Acinetobacter phage YMC/09/02/B1251

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vieuvirus; Acinetobacter virus B1251

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7I460|J7I460_9CAUD Terminase_6C domain-containing protein OS=Acinetobacter phage YMC/09/02/B1251 OX=1221835 GN=BPABA456_00200 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.83NTWHH6 pKa = 7.41ADD8 pKa = 3.27QEE10 pKa = 4.57KK11 pKa = 10.67PEE13 pKa = 4.41LRR15 pKa = 11.84PDD17 pKa = 3.96EE18 pKa = 5.24KK19 pKa = 10.88PLNCPFCGSDD29 pKa = 4.49SICTDD34 pKa = 2.7SSHH37 pKa = 6.26YY38 pKa = 10.56GKK40 pKa = 9.59PDD42 pKa = 3.37EE43 pKa = 5.6DD44 pKa = 5.62GSIAWDD50 pKa = 3.33AFTWCHH56 pKa = 6.28DD57 pKa = 4.29CGSKK61 pKa = 10.94GPSAWAMIAWDD72 pKa = 4.14EE73 pKa = 4.12NFHH76 pKa = 6.39YY77 pKa = 9.37DD78 pKa = 2.95TVYY81 pKa = 10.77EE82 pKa = 3.99EE83 pKa = 4.22RR84 pKa = 11.84SIVNYY89 pKa = 10.61AIRR92 pKa = 11.84QWNTRR97 pKa = 11.84KK98 pKa = 10.12

Molecular weight: 11.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7I452|J7I452_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC/09/02/B1251 OX=1221835 GN=BPABA456_00050 PE=4 SV=1
MM1 pKa = 7.08THH3 pKa = 6.78FKK5 pKa = 10.28KK6 pKa = 10.2HH7 pKa = 5.75PDD9 pKa = 3.37GYY11 pKa = 11.13KK12 pKa = 10.38SFLGRR17 pKa = 11.84DD18 pKa = 3.51DD19 pKa = 3.9KK20 pKa = 11.62GLYY23 pKa = 8.82SVRR26 pKa = 11.84IGWQVYY32 pKa = 9.57ASNANGSVLYY42 pKa = 10.15KK43 pKa = 10.84VKK45 pKa = 10.89DD46 pKa = 3.73GFKK49 pKa = 10.39TPLNVYY55 pKa = 9.62KK56 pKa = 10.69FQTDD60 pKa = 3.81YY61 pKa = 11.27PKK63 pKa = 10.78VWNEE67 pKa = 3.43LTQEE71 pKa = 3.45IDD73 pKa = 3.47FQRR76 pKa = 11.84RR77 pKa = 11.84KK78 pKa = 9.79QLAIKK83 pKa = 10.26LRR85 pKa = 11.84EE86 pKa = 4.24TNIPTYY92 pKa = 10.38DD93 pKa = 3.01RR94 pKa = 11.84KK95 pKa = 10.61AYY97 pKa = 7.93KK98 pKa = 9.58QKK100 pKa = 10.84RR101 pKa = 11.84GFTGSRR107 pKa = 3.24

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13620

49

1631

219.7

24.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.128 ± 0.67

0.932 ± 0.179

5.852 ± 0.197

7.247 ± 0.367

4.046 ± 0.234

6.329 ± 0.416

1.645 ± 0.187

6.101 ± 0.279

7.856 ± 0.424

8.737 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.132

5.499 ± 0.326

3.612 ± 0.345

4.648 ± 0.414

4.427 ± 0.186

6.16 ± 0.366

5.551 ± 0.373

6.329 ± 0.266

1.131 ± 0.133

3.539 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski