Comamonas terrigena
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4009 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A7USC3|A0A2A7USC3_COMTR TRAP transporter small permease OS=Comamonas terrigena OX=32013 GN=CRM82_05725 PE=4 SV=1
MM1 pKa = 7.26 SAVAEE6 pKa = 4.12 NTITEE11 pKa = 4.29 MPAPILFTDD20 pKa = 3.95 SAAAKK25 pKa = 10.02 VADD28 pKa = 5.45 LIAEE32 pKa = 4.31 EE33 pKa = 4.82 GNPDD37 pKa = 2.99 LKK39 pKa = 11.13 LRR41 pKa = 11.84 VFVQGGGCSGFQYY54 pKa = 10.94 GFTFDD59 pKa = 5.34 EE60 pKa = 4.5 ITNDD64 pKa = 4.71 DD65 pKa = 4.31 DD66 pKa = 3.88 TTMTKK71 pKa = 10.62 NGVSLLIDD79 pKa = 3.54 AMSYY83 pKa = 10.32 QYY85 pKa = 11.5 LVGAEE90 pKa = 3.79 IDD92 pKa = 3.91 YY93 pKa = 11.36 KK94 pKa = 11.19 EE95 pKa = 4.67 DD96 pKa = 3.23 LQGAQFVIKK105 pKa = 10.36 NPNASTTCGCGSSFSVV121 pKa = 3.54
Molecular weight: 12.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A2A7UQT9|A0A2A7UQT9_COMTR LysR family transcriptional regulator OS=Comamonas terrigena OX=32013 GN=CRM82_02540 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.4 TKK25 pKa = 10.4 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4009
0
4009
1308246
31
3971
326.3
35.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.115 ± 0.056
0.922 ± 0.013
4.975 ± 0.03
4.873 ± 0.039
3.233 ± 0.027
8.293 ± 0.047
2.392 ± 0.019
4.051 ± 0.03
3.173 ± 0.035
10.991 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.023
2.586 ± 0.032
5.226 ± 0.035
5.071 ± 0.035
6.245 ± 0.041
5.463 ± 0.039
5.341 ± 0.045
7.705 ± 0.038
1.605 ± 0.02
2.18 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here