Rhodococcus sp. C1-24

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A7S3W4|A0A5A7S3W4_9NOCA Glycoside hydrolase family 15 protein OS=Rhodococcus sp. C1-24 OX=2495913 GN=FOY51_26120 PE=4 SV=1
MM1 pKa = 7.75FDD3 pKa = 3.16QHH5 pKa = 8.22LLIAALSRR13 pKa = 11.84YY14 pKa = 9.07VRR16 pKa = 11.84TLPTLYY22 pKa = 10.36EE23 pKa = 4.23SEE25 pKa = 4.52FVLDD29 pKa = 4.96DD30 pKa = 4.07LADD33 pKa = 3.66TVTEE37 pKa = 4.09VLGVTGAGVSLVSDD51 pKa = 3.38EE52 pKa = 4.64RR53 pKa = 11.84LWFVTSSTGNMVEE66 pKa = 3.94VEE68 pKa = 4.53LWQEE72 pKa = 4.51HH73 pKa = 5.41YY74 pKa = 10.82QDD76 pKa = 3.79GPARR80 pKa = 11.84TAYY83 pKa = 8.04EE84 pKa = 3.78TDD86 pKa = 3.36APVCITDD93 pKa = 4.69IGDD96 pKa = 4.33CGDD99 pKa = 3.46RR100 pKa = 11.84WPLYY104 pKa = 10.36AAAAHH109 pKa = 6.61DD110 pKa = 4.41AGVVAVGAIPMRR122 pKa = 11.84LGDD125 pKa = 4.93DD126 pKa = 3.71IIGVLDD132 pKa = 5.15LYY134 pKa = 11.32ADD136 pKa = 4.97DD137 pKa = 4.27PRR139 pKa = 11.84AWPAPDD145 pKa = 3.0IAAAQLLADD154 pKa = 3.92IATGYY159 pKa = 9.95LVNASTPAQHH169 pKa = 6.33TEE171 pKa = 3.12IDD173 pKa = 3.52IYY175 pKa = 11.06NAARR179 pKa = 11.84TVPAVDD185 pKa = 3.17

Molecular weight: 19.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A7SFX3|A0A5A7SFX3_9NOCA Thiolase family protein OS=Rhodococcus sp. C1-24 OX=2495913 GN=FOY51_04865 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.93GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5215

0

5215

1709589

14

8731

327.8

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.7 ± 0.045

0.746 ± 0.012

6.579 ± 0.029

5.119 ± 0.028

3.096 ± 0.019

8.764 ± 0.033

2.14 ± 0.016

4.564 ± 0.021

2.431 ± 0.028

9.894 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.944 ± 0.014

2.269 ± 0.017

5.424 ± 0.03

2.955 ± 0.018

7.043 ± 0.032

5.856 ± 0.022

6.189 ± 0.024

8.796 ± 0.031

1.434 ± 0.015

2.055 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski