Staphylococcus phage K

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Kayvirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6Y7U4|Q6Y7U4_BPPGK ORF22 OS=Staphylococcus phage K OX=221915 GN=PhageK_060 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.26KK3 pKa = 9.73INSVIKK9 pKa = 10.92GEE11 pKa = 4.2GKK13 pKa = 10.02KK14 pKa = 10.08VQTADD19 pKa = 3.12VRR21 pKa = 11.84KK22 pKa = 9.23ISYY25 pKa = 8.66YY26 pKa = 10.15VKK28 pKa = 10.35DD29 pKa = 4.36YY30 pKa = 10.2NPCMTVDD37 pKa = 4.45DD38 pKa = 5.09ANDD41 pKa = 3.4YY42 pKa = 10.76NATSQYY48 pKa = 10.96LVSDD52 pKa = 3.42NGKK55 pKa = 10.45FIAKK59 pKa = 9.62YY60 pKa = 9.38NKK62 pKa = 10.09DD63 pKa = 3.24MNAVGFYY70 pKa = 10.07EE71 pKa = 4.24EE72 pKa = 4.91SGDD75 pKa = 4.03TVKK78 pKa = 10.75HH79 pKa = 5.35LTHH82 pKa = 6.44TTPEE86 pKa = 4.1RR87 pKa = 11.84LEE89 pKa = 4.14GTVFTIEE96 pKa = 4.26EE97 pKa = 4.15EE98 pKa = 4.52TEE100 pKa = 3.37IDD102 pKa = 5.22LINDD106 pKa = 4.19TLPQGDD112 pKa = 4.13ILIKK116 pKa = 10.75FSDD119 pKa = 3.82GSIYY123 pKa = 10.81LPDD126 pKa = 3.99NEE128 pKa = 4.94SVLDD132 pKa = 3.97SVNYY136 pKa = 10.3LADD139 pKa = 4.14NDD141 pKa = 4.11WDD143 pKa = 4.03SVDD146 pKa = 4.77DD147 pKa = 4.66IIYY150 pKa = 9.68TGLSKK155 pKa = 11.1GNSEE159 pKa = 3.98NCIVDD164 pKa = 4.23FNYY167 pKa = 10.51NNYY170 pKa = 10.39DD171 pKa = 2.83IGYY174 pKa = 10.04DD175 pKa = 3.6DD176 pKa = 5.84VEE178 pKa = 6.23DD179 pKa = 5.06EE180 pKa = 4.88DD181 pKa = 5.07VCDD184 pKa = 4.64NYY186 pKa = 10.78PEE188 pKa = 4.77CEE190 pKa = 4.02CSNYY194 pKa = 9.78CSSTGEE200 pKa = 4.12YY201 pKa = 9.67IGNN204 pKa = 3.72

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6Y7S1|Q6Y7S1_BPPGK ORF46 OS=Staphylococcus phage K OX=221915 GN=PhageK_096 PE=4 SV=1
MM1 pKa = 7.55PVHH4 pKa = 6.96GKK6 pKa = 9.93RR7 pKa = 11.84PNLFKK12 pKa = 11.06NKK14 pKa = 9.6NYY16 pKa = 9.97KK17 pKa = 9.74QVGKK21 pKa = 8.94RR22 pKa = 11.84TIDD25 pKa = 3.47GMRR28 pKa = 11.84SEE30 pKa = 5.19VLDD33 pKa = 3.88KK34 pKa = 11.28LQATAQQVEE43 pKa = 4.3NTSIKK48 pKa = 10.49RR49 pKa = 11.84MPTYY53 pKa = 10.71LQITEE58 pKa = 4.38KK59 pKa = 10.63KK60 pKa = 9.56LEE62 pKa = 4.11KK63 pKa = 10.69EE64 pKa = 4.25GVVDD68 pKa = 4.32LKK70 pKa = 11.14KK71 pKa = 10.96AFAHH75 pKa = 5.82SSKK78 pKa = 10.89KK79 pKa = 9.12KK80 pKa = 7.77TSKK83 pKa = 10.92DD84 pKa = 3.25GGWYY88 pKa = 8.16LTVPIRR94 pKa = 11.84IKK96 pKa = 9.49TSRR99 pKa = 11.84MNNSTYY105 pKa = 10.9QDD107 pKa = 3.27MRR109 pKa = 11.84TLKK112 pKa = 10.02VDD114 pKa = 2.98KK115 pKa = 10.28GTGSVSKK122 pKa = 9.69ITDD125 pKa = 3.43YY126 pKa = 11.77LEE128 pKa = 4.1GRR130 pKa = 11.84RR131 pKa = 11.84KK132 pKa = 9.67NVSHH136 pKa = 7.49PSMKK140 pKa = 9.91PEE142 pKa = 4.04PMTHH146 pKa = 6.11NMTKK150 pKa = 10.14VKK152 pKa = 10.24RR153 pKa = 11.84GKK155 pKa = 9.51QSSYY159 pKa = 10.61FIFRR163 pKa = 11.84TVSSKK168 pKa = 11.09SPASSWILNRR178 pKa = 11.84DD179 pKa = 3.18KK180 pKa = 11.54VNEE183 pKa = 4.21DD184 pKa = 3.28NFSKK188 pKa = 8.25TTLKK192 pKa = 9.76TVKK195 pKa = 10.24QLMNWKK201 pKa = 8.72MKK203 pKa = 10.32NLNN206 pKa = 3.45

Molecular weight: 23.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

32918

99

1351

286.2

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.347 ± 0.273

0.495 ± 0.063

7.233 ± 0.163

8.084 ± 0.282

3.907 ± 0.126

6.109 ± 0.424

1.586 ± 0.101

7.066 ± 0.236

9.068 ± 0.255

7.804 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.139

6.708 ± 0.151

2.953 ± 0.209

3.758 ± 0.181

3.618 ± 0.117

6.799 ± 0.198

5.951 ± 0.267

6.279 ± 0.222

0.832 ± 0.069

5.006 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski