Methanobrevibacter curvatus

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166ALI0|A0A166ALI0_9EURY Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Methanobrevibacter curvatus OX=49547 GN=ubiE PE=4 SV=1
MM1 pKa = 7.48FKK3 pKa = 11.01NKK5 pKa = 9.99LFMLNVCILIFILSFSLLSGVYY27 pKa = 10.36ADD29 pKa = 4.5NFINNEE35 pKa = 3.94DD36 pKa = 3.65NSNVKK41 pKa = 10.3SLLSAQSVIFDD52 pKa = 4.07NPNNLNDD59 pKa = 3.83YY60 pKa = 11.21DD61 pKa = 4.41GVNSDD66 pKa = 3.65NYY68 pKa = 10.31FYY70 pKa = 11.0AYY72 pKa = 10.33AYY74 pKa = 10.59GDD76 pKa = 3.87DD77 pKa = 5.94DD78 pKa = 4.72EE79 pKa = 7.27DD80 pKa = 3.79EE81 pKa = 6.15DD82 pKa = 5.21YY83 pKa = 10.27DD84 pKa = 3.82TDD86 pKa = 3.88EE87 pKa = 6.45DD88 pKa = 3.84EE89 pKa = 6.97DD90 pKa = 5.03YY91 pKa = 10.29DD92 pKa = 3.86TDD94 pKa = 3.88EE95 pKa = 6.45DD96 pKa = 3.84EE97 pKa = 6.97DD98 pKa = 5.03YY99 pKa = 10.29DD100 pKa = 3.86TDD102 pKa = 3.88EE103 pKa = 6.45DD104 pKa = 3.84EE105 pKa = 6.97DD106 pKa = 5.03YY107 pKa = 10.29DD108 pKa = 3.86TDD110 pKa = 3.88EE111 pKa = 6.45DD112 pKa = 3.84EE113 pKa = 6.97DD114 pKa = 5.03YY115 pKa = 10.29DD116 pKa = 3.86TDD118 pKa = 4.09EE119 pKa = 6.43DD120 pKa = 3.98EE121 pKa = 6.54DD122 pKa = 4.87YY123 pKa = 11.52DD124 pKa = 5.42GDD126 pKa = 5.27GDD128 pKa = 5.97DD129 pKa = 5.69EE130 pKa = 6.22DD131 pKa = 5.93DD132 pKa = 3.97SPNYY136 pKa = 9.98YY137 pKa = 9.43EE138 pKa = 4.81WIWKK142 pKa = 9.97GEE144 pKa = 4.06TYY146 pKa = 10.61YY147 pKa = 10.38ISLDD151 pKa = 3.67EE152 pKa = 4.98FSLTDD157 pKa = 3.45EE158 pKa = 4.6EE159 pKa = 4.43LTEE162 pKa = 5.05LFTKK166 pKa = 10.12RR167 pKa = 11.84DD168 pKa = 3.32KK169 pKa = 11.53LMEE172 pKa = 4.98EE173 pKa = 4.17IEE175 pKa = 4.14NLQSKK180 pKa = 9.5IYY182 pKa = 10.68EE183 pKa = 4.01MDD185 pKa = 3.55LSRR188 pKa = 11.84NQDD191 pKa = 3.9LILIIDD197 pKa = 3.9EE198 pKa = 5.21LYY200 pKa = 11.12NSINIISNNTEE211 pKa = 3.93FNEE214 pKa = 4.73LLLNLKK220 pKa = 10.15NISLNDD226 pKa = 3.58TKK228 pKa = 11.0STFNEE233 pKa = 3.95LKK235 pKa = 10.41ILLGSIKK242 pKa = 10.23EE243 pKa = 4.36DD244 pKa = 3.74YY245 pKa = 8.56PTEE248 pKa = 4.12NFTKK252 pKa = 9.88IDD254 pKa = 3.95DD255 pKa = 5.02LISTIEE261 pKa = 3.82LMLNEE266 pKa = 4.91EE267 pKa = 4.09YY268 pKa = 10.88LKK270 pKa = 11.17YY271 pKa = 10.95EE272 pKa = 4.15NLTQEE277 pKa = 4.22LAEE280 pKa = 4.41KK281 pKa = 10.82LEE283 pKa = 3.96EE284 pKa = 4.36LYY286 pKa = 10.98EE287 pKa = 4.44LFDD290 pKa = 4.4EE291 pKa = 5.23YY292 pKa = 10.9PFLIQNTKK300 pKa = 9.32YY301 pKa = 10.67AAVDD305 pKa = 3.56KK306 pKa = 10.73TLGAYY311 pKa = 10.02AKK313 pKa = 9.88FEE315 pKa = 4.27KK316 pKa = 10.67VSYY319 pKa = 9.65TSNNNTLAEE328 pKa = 4.28MKK330 pKa = 8.11TTGLPHH336 pKa = 6.21FQLVLLVLLLSLLGANIRR354 pKa = 11.84RR355 pKa = 11.84KK356 pKa = 9.49PP357 pKa = 3.32

Molecular weight: 41.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166B6I0|A0A166B6I0_9EURY Uncharacterized protein OS=Methanobrevibacter curvatus OX=49547 GN=MBCUR_08180 PE=4 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84NKK5 pKa = 9.92PLAKK9 pKa = 10.08KK10 pKa = 10.26LRR12 pKa = 11.84LAKK15 pKa = 10.31AFKK18 pKa = 9.96QNRR21 pKa = 11.84RR22 pKa = 11.84VPLWAIAKK30 pKa = 6.96TNRR33 pKa = 11.84KK34 pKa = 8.6VKK36 pKa = 9.78YY37 pKa = 7.75SHH39 pKa = 7.32KK40 pKa = 8.67PRR42 pKa = 11.84HH43 pKa = 4.76WRR45 pKa = 11.84RR46 pKa = 11.84NSLKK50 pKa = 10.43VV51 pKa = 3.18

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1968

0

1968

566479

30

2483

287.8

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.938 ± 0.06

0.932 ± 0.026

5.714 ± 0.046

6.986 ± 0.082

4.621 ± 0.051

6.096 ± 0.063

1.561 ± 0.023

10.234 ± 0.061

9.621 ± 0.077

9.15 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.029

7.897 ± 0.124

3.006 ± 0.039

2.079 ± 0.028

2.864 ± 0.041

7.049 ± 0.074

4.991 ± 0.054

5.751 ± 0.065

0.644 ± 0.017

3.897 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski