Desulfovibrio sp. FSS-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; unclassified Desulfovibrio

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3975 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6V8LS19|A0A6V8LS19_9DELT Uncharacterized protein OS=Desulfovibrio sp. FSS-1 OX=2730080 GN=NNJEOMEG_00411 PE=4 SV=1
MM1 pKa = 7.53ALDD4 pKa = 4.08EE5 pKa = 5.41PKK7 pKa = 10.96DD8 pKa = 3.65SDD10 pKa = 5.35DD11 pKa = 3.59VFEE14 pKa = 4.3TDD16 pKa = 3.86GYY18 pKa = 11.42SFIIDD23 pKa = 3.62KK24 pKa = 10.82EE25 pKa = 4.35LMGKK29 pKa = 8.61AQPVTVDD36 pKa = 3.2LSYY39 pKa = 10.83MGFQVHH45 pKa = 6.62SSLEE49 pKa = 4.12LGGGGCGSSCSSGSCGQQ66 pKa = 3.25

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6V8LKG8|A0A6V8LKG8_9DELT Sensory transduction protein LytR OS=Desulfovibrio sp. FSS-1 OX=2730080 GN=lytR_1 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.84QPHH9 pKa = 4.42NTRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.56RR15 pKa = 11.84THH17 pKa = 5.83GFLVRR22 pKa = 11.84SRR24 pKa = 11.84TKK26 pKa = 10.16NGRR29 pKa = 11.84AIIRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.67GRR40 pKa = 11.84KK41 pKa = 8.98RR42 pKa = 11.84LGAA45 pKa = 3.98

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3975

0

3975

1316110

29

2874

331.1

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.254 ± 0.061

1.294 ± 0.015

5.235 ± 0.029

6.147 ± 0.038

3.846 ± 0.024

8.536 ± 0.037

2.063 ± 0.021

3.842 ± 0.037

3.697 ± 0.038

11.445 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.017

2.402 ± 0.023

5.504 ± 0.037

3.215 ± 0.027

7.328 ± 0.044

5.225 ± 0.03

4.567 ± 0.026

7.384 ± 0.028

1.266 ± 0.017

2.265 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski