Eptesicus serotinus papillomavirus 3
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8EG03|W8EG03_9PAPI Regulatory protein E2 OS=Eptesicus serotinus papillomavirus 3 OX=1464073 GN=E2 PE=3 SV=1
MM1 pKa = 7.6 ALLLRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.81 RR10 pKa = 11.84 DD11 pKa = 3.32 TVEE14 pKa = 3.62 NIYY17 pKa = 10.03 RR18 pKa = 11.84 HH19 pKa = 6.5 CIRR22 pKa = 11.84 FGTCPPDD29 pKa = 3.36 VKK31 pKa = 11.19 NKK33 pKa = 9.74 VEE35 pKa = 3.96 QSTVADD41 pKa = 4.54 KK42 pKa = 10.75 ILKK45 pKa = 8.34 YY46 pKa = 10.87 GSGAVYY52 pKa = 10.25 FGGLGIGTGSGRR64 pKa = 11.84 GSTGVSVGRR73 pKa = 11.84 EE74 pKa = 3.72 VPIRR78 pKa = 11.84 PFRR81 pKa = 11.84 PHH83 pKa = 6.9 GPIDD87 pKa = 3.61 TIGIEE92 pKa = 4.26 NIGPRR97 pKa = 11.84 EE98 pKa = 4.1 TPFAPPDD105 pKa = 3.67 RR106 pKa = 11.84 APVDD110 pKa = 3.36 VDD112 pKa = 3.89 LGTGHH117 pKa = 6.49 VEE119 pKa = 3.95 PVDD122 pKa = 3.72 PSIITPIDD130 pKa = 3.98 LPTDD134 pKa = 3.44 IPTSSVDD141 pKa = 3.32 EE142 pKa = 4.91 SIVVPTPEE150 pKa = 3.67 ITIGGDD156 pKa = 2.93 TTAIIEE162 pKa = 4.3 VTPNVPDD169 pKa = 3.24 NTIISRR175 pKa = 11.84 SQYY178 pKa = 10.57 NNPAFDD184 pKa = 3.29 ISLFNNSTAGEE195 pKa = 4.3 LSASDD200 pKa = 4.81 HH201 pKa = 5.87 ITVSGGGGSIIGGHH215 pKa = 5.97 FPLAEE220 pKa = 4.23 PEE222 pKa = 4.16 GEE224 pKa = 4.17 EE225 pKa = 3.73 IGLAEE230 pKa = 4.11 FTPGVSRR237 pKa = 11.84 FRR239 pKa = 11.84 PRR241 pKa = 11.84 EE242 pKa = 3.77 VADD245 pKa = 3.54 EE246 pKa = 4.72 AEE248 pKa = 4.32 TSFITSTPEE257 pKa = 3.37 GARR260 pKa = 11.84 VGRR263 pKa = 11.84 PTRR266 pKa = 11.84 VRR268 pKa = 11.84 PSLYY272 pKa = 9.7 NRR274 pKa = 11.84 RR275 pKa = 11.84 IEE277 pKa = 4.1 QVEE280 pKa = 4.15 VTDD283 pKa = 3.9 PDD285 pKa = 4.52 FIEE288 pKa = 4.57 RR289 pKa = 11.84 PGRR292 pKa = 11.84 LVEE295 pKa = 4.53 FGYY298 pKa = 11.05 VNPAFEE304 pKa = 4.71 GDD306 pKa = 2.87 ITEE309 pKa = 4.75 IFEE312 pKa = 4.2 QDD314 pKa = 3.4 LLEE317 pKa = 4.34 EE318 pKa = 4.26 ARR320 pKa = 11.84 AAPSSAFQDD329 pKa = 3.2 IVRR332 pKa = 11.84 VGRR335 pKa = 11.84 QQLSRR340 pKa = 11.84 ARR342 pKa = 11.84 SGLVRR347 pKa = 11.84 VSRR350 pKa = 11.84 LGQRR354 pKa = 11.84 GTIRR358 pKa = 11.84 TRR360 pKa = 11.84 SGVRR364 pKa = 11.84 IGSTTHH370 pKa = 6.92 FYY372 pKa = 10.53 MDD374 pKa = 5.21 LSPILPDD381 pKa = 3.0 EE382 pKa = 4.44 SLPIEE387 pKa = 4.36 LPGEE391 pKa = 4.02 QSMEE395 pKa = 3.77 ASIVQPLSEE404 pKa = 4.45 EE405 pKa = 4.1 GFEE408 pKa = 4.78 LVNLDD413 pKa = 3.67 EE414 pKa = 4.87 VSDD417 pKa = 4.07 VVPDD421 pKa = 3.73 EE422 pKa = 4.54 SLLDD426 pKa = 3.92 EE427 pKa = 4.49 YY428 pKa = 11.34 EE429 pKa = 3.91 EE430 pKa = 4.67 TEE432 pKa = 4.98 FIGSDD437 pKa = 3.48 LQLIIHH443 pKa = 7.15 DD444 pKa = 4.01 EE445 pKa = 4.67 DD446 pKa = 3.68 IGIEE450 pKa = 4.01 RR451 pKa = 11.84 DD452 pKa = 3.0 SDD454 pKa = 3.67 IVVDD458 pKa = 4.23 VPSGFIKK465 pKa = 9.81 KK466 pKa = 8.43 TGTLYY471 pKa = 10.45 PDD473 pKa = 3.16 IDD475 pKa = 4.04 YY476 pKa = 10.26 TGVDD480 pKa = 2.75 IDD482 pKa = 3.87 YY483 pKa = 10.54 DD484 pKa = 3.83 KK485 pKa = 10.84 THH487 pKa = 6.78 SGDD490 pKa = 3.51 AGDD493 pKa = 5.56 IIPADD498 pKa = 3.93 TPVITIDD505 pKa = 2.62 IWSSDD510 pKa = 3.75 FYY512 pKa = 11.21 LHH514 pKa = 7.26 PSLSRR519 pKa = 11.84 KK520 pKa = 8.83 RR521 pKa = 11.84 KK522 pKa = 8.13 RR523 pKa = 11.84 RR524 pKa = 11.84 RR525 pKa = 11.84 KK526 pKa = 9.52 FVFVYY531 pKa = 10.67
Molecular weight: 58.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.676
IPC2_protein 4.584
IPC_protein 4.546
Toseland 4.393
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.38
Rodwell 4.393
Grimsley 4.304
Solomon 4.495
Lehninger 4.457
Nozaki 4.609
DTASelect 4.787
Thurlkill 4.393
EMBOSS 4.393
Sillero 4.66
Patrickios 4.088
IPC_peptide 4.507
IPC2_peptide 4.66
IPC2.peptide.svr19 4.605
Protein with the highest isoelectric point:
>tr|W8EH60|W8EH60_9PAPI Replication protein E1 OS=Eptesicus serotinus papillomavirus 3 OX=1464073 GN=E1 PE=3 SV=1
MM1 pKa = 7.92 SEE3 pKa = 3.92 LQNRR7 pKa = 11.84 LDD9 pKa = 4.13 YY10 pKa = 11.1 IQDD13 pKa = 3.5 QILEE17 pKa = 4.31 LFEE20 pKa = 4.46 KK21 pKa = 10.73 QSTDD25 pKa = 3.74 LQDD28 pKa = 5.48 HH29 pKa = 5.27 EE30 pKa = 4.86 QFWLLTRR37 pKa = 11.84 KK38 pKa = 8.76 EE39 pKa = 3.85 QAILFLARR47 pKa = 11.84 QNNVRR52 pKa = 11.84 LSMPVPSLAASKK64 pKa = 10.63 ARR66 pKa = 11.84 ARR68 pKa = 11.84 HH69 pKa = 5.56 AIEE72 pKa = 3.78 MSLLIKK78 pKa = 10.48 SLSEE82 pKa = 3.85 SVYY85 pKa = 10.69 GRR87 pKa = 11.84 EE88 pKa = 3.87 AWTLQQVSRR97 pKa = 11.84 EE98 pKa = 3.97 RR99 pKa = 11.84 LLVAPEE105 pKa = 3.77 YY106 pKa = 9.86 TFKK109 pKa = 11.04 KK110 pKa = 9.21 GGRR113 pKa = 11.84 PVTVMFDD120 pKa = 3.8 DD121 pKa = 3.89 NHH123 pKa = 7.04 DD124 pKa = 3.61 NTAEE128 pKa = 3.96 YY129 pKa = 8.59 TAWDD133 pKa = 3.63 SIYY136 pKa = 10.81 FQDD139 pKa = 6.02 ADD141 pKa = 4.27 NEE143 pKa = 4.24 WHH145 pKa = 6.74 RR146 pKa = 11.84 SKK148 pKa = 11.53 GDD150 pKa = 2.95 VDD152 pKa = 3.88 MEE154 pKa = 4.27 GLFYY158 pKa = 10.67 RR159 pKa = 11.84 DD160 pKa = 3.16 NDD162 pKa = 3.36 GHH164 pKa = 6.96 KK165 pKa = 9.81 IYY167 pKa = 10.97 YY168 pKa = 9.61 IDD170 pKa = 3.89 FARR173 pKa = 11.84 EE174 pKa = 3.42 AARR177 pKa = 11.84 YY178 pKa = 9.06 GSTGKK183 pKa = 9.71 WSLLYY188 pKa = 10.85 NNNALASVDD197 pKa = 3.63 SPGYY201 pKa = 9.29 RR202 pKa = 11.84 DD203 pKa = 3.34 SSGEE207 pKa = 4.02 SSTDD211 pKa = 3.23 SSPLSARR218 pKa = 11.84 SATRR222 pKa = 11.84 PTEE225 pKa = 4.39 DD226 pKa = 3.09 RR227 pKa = 11.84 GSPQGSRR234 pKa = 11.84 RR235 pKa = 11.84 RR236 pKa = 11.84 PRR238 pKa = 11.84 LWVRR242 pKa = 11.84 PRR244 pKa = 11.84 SRR246 pKa = 11.84 TRR248 pKa = 11.84 SRR250 pKa = 11.84 SLSRR254 pKa = 11.84 SSGRR258 pKa = 11.84 SRR260 pKa = 11.84 SRR262 pKa = 11.84 SRR264 pKa = 11.84 SRR266 pKa = 11.84 SRR268 pKa = 11.84 GATRR272 pKa = 11.84 ASIQPAAGTRR282 pKa = 11.84 SRR284 pKa = 11.84 SRR286 pKa = 11.84 SRR288 pKa = 11.84 TRR290 pKa = 11.84 SRR292 pKa = 11.84 SRR294 pKa = 11.84 PRR296 pKa = 11.84 SRR298 pKa = 11.84 GSPVSPASPASLRR311 pKa = 11.84 GGRR314 pKa = 11.84 SPGSRR319 pKa = 11.84 RR320 pKa = 11.84 LGRR323 pKa = 11.84 SPRR326 pKa = 11.84 PRR328 pKa = 11.84 AVDD331 pKa = 3.51 PEE333 pKa = 4.25 QVGSSKK339 pKa = 10.58 RR340 pKa = 11.84 SVPAGPKK347 pKa = 9.57 RR348 pKa = 11.84 RR349 pKa = 11.84 LEE351 pKa = 4.09 TLLQEE356 pKa = 4.97 ARR358 pKa = 11.84 DD359 pKa = 4.02 PPGLLLTGPPNTLKK373 pKa = 10.7 CYY375 pKa = 10.21 RR376 pKa = 11.84 YY377 pKa = 9.86 SLKK380 pKa = 10.59 KK381 pKa = 9.71 NHH383 pKa = 6.5 SSLFSYY389 pKa = 10.6 ISTTWHH395 pKa = 4.27 WTEE398 pKa = 3.5 AKK400 pKa = 8.33 GTKK403 pKa = 9.56 RR404 pKa = 11.84 VGNGKK409 pKa = 8.27 MLIMFEE415 pKa = 4.15 DD416 pKa = 3.74 SEE418 pKa = 4.37 RR419 pKa = 11.84 RR420 pKa = 11.84 EE421 pKa = 4.1 WFQKK425 pKa = 9.95 IVSLPRR431 pKa = 11.84 TLSASKK437 pKa = 10.95 VSFAGLL443 pKa = 3.21
Molecular weight: 50.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.809
IPC_protein 10.891
Toseland 10.906
ProMoST 10.906
Dawson 10.979
Bjellqvist 10.804
Wikipedia 11.286
Rodwell 11.008
Grimsley 11.038
Solomon 11.228
Lehninger 11.169
Nozaki 10.891
DTASelect 10.804
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 10.687
IPC_peptide 11.228
IPC2_peptide 9.926
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2365
104
642
394.2
44.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.342 ± 0.935
1.734 ± 0.729
6.554 ± 0.569
6.427 ± 0.546
4.778 ± 0.743
6.3 ± 0.912
2.03 ± 0.213
5.159 ± 1.197
4.524 ± 0.939
8.584 ± 0.799
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.86 ± 0.469
4.017 ± 0.754
5.793 ± 1.032
3.975 ± 0.623
7.738 ± 1.517
8.33 ± 1.477
5.539 ± 0.405
6.342 ± 0.729
1.268 ± 0.342
2.706 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here