354LysSer: 5.354 ± 0.572
4.744LysThr: 4.744 ± 0.601
6.439LysVal: 6.439 ± 0.857
1.627LysTrp: 1.627 ± 0.332
3.321LysTyr: 3.321 ± 0.432
0.0LysXaa: 0.0 ± 0.0
Leu
4.88LeuAla: 4.88 ± 0.575
0.949LeuCys: 0.949 ± 0.351
5.422LeuAsp: 5.422 ± 0.639
7.456LeuGlu: 7.456 ± 0.703
2.643LeuPhe: 2.643 ± 0.4
4.27LeuGly: 4.27 ± 0.691
1.356LeuHis: 1.356 ± 0.379
4.677LeuIle: 4.677 ± 0.553
7.252LeuLys: 7.252 ± 0.635
5.897LeuLeu: 5.897 ± 0.652
1.898LeuMet: 1.898 ± 0.355
4.338LeuAsn: 4.338 ± 0.529
2.372LeuPro: 2.372 ± 0.449
3.728LeuGln: 3.728 ± 0.515
4.27LeuArg: 4.27 ± 0.574
4.88LeuSer: 4.88 ± 0.683
4.677LeuThr: 4.677 ± 0.53
4.202LeuVal: 4.202 ± 0.643
0.746LeuTrp: 0.746 ± 0.226
2.711LeuTyr: 2.711 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
1.356MetAla: 1.356 ± 0.305
0.203MetCys: 0.203 ± 0.106
1.559MetAsp: 1.559 ± 0.299
2.304MetGlu: 2.304 ± 0.334
1.084MetPhe: 1.084 ± 0.271
1.423MetGly: 1.423 ± 0.356
0.474MetHis: 0.474 ± 0.168
1.559MetIle: 1.559 ± 0.299
2.847MetLys: 2.847 ± 0.363
1.762MetLeu: 1.762 ± 0.306
0.407MetMet: 0.407 ± 0.157
1.491MetAsn: 1.491 ± 0.474
0.881MetPro: 0.881 ± 0.2
1.627MetGln: 1.627 ± 0.324
0.881MetArg: 0.881 ± 0.216
2.169MetSer: 2.169 ± 0.447
1.288MetThr: 1.288 ± 0.396
2.237MetVal: 2.237 ± 0.43
0.407MetTrp: 0.407 ± 0.192
1.017MetTyr: 1.017 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
4.541AsnAla: 4.541 ± 0.63
0.542AsnCys: 0.542 ± 0.223
1.966AsnAsp: 1.966 ± 0.298
4.406AsnGlu: 4.406 ± 0.569
1.762AsnPhe: 1.762 ± 0.322
4.541AsnGly: 4.541 ± 0.839
0.746AsnHis: 0.746 ± 0.231
3.863AsnIle: 3.863 ± 0.413
5.693AsnLys: 5.693 ± 0.656
5.016AsnLeu: 5.016 ± 0.626
1.22AsnMet: 1.22 ± 0.282
2.643AsnAsn: 2.643 ± 0.429
1.288AsnPro: 1.288 ± 0.31
1.491AsnGln: 1.491 ± 0.298
2.304AsnArg: 2.304 ± 0.426
3.118AsnSer: 3.118 ± 0.457
3.321AsnThr: 3.321 ± 0.495
3.389AsnVal: 3.389 ± 0.447
0.61AsnTrp: 0.61 ± 0.198
2.033AsnTyr: 2.033 ± 0.417
0.0AsnXaa: 0.0 ± 0.0
Pro
2.101ProAla: 2.101 ± 0.267
0.339ProCys: 0.339 ± 0.148
1.694ProAsp: 1.694 ± 0.331
2.169ProGlu: 2.169 ± 0.355
1.084ProPhe: 1.084 ± 0.331
1.491ProGly: 1.491 ± 0.4
0.203ProHis: 0.203 ± 0.112
1.966ProIle: 1.966 ± 0.503
2.033ProLys: 2.033 ± 0.284
2.101ProLeu: 2.101 ± 0.428
0.813ProMet: 0.813 ± 0.225
1.356ProAsn: 1.356 ± 0.252
0.949ProPro: 0.949 ± 0.274
0.813ProGln: 0.813 ± 0.232
1.22ProArg: 1.22 ± 0.335
1.627ProSer: 1.627 ± 0.272
2.237ProThr: 2.237 ± 0.422
1.898ProVal: 1.898 ± 0.371
0.271ProTrp: 0.271 ± 0.122
1.762ProTyr: 1.762 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
1.491GlnAla: 1.491 ± 0.28
0.407GlnCys: 0.407 ± 0.14
2.169GlnAsp: 2.169 ± 0.386
3.728GlnGlu: 3.728 ± 0.486
0.949GlnPhe: 0.949 ± 0.235
2.711GlnGly: 2.711 ± 0.495
0.474GlnHis: 0.474 ± 0.153
2.982GlnIle: 2.982 ± 0.352
3.321GlnLys: 3.321 ± 0.475
3.389GlnLeu: 3.389 ± 0.493
0.881GlnMet: 0.881 ± 0.215
2.508GlnAsn: 2.508 ± 0.399
1.288GlnPro: 1.288 ± 0.301
2.508GlnGln: 2.508 ± 0.395
2.101GlnArg: 2.101 ± 0.353
2.44GlnSer: 2.44 ± 0.507
2.033GlnThr: 2.033 ± 0.641
2.643GlnVal: 2.643 ± 0.429
0.542GlnTrp: 0.542 ± 0.155
1.288GlnTyr: 1.288 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
1.898ArgAla: 1.898 ± 0.32
0.339ArgCys: 0.339 ± 0.188
3.186ArgAsp: 3.186 ± 0.496
3.457ArgGlu: 3.457 ± 0.499
2.779ArgPhe: 2.779 ± 0.453
2.914ArgGly: 2.914 ± 0.411
0.746ArgHis: 0.746 ± 0.25
3.321ArgIle: 3.321 ± 0.521
4.541ArgLys: 4.541 ± 0.806
3.186ArgLeu: 3.186 ± 0.451
1.084ArgMet: 1.084 ± 0.257
2.779ArgAsn: 2.779 ± 0.377
0.949ArgPro: 0.949 ± 0.267
1.694ArgGln: 1.694 ± 0.363
2.169ArgArg: 2.169 ± 0.315
2.643ArgSer: 2.643 ± 0.531
1.694ArgThr: 1.694 ± 0.255
3.321ArgVal: 3.321 ± 0.484
0.61ArgTrp: 0.61 ± 0.176
2.101ArgTyr: 2.101 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
3.05SerAla: 3.05 ± 0.685
0.271SerCys: 0.271 ± 0.156
3.389SerAsp: 3.389 ± 0.453
4.338SerGlu: 4.338 ± 0.574
2.44SerPhe: 2.44 ± 0.411
4.473SerGly: 4.473 ± 0.573
0.949SerHis: 0.949 ± 0.275
4.202SerIle: 4.202 ± 0.568
4.88SerLys: 4.88 ± 0.558
3.999SerLeu: 3.999 ± 0.533
1.898SerMet: 1.898 ± 0.385
3.592SerAsn: 3.592 ± 0.563
1.627SerPro: 1.627 ± 0.321
2.101SerGln: 2.101 ± 0.415
2.508SerArg: 2.508 ± 0.447
2.914SerSer: 2.914 ± 0.386
3.66SerThr: 3.66 ± 0.493
4.067SerVal: 4.067 ± 0.554
0.678SerTrp: 0.678 ± 0.178
1.898SerTyr: 1.898 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
5.626ThrAla: 5.626 ± 0.979
0.271ThrCys: 0.271 ± 0.14
3.796ThrAsp: 3.796 ± 0.501
4.27ThrGlu: 4.27 ± 0.533
2.033ThrPhe: 2.033 ± 0.38
4.067ThrGly: 4.067 ± 0.448
0.474ThrHis: 0.474 ± 0.18
4.202ThrIle: 4.202 ± 0.591
5.626ThrLys: 5.626 ± 1.008
4.812ThrLeu: 4.812 ± 0.44
1.423ThrMet: 1.423 ± 0.29
3.05ThrAsn: 3.05 ± 0.435
1.762ThrPro: 1.762 ± 0.455
1.559ThrGln: 1.559 ± 0.528
1.898ThrArg: 1.898 ± 0.294
2.982ThrSer: 2.982 ± 0.507
3.999ThrThr: 3.999 ± 0.573
3.186ThrVal: 3.186 ± 0.451
0.61ThrTrp: 0.61 ± 0.18
1.762ThrTyr: 1.762 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
5.558ValAla: 5.558 ± 0.613
0.542ValCys: 0.542 ± 0.184
4.812ValAsp: 4.812 ± 0.528
5.422ValGlu: 5.422 ± 0.525
2.576ValPhe: 2.576 ± 0.344
2.711ValGly: 2.711 ± 0.412
0.746ValHis: 0.746 ± 0.294
3.999ValIle: 3.999 ± 0.548
6.1ValLys: 6.1 ± 0.71
4.812ValLeu: 4.812 ± 0.574
1.423ValMet: 1.423 ± 0.325
3.321ValAsn: 3.321 ± 0.586
2.508ValPro: 2.508 ± 0.537
2.711ValGln: 2.711 ± 0.483
3.457ValArg: 3.457 ± 0.478
4.406ValSer: 4.406 ± 0.536
3.66ValThr: 3.66 ± 0.687
3.728ValVal: 3.728 ± 0.538
0.678ValTrp: 0.678 ± 0.238
1.423ValTyr: 1.423 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
0.61TrpAla: 0.61 ± 0.202
0.271TrpCys: 0.271 ± 0.191
1.22TrpAsp: 1.22 ± 0.37
1.288TrpGlu: 1.288 ± 0.322
0.61TrpPhe: 0.61 ± 0.21
0.474TrpGly: 0.474 ± 0.191
0.068TrpHis: 0.068 ± 0.063
1.084TrpIle: 1.084 ± 0.311
1.084TrpLys: 1.084 ± 0.302
1.288TrpLeu: 1.288 ± 0.23
0.068TrpMet: 0.068 ± 0.05
0.61TrpAsn: 0.61 ± 0.211
0.0TrpPro: 0.0 ± 0.0
0.407TrpGln: 0.407 ± 0.17
0.813TrpArg: 0.813 ± 0.224
0.678TrpSer: 0.678 ± 0.222
0.61TrpThr: 0.61 ± 0.21
0.949TrpVal: 0.949 ± 0.259
0.271TrpTrp: 0.271 ± 0.144
0.542TrpTyr: 0.542 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.237TyrAla: 2.237 ± 0.364
0.339TyrCys: 0.339 ± 0.161
1.694TyrAsp: 1.694 ± 0.336
2.576TyrGlu: 2.576 ± 0.427
1.898TyrPhe: 1.898 ± 0.437
2.779TyrGly: 2.779 ± 0.329
0.542TyrHis: 0.542 ± 0.185
2.169TyrIle: 2.169 ± 0.391
2.643TyrLys: 2.643 ± 0.398
3.186TyrLeu: 3.186 ± 0.442
1.356TyrMet: 1.356 ± 0.333
1.22TyrAsn: 1.22 ± 0.299
0.949TyrPro: 0.949 ± 0.26
0.881TyrGln: 0.881 ± 0.202
1.898TyrArg: 1.898 ± 0.4
2.033TyrSer: 2.033 ± 0.338
2.237TyrThr: 2.237 ± 0.38
2.508TyrVal: 2.508 ± 0.39
0.271TyrTrp: 0.271 ± 0.123
1.559TyrTyr: 1.559 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (14755 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski