Rhodococcus hoagii ATCC 33707

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; Rhodococcus hoagii

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9SXS0|E9SXS0_RHOHA Transcriptional regulator AraC family OS=Rhodococcus hoagii ATCC 33707 OX=525370 GN=HMPREF0724_11135 PE=4 SV=1
MM1 pKa = 7.54TEE3 pKa = 4.93HH4 pKa = 7.33DD5 pKa = 3.87TTDD8 pKa = 3.48QPLQLDD14 pKa = 3.98GAFAIATDD22 pKa = 3.83SRR24 pKa = 11.84EE25 pKa = 3.98QLHH28 pKa = 6.34ALSLRR33 pKa = 11.84TLTEE37 pKa = 4.09LLGSAPEE44 pKa = 3.9VDD46 pKa = 3.79EE47 pKa = 6.04DD48 pKa = 4.3GDD50 pKa = 3.68IAIPVHH56 pKa = 6.51GFGLYY61 pKa = 8.47VTVAEE66 pKa = 5.5DD67 pKa = 4.43GPQLHH72 pKa = 6.17VWASILSEE80 pKa = 4.39LGDD83 pKa = 3.47GAVAVQNLPEE93 pKa = 5.37LSAEE97 pKa = 4.01WPRR100 pKa = 11.84LRR102 pKa = 11.84FTIEE106 pKa = 4.19DD107 pKa = 3.37GHH109 pKa = 8.46LLVSTLVDD117 pKa = 4.05ADD119 pKa = 3.75PFAPQHH125 pKa = 6.42LINLVDD131 pKa = 5.08EE132 pKa = 4.44IHH134 pKa = 6.75EE135 pKa = 4.33LTHH138 pKa = 7.64DD139 pKa = 4.57LDD141 pKa = 5.87DD142 pKa = 5.55DD143 pKa = 4.18FAAKK147 pKa = 10.46LGGVLDD153 pKa = 4.91CDD155 pKa = 5.05ADD157 pKa = 4.11DD158 pKa = 4.79DD159 pKa = 5.09SYY161 pKa = 12.23AGGCGGCGDD170 pKa = 3.71NCACGGHH177 pKa = 5.91NAGDD181 pKa = 3.88LGEE184 pKa = 4.93TIPIGQPSNN193 pKa = 3.39

Molecular weight: 20.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9SX78|E9SX78_RHOHA Uncharacterized protein OS=Rhodococcus hoagii ATCC 33707 OX=525370 GN=HMPREF0724_10729 PE=4 SV=1
RR1 pKa = 7.34HH2 pKa = 6.75PLPRR6 pKa = 11.84LPLPRR11 pKa = 11.84RR12 pKa = 11.84PHH14 pKa = 6.16RR15 pKa = 11.84SRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84LRR22 pKa = 11.84LRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84PSPVHH31 pKa = 5.01WASRR35 pKa = 11.84PARR38 pKa = 11.84RR39 pKa = 11.84LPAARR44 pKa = 11.84SSSPRR49 pKa = 11.84QRR51 pKa = 11.84PHH53 pKa = 6.53RR54 pKa = 11.84SRR56 pKa = 11.84WGRR59 pKa = 11.84FRR61 pKa = 11.84VRR63 pKa = 11.84APPPPHH69 pKa = 5.72TFSWRR74 pKa = 11.84HH75 pKa = 3.45TRR77 pKa = 11.84FRR79 pKa = 11.84CGSPVV84 pKa = 2.78

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5029

0

5029

1603601

37

8934

318.9

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.023 ± 0.043

0.755 ± 0.012

6.493 ± 0.037

5.518 ± 0.032

3.001 ± 0.019

9.181 ± 0.035

2.096 ± 0.018

3.993 ± 0.028

2.026 ± 0.033

9.847 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.952 ± 0.016

1.982 ± 0.021

5.665 ± 0.028

2.667 ± 0.018

7.44 ± 0.043

5.532 ± 0.025

6.202 ± 0.031

9.215 ± 0.038

1.437 ± 0.013

1.975 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski