Antarctic penguin virus A
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0KBV0|A0A1Y0KBV0_9MONO Phosphoprotein OS=Antarctic penguin virus A OX=2006072 PE=4 SV=1
MM1 pKa = 7.33 SSVFAEE7 pKa = 3.64 YY8 pKa = 10.93 DD9 pKa = 3.23 KK10 pKa = 11.26 FLEE13 pKa = 4.62 NQTHH17 pKa = 6.01 PPSRR21 pKa = 11.84 ALPTEE26 pKa = 4.44 GGGSLKK32 pKa = 10.74 VEE34 pKa = 4.0 VPVFVLNSDD43 pKa = 3.78 DD44 pKa = 4.6 PEE46 pKa = 4.24 LRR48 pKa = 11.84 WRR50 pKa = 11.84 FVCFCLRR57 pKa = 11.84 LAISEE62 pKa = 4.23 SSNRR66 pKa = 11.84 PLRR69 pKa = 11.84 QGALISVLCAHH80 pKa = 6.56 AQVMKK85 pKa = 10.27 TYY87 pKa = 9.07 ATLAAQSGEE96 pKa = 3.92 AVITILEE103 pKa = 4.32 IDD105 pKa = 4.38 DD106 pKa = 5.65 FDD108 pKa = 6.77 DD109 pKa = 4.4 KK110 pKa = 11.44 MPVFNSRR117 pKa = 11.84 SGITDD122 pKa = 2.93 EE123 pKa = 4.27 RR124 pKa = 11.84 AARR127 pKa = 11.84 LALIAADD134 pKa = 3.67 IPRR137 pKa = 11.84 ACSNEE142 pKa = 4.11 TPFANAATEE151 pKa = 4.03 NEE153 pKa = 4.21 PPEE156 pKa = 4.66 DD157 pKa = 4.57 ASDD160 pKa = 3.45 TLDD163 pKa = 4.1 RR164 pKa = 11.84 IFSVQVQVWITVAKK178 pKa = 10.69 AMTAFEE184 pKa = 4.14 TAEE187 pKa = 3.87 EE188 pKa = 4.62 SEE190 pKa = 4.11 TRR192 pKa = 11.84 RR193 pKa = 11.84 LNKK196 pKa = 9.94 YY197 pKa = 6.6 MQQGRR202 pKa = 11.84 VQKK205 pKa = 10.59 KK206 pKa = 8.44 CLLYY210 pKa = 10.01 PIVRR214 pKa = 11.84 TTVQMTIRR222 pKa = 11.84 QSLVIRR228 pKa = 11.84 GFLVNEE234 pKa = 4.39 MKK236 pKa = 10.63 RR237 pKa = 11.84 AQNSPGGKK245 pKa = 7.92 SAYY248 pKa = 9.76 YY249 pKa = 10.5 SFVGDD254 pKa = 3.49 ISAYY258 pKa = 9.24 IKK260 pKa = 10.75 NAGVTAFLLTLKK272 pKa = 10.75 FGIQTRR278 pKa = 11.84 LPALALSSLAGDD290 pKa = 3.52 IQKK293 pKa = 10.13 VKK295 pKa = 10.86 QLMVLYY301 pKa = 10.29 RR302 pKa = 11.84 EE303 pKa = 4.35 KK304 pKa = 11.31 GEE306 pKa = 4.38 NGPFMTLLGDD316 pKa = 3.88 PDD318 pKa = 3.59 QMQFAPAEE326 pKa = 4.01 YY327 pKa = 10.29 SLLYY331 pKa = 10.23 SYY333 pKa = 11.85 AMGVASVLEE342 pKa = 4.46 ASTSRR347 pKa = 11.84 YY348 pKa = 8.5 QFARR352 pKa = 11.84 DD353 pKa = 3.51 FMNPTFWRR361 pKa = 11.84 IGVEE365 pKa = 4.3 SAQSLSTSVDD375 pKa = 2.96 EE376 pKa = 5.23 QMATEE381 pKa = 4.25 LQLGRR386 pKa = 11.84 QSRR389 pKa = 11.84 AALGEE394 pKa = 3.94 MMAKK398 pKa = 9.69 VAGSAGEE405 pKa = 4.06 YY406 pKa = 9.77 TMNAPAAAVMIGASGPNPPSSARR429 pKa = 11.84 QSGSGPKK436 pKa = 8.23 QTEE439 pKa = 3.85 SDD441 pKa = 3.26 IQLPPGFNTPEE452 pKa = 4.78 DD453 pKa = 3.58 YY454 pKa = 10.89 LAYY457 pKa = 9.73 IRR459 pKa = 11.84 NEE461 pKa = 3.86 EE462 pKa = 3.98 AFKK465 pKa = 10.81 SGRR468 pKa = 11.84 ATSSTEE474 pKa = 3.91 VPKK477 pKa = 10.8 TPGLAGDD484 pKa = 4.77 NDD486 pKa = 4.1 PQVDD490 pKa = 3.58 WEE492 pKa = 4.29 LL493 pKa = 4.01
Molecular weight: 53.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.075
IPC2_protein 5.143
IPC_protein 5.067
Toseland 4.94
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.143
Wikipedia 4.851
Rodwell 4.914
Grimsley 4.851
Solomon 5.003
Lehninger 4.952
Nozaki 5.105
DTASelect 5.232
Thurlkill 4.927
EMBOSS 4.876
Sillero 5.181
Patrickios 4.19
IPC_peptide 5.016
IPC2_peptide 5.181
IPC2.peptide.svr19 5.187
Protein with the highest isoelectric point:
>tr|A0A1Y0KBW5|A0A1Y0KBW5_9MONO Hemagglutinin-neuraminidase OS=Antarctic penguin virus A OX=2006072 PE=3 SV=1
MM1 pKa = 7.51 LRR3 pKa = 11.84 AEE5 pKa = 4.79 DD6 pKa = 3.68 LASRR10 pKa = 11.84 PIIGQQPAISMEE22 pKa = 3.84 SRR24 pKa = 11.84 TVKK27 pKa = 10.6 LYY29 pKa = 11.02 VDD31 pKa = 3.97 PDD33 pKa = 3.76 EE34 pKa = 4.99 PASSLLAFPLIFTSTSDD51 pKa = 3.27 GKK53 pKa = 9.75 KK54 pKa = 8.33 TLSPQYY60 pKa = 9.81 RR61 pKa = 11.84 IQIIDD66 pKa = 3.7 GEE68 pKa = 4.42 RR69 pKa = 11.84 EE70 pKa = 3.9 SDD72 pKa = 3.22 SDD74 pKa = 4.76 LIFISTYY81 pKa = 10.5 GFISGMEE88 pKa = 4.01 TAVDD92 pKa = 3.29 KK93 pKa = 11.38 SLAVDD98 pKa = 3.77 MGQEE102 pKa = 3.99 RR103 pKa = 11.84 TILTSCMLPLGSIPKK118 pKa = 9.82 VPDD121 pKa = 3.08 FHH123 pKa = 8.33 GLAKK127 pKa = 10.39 SCLEE131 pKa = 4.39 LKK133 pKa = 10.52 VSCKK137 pKa = 10.03 KK138 pKa = 10.51 AATNSEE144 pKa = 4.1 RR145 pKa = 11.84 IIFNVTDD152 pKa = 4.48 FPPILAQCAAIKK164 pKa = 10.55 KK165 pKa = 10.07 GVTSCNASVNLKK177 pKa = 10.49 APEE180 pKa = 4.83 KK181 pKa = 10.88 IMGNYY186 pKa = 9.91 DD187 pKa = 3.49 LVYY190 pKa = 10.49 KK191 pKa = 9.23 VTFVSLTVIPASLVYY206 pKa = 10.18 KK207 pKa = 10.52 VSSPVLKK214 pKa = 10.57 AGSSLTYY221 pKa = 9.72 SLNMSVIIKK230 pKa = 10.25 VDD232 pKa = 3.02 ITDD235 pKa = 3.44 KK236 pKa = 11.2 HH237 pKa = 6.08 PSAKK241 pKa = 10.39 LLLKK245 pKa = 10.44 KK246 pKa = 10.41 DD247 pKa = 3.8 DD248 pKa = 3.85 QYY250 pKa = 12.08 LANLWVHH257 pKa = 6.71 WGLISAVKK265 pKa = 10.37 KK266 pKa = 9.72 GGKK269 pKa = 8.63 RR270 pKa = 11.84 HH271 pKa = 5.76 TIEE274 pKa = 3.9 EE275 pKa = 4.17 VAEE278 pKa = 4.34 KK279 pKa = 10.24 IRR281 pKa = 11.84 RR282 pKa = 11.84 LDD284 pKa = 3.4 IKK286 pKa = 10.78 IEE288 pKa = 3.94 LVDD291 pKa = 4.2 LFGPSLIIQCKK302 pKa = 7.99 GVKK305 pKa = 8.84 TKK307 pKa = 10.8 LLAGFFSKK315 pKa = 10.38 QGTAVYY321 pKa = 8.82 PISRR325 pKa = 11.84 AAPQIGKK332 pKa = 9.15 LLWSQTGTIMEE343 pKa = 4.57 ASVVIQGGNQTQLASTSDD361 pKa = 3.79 YY362 pKa = 10.95 VIEE365 pKa = 4.29 STKK368 pKa = 9.71 VTLGKK373 pKa = 10.81 GNSKK377 pKa = 10.61 YY378 pKa = 11.05 NPFRR382 pKa = 11.84 KK383 pKa = 9.75
Molecular weight: 41.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.699
IPC2_protein 8.551
IPC_protein 8.419
Toseland 9.238
ProMoST 9.048
Dawson 9.502
Bjellqvist 9.268
Wikipedia 9.633
Rodwell 9.853
Grimsley 9.531
Solomon 9.545
Lehninger 9.516
Nozaki 9.458
DTASelect 9.18
Thurlkill 9.37
EMBOSS 9.677
Sillero 9.487
Patrickios 4.991
IPC_peptide 9.545
IPC2_peptide 7.834
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4620
383
2202
770.0
84.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.835 ± 1.107
1.991 ± 0.372
5.173 ± 0.49
4.156 ± 0.417
3.247 ± 0.383
5.952 ± 0.513
1.797 ± 0.37
7.662 ± 0.72
4.372 ± 0.538
10.584 ± 0.879
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.373
4.567 ± 0.345
4.87 ± 0.54
5.065 ± 0.514
5.043 ± 0.63
8.918 ± 0.525
6.212 ± 0.511
5.931 ± 0.438
0.844 ± 0.179
3.247 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here