Lactonifactor longoviformis DSM 17459

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Lactonifactor; Lactonifactor longoviformis

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4380 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4SUR5|A0A1M4SUR5_9CLOT Uncharacterized protein OS=Lactonifactor longoviformis DSM 17459 OX=1122155 GN=SAMN02745158_00274 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.87MNKK5 pKa = 8.15TLSVLCMTVLFAGSLSGCGNDD26 pKa = 3.5GSSPEE31 pKa = 4.22EE32 pKa = 4.15GGSASKK38 pKa = 11.02GGTLNVLTWEE48 pKa = 4.67GDD50 pKa = 3.65VSDD53 pKa = 3.83EE54 pKa = 4.14TVADD58 pKa = 4.14FEE60 pKa = 4.52EE61 pKa = 5.39EE62 pKa = 4.21YY63 pKa = 11.17GVDD66 pKa = 3.57VNITYY71 pKa = 10.09VEE73 pKa = 4.12DD74 pKa = 3.83TNTILAKK81 pKa = 9.55MLQGNSEE88 pKa = 4.11YY89 pKa = 11.09DD90 pKa = 3.59VIDD93 pKa = 3.65IEE95 pKa = 4.39SAYY98 pKa = 10.0MKK100 pKa = 10.57SFVDD104 pKa = 4.33AEE106 pKa = 4.17LLAPLDD112 pKa = 3.61YY113 pKa = 11.43DD114 pKa = 5.13KK115 pKa = 10.66ITNYY119 pKa = 10.65EE120 pKa = 4.2NIDD123 pKa = 3.38PVYY126 pKa = 10.33VEE128 pKa = 4.6KK129 pKa = 10.94GAMGDD134 pKa = 3.5EE135 pKa = 4.25KK136 pKa = 10.59LTYY139 pKa = 9.94SLPITGPLYY148 pKa = 10.44TGVVINKK155 pKa = 6.13EE156 pKa = 4.22TCPIEE161 pKa = 5.16IDD163 pKa = 3.83SFEE166 pKa = 4.82DD167 pKa = 3.38LADD170 pKa = 4.16PALEE174 pKa = 4.17GQVWSTNATISLYY187 pKa = 10.93AGALKK192 pKa = 10.75ALGYY196 pKa = 10.2SPNSQDD202 pKa = 3.23EE203 pKa = 4.47KK204 pKa = 11.1EE205 pKa = 4.26LNEE208 pKa = 4.36AQALLQKK215 pKa = 10.33IKK217 pKa = 10.78PNIKK221 pKa = 10.32AFGASSISSLEE232 pKa = 3.93TGEE235 pKa = 4.66CSVAYY240 pKa = 9.21TYY242 pKa = 10.68DD243 pKa = 3.73YY244 pKa = 10.49NILMFDD250 pKa = 5.55DD251 pKa = 3.82KK252 pKa = 11.92ANWDD256 pKa = 3.47KK257 pKa = 11.61FEE259 pKa = 4.53IVPEE263 pKa = 4.24TTLGYY268 pKa = 8.86CQYY271 pKa = 8.71WTVAASSEE279 pKa = 4.14NQKK282 pKa = 10.27LANEE286 pKa = 5.24FINYY290 pKa = 7.46TYY292 pKa = 10.68SAEE295 pKa = 4.24SAIKK299 pKa = 10.28LLNEE303 pKa = 4.0WGGVPALKK311 pKa = 10.37RR312 pKa = 11.84EE313 pKa = 4.31LIEE316 pKa = 4.17EE317 pKa = 4.61GVAKK321 pKa = 10.39DD322 pKa = 3.61YY323 pKa = 11.4FEE325 pKa = 6.26NPMLQEE331 pKa = 4.39FADD334 pKa = 3.56MWPDD338 pKa = 3.35HH339 pKa = 6.96EE340 pKa = 4.62EE341 pKa = 3.95LSVSDD346 pKa = 4.02EE347 pKa = 3.78QTAIMDD353 pKa = 3.95TLYY356 pKa = 11.04NEE358 pKa = 4.34LMSGEE363 pKa = 4.13

Molecular weight: 40.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4X6S9|A0A1M4X6S9_9CLOT Uncharacterized protein OS=Lactonifactor longoviformis DSM 17459 OX=1122155 GN=SAMN02745158_01859 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.51GGRR28 pKa = 11.84KK29 pKa = 8.86VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4380

0

4380

1447804

12

4715

330.5

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.765 ± 0.039

1.556 ± 0.015

5.257 ± 0.031

7.71 ± 0.041

3.951 ± 0.026

7.674 ± 0.042

1.7 ± 0.017

7.198 ± 0.038

6.365 ± 0.03

9.243 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.137 ± 0.02

3.892 ± 0.022

3.544 ± 0.021

3.299 ± 0.023

4.651 ± 0.03

5.778 ± 0.027

5.377 ± 0.03

6.82 ± 0.029

1.004 ± 0.016

4.079 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski