Feline herpesvirus 1 (FeHV-1) (Feline viral rhinotracheitis virus)
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1FXW3|D1FXW3_FHV1 Major capsid protein OS=Feline herpesvirus 1 OX=10334 GN=UL19 PE=2 SV=1
MM1 pKa = 7.39 ATLCRR6 pKa = 11.84 DD7 pKa = 3.55 DD8 pKa = 4.58 DD9 pKa = 3.64 QAGIIIHH16 pKa = 6.25 DD17 pKa = 4.14 QEE19 pKa = 5.55 LDD21 pKa = 3.52 SDD23 pKa = 4.32 DD24 pKa = 5.02 EE25 pKa = 4.8 SVCEE29 pKa = 4.17 YY30 pKa = 10.71 ISMSAYY36 pKa = 10.45 GGDD39 pKa = 3.77 ADD41 pKa = 4.8 FLLSSAYY48 pKa = 9.31 TIEE51 pKa = 4.56 PGKK54 pKa = 9.69 HH55 pKa = 4.59 QPTFTISVVFFAISAFVIKK74 pKa = 9.32 PLCCLAFLNYY84 pKa = 9.66 YY85 pKa = 9.89 RR86 pKa = 11.84 ATGNSIFITSGGIITLVYY104 pKa = 9.51 YY105 pKa = 9.3 IQSMLMIFFIYY116 pKa = 10.67 NNIRR120 pKa = 11.84 VDD122 pKa = 4.36 LLPLRR127 pKa = 11.84 WWQQAVIGSMSVGRR141 pKa = 11.84 LIAFIVIFHH150 pKa = 6.09 SAIFVDD156 pKa = 3.93 SEE158 pKa = 4.31 LFFRR162 pKa = 11.84 VSSSDD167 pKa = 3.18 SPMDD171 pKa = 3.95 YY172 pKa = 9.11 ITPLIVHH179 pKa = 6.51 TLFPSISVRR188 pKa = 11.84 TVAFMLIIAAAVYY201 pKa = 10.1 VADD204 pKa = 5.69 AICDD208 pKa = 3.43 AVEE211 pKa = 4.96 FVAPRR216 pKa = 11.84 MWVCILMRR224 pKa = 11.84 TRR226 pKa = 11.84 LSFF229 pKa = 3.81
Molecular weight: 25.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.454
IPC2_protein 4.596
IPC_protein 4.533
Toseland 4.355
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.673
Wikipedia 4.457
Rodwell 4.38
Grimsley 4.266
Solomon 4.52
Lehninger 4.482
Nozaki 4.647
DTASelect 4.902
Thurlkill 4.393
EMBOSS 4.482
Sillero 4.673
Patrickios 0.757
IPC_peptide 4.52
IPC2_peptide 4.66
IPC2.peptide.svr19 4.545
Protein with the highest isoelectric point:
>tr|D1FXS3|D1FXS3_FHV1 Membrane protein UL56 OS=Feline herpesvirus 1 OX=10334 GN=UL56 PE=2 SV=1
MM1 pKa = 6.62 TRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 VLAPRR10 pKa = 11.84 EE11 pKa = 3.86 LEE13 pKa = 3.84 AARR16 pKa = 11.84 KK17 pKa = 9.13 LRR19 pKa = 11.84 EE20 pKa = 4.08 IFNAEE25 pKa = 3.8 YY26 pKa = 10.41 VAPTFTLVDD35 pKa = 4.14 PGDD38 pKa = 3.5 TSNAYY43 pKa = 8.8 IVCRR47 pKa = 11.84 TPVTEE52 pKa = 4.25 VVSSISRR59 pKa = 11.84 GIDD62 pKa = 2.93 NRR64 pKa = 11.84 KK65 pKa = 9.78 SVDD68 pKa = 3.11 SSFIRR73 pKa = 11.84 IVSKK77 pKa = 10.64 LIIRR81 pKa = 11.84 NAIHH85 pKa = 6.31 MGLSVLCAFISYY97 pKa = 9.68 NKK99 pKa = 9.5 PP100 pKa = 3.0
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.245
IPC2_protein 9.253
IPC_protein 9.56
Toseland 9.926
ProMoST 9.721
Dawson 10.16
Bjellqvist 9.911
Wikipedia 10.365
Rodwell 10.365
Grimsley 10.248
Solomon 10.233
Lehninger 10.204
Nozaki 10.014
DTASelect 9.882
Thurlkill 10.014
EMBOSS 10.35
Sillero 10.101
Patrickios 10.028
IPC_peptide 10.233
IPC2_peptide 8.946
IPC2.peptide.svr19 8.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
37492
76
3033
513.6
57.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.663 ± 0.203
1.947 ± 0.12
5.791 ± 0.17
5.054 ± 0.149
3.889 ± 0.175
6.124 ± 0.225
2.675 ± 0.097
6.684 ± 0.189
3.473 ± 0.19
9.781 ± 0.213
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.111
4.158 ± 0.155
5.796 ± 0.319
3.129 ± 0.116
6.681 ± 0.185
8.14 ± 0.22
7.271 ± 0.212
6.023 ± 0.116
0.968 ± 0.06
3.395 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here