Actinoplanes phage phiAsp2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6J816|Q6J816_9CAUD Pas15 OS=Actinoplanes phage phiAsp2 OX=279303 GN=pas15 PE=4 SV=1
MM1 pKa = 7.45NLLCAKK7 pKa = 9.26PLQGEE12 pKa = 4.71TIRR15 pKa = 11.84VTRR18 pKa = 11.84LDD20 pKa = 3.32EE21 pKa = 4.68CGNPEE26 pKa = 4.85FGDD29 pKa = 4.01CAFGVSDD36 pKa = 4.8GYY38 pKa = 11.81VSVTLTPNTEE48 pKa = 3.63EE49 pKa = 4.78GEE51 pKa = 4.35RR52 pKa = 11.84FLQRR56 pKa = 11.84NANGRR61 pKa = 11.84AIVNQRR67 pKa = 11.84SAPSLNWYY75 pKa = 9.3DD76 pKa = 3.38VSIVFQEE83 pKa = 3.66VDD85 pKa = 3.26AEE87 pKa = 4.32LFTIVTGLQPYY98 pKa = 10.72LDD100 pKa = 4.66DD101 pKa = 3.64QDD103 pKa = 4.37NVIGFPVTEE112 pKa = 4.52SDD114 pKa = 3.51FATANFALEE123 pKa = 3.78AWMGNAEE130 pKa = 4.33EE131 pKa = 4.47EE132 pKa = 4.77CLPGDD137 pKa = 3.29QFPFYY142 pKa = 10.6GYY144 pKa = 11.14NLLPWVVEE152 pKa = 4.32GALSEE157 pKa = 5.37DD158 pKa = 3.23IVITNDD164 pKa = 4.04LITFTVMGRR173 pKa = 11.84TRR175 pKa = 11.84KK176 pKa = 7.35GTPWGVGPYY185 pKa = 10.51DD186 pKa = 3.5VVRR189 pKa = 11.84DD190 pKa = 3.96SGGDD194 pKa = 3.5PSPLFTAIPSDD205 pKa = 3.8THH207 pKa = 6.72HH208 pKa = 6.75LPIWTQLAPPAAEE221 pKa = 4.9CGCQALSSS229 pKa = 3.85

Molecular weight: 25.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6J830|Q6J830_9CAUD Pas1 OS=Actinoplanes phage phiAsp2 OX=279303 GN=pas1 PE=4 SV=1
MM1 pKa = 7.61ANGVVTGDD9 pKa = 3.35PGQVYY14 pKa = 10.49GRR16 pKa = 11.84IRR18 pKa = 11.84VADD21 pKa = 3.64VVLAIRR27 pKa = 11.84DD28 pKa = 4.06LEE30 pKa = 4.63PGFHH34 pKa = 6.22TVTEE38 pKa = 3.97VHH40 pKa = 6.47RR41 pKa = 11.84LYY43 pKa = 10.92LARR46 pKa = 11.84TGQAASPRR54 pKa = 11.84IHH56 pKa = 6.27NVTRR60 pKa = 11.84IVTSFGLTPHH70 pKa = 5.77QHH72 pKa = 6.4DD73 pKa = 4.69LQRR76 pKa = 11.84GWTFDD81 pKa = 3.6PARR84 pKa = 11.84LAEE87 pKa = 4.1RR88 pKa = 11.84WRR90 pKa = 11.84RR91 pKa = 11.84LPWTDD96 pKa = 2.79

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

18030

28

1415

237.2

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.753 ± 0.355

0.849 ± 0.142

6.118 ± 0.311

6.334 ± 0.378

2.429 ± 0.135

9.806 ± 0.299

2.019 ± 0.181

3.955 ± 0.346

2.768 ± 0.287

8.048 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.136

2.418 ± 0.155

6.173 ± 0.238

3.006 ± 0.202

7.543 ± 0.384

5.635 ± 0.414

7.299 ± 0.448

7.781 ± 0.212

2.058 ± 0.144

2.091 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski