Vagococcus penaei

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2D746|A0A1Q2D746_9ENTE Phosphonate C-P lyase system protein PhnL OS=Vagococcus penaei OX=633807 GN=BW732_08200 PE=4 SV=1
MM1 pKa = 6.67THH3 pKa = 5.66EE4 pKa = 5.03HH5 pKa = 5.84NHH7 pKa = 5.88DD8 pKa = 3.87HH9 pKa = 7.08DD10 pKa = 4.96HH11 pKa = 6.79EE12 pKa = 6.36HH13 pKa = 6.97DD14 pKa = 3.81QDD16 pKa = 4.43DD17 pKa = 5.85LITLVDD23 pKa = 3.8DD24 pKa = 4.63EE25 pKa = 4.84GNEE28 pKa = 3.91VLFRR32 pKa = 11.84IHH34 pKa = 6.3LTIDD38 pKa = 3.19GQEE41 pKa = 4.08QFGKK45 pKa = 10.41DD46 pKa = 3.49YY47 pKa = 11.45VLLYY51 pKa = 10.6DD52 pKa = 4.08ASTPEE57 pKa = 4.13GEE59 pKa = 4.42EE60 pKa = 4.23VEE62 pKa = 4.36LLAYY66 pKa = 10.04AYY68 pKa = 8.78EE69 pKa = 4.17QVEE72 pKa = 4.24GDD74 pKa = 4.13DD75 pKa = 4.28EE76 pKa = 4.55GHH78 pKa = 6.41LMEE81 pKa = 5.76VEE83 pKa = 4.8TEE85 pKa = 4.28EE86 pKa = 4.12EE87 pKa = 3.96WNMIEE92 pKa = 4.11EE93 pKa = 4.27VFNAFEE99 pKa = 4.29EE100 pKa = 4.53EE101 pKa = 4.41GEE103 pKa = 4.26EE104 pKa = 4.14

Molecular weight: 12.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2D651|A0A1Q2D651_9ENTE Methyltransf_25 domain-containing protein OS=Vagococcus penaei OX=633807 GN=BW732_06475 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2060

0

2060

654354

29

1791

317.6

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.744 ± 0.057

0.677 ± 0.015

5.614 ± 0.037

6.621 ± 0.058

4.391 ± 0.046

6.446 ± 0.048

1.917 ± 0.024

7.87 ± 0.052

6.796 ± 0.05

10.13 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.024

4.671 ± 0.038

3.451 ± 0.028

4.27 ± 0.044

3.71 ± 0.043

6.095 ± 0.037

6.3 ± 0.043

7.039 ± 0.046

0.844 ± 0.019

3.669 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski