Mitsuaria sp. PDC51

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Mitsuaria; unclassified Mitsuaria

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6KPA0|A0A1I6KPA0_9BURK Cobalt-zinc-cadmium resistance protein CzcA OS=Mitsuaria sp. PDC51 OX=1881035 GN=SAMN05428960_3342 PE=3 SV=1
MM1 pKa = 7.62TNTFYY6 pKa = 11.24VAAQVSADD14 pKa = 4.03GNFADD19 pKa = 4.02CTYY22 pKa = 10.84YY23 pKa = 11.1ADD25 pKa = 3.76QAGTEE30 pKa = 4.34PIEE33 pKa = 4.48GSTLHH38 pKa = 6.44IPRR41 pKa = 11.84DD42 pKa = 3.54AGSCNFAQADD52 pKa = 3.94NTSLLLIGATFKK64 pKa = 10.72TIGSTPGMNGSNFAPADD81 pKa = 3.79DD82 pKa = 4.46EE83 pKa = 4.72NVVTFPMPTNGSIITKK99 pKa = 10.05GVILLFSTPGQVEE112 pKa = 4.01NLYY115 pKa = 10.22PSSDD119 pKa = 3.57PQVLNDD125 pKa = 5.16GPLTCC130 pKa = 6.03

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6KTF2|A0A1I6KTF2_9BURK Uncharacterized protein OS=Mitsuaria sp. PDC51 OX=1881035 GN=SAMN05428960_3741 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.39RR3 pKa = 11.84TYY5 pKa = 10.12QGSKK9 pKa = 6.25TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.94TRR25 pKa = 11.84GGKK28 pKa = 9.68AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4932

0

4932

1770870

29

5536

359.1

38.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.605 ± 0.055

0.793 ± 0.01

5.527 ± 0.024

5.225 ± 0.036

3.222 ± 0.022

8.571 ± 0.041

2.145 ± 0.017

3.773 ± 0.022

2.829 ± 0.031

11.091 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.016

2.308 ± 0.027

5.652 ± 0.032

3.999 ± 0.026

7.625 ± 0.043

5.327 ± 0.035

5.12 ± 0.037

7.308 ± 0.028

1.571 ± 0.017

2.028 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski