Amino acid dipepetide frequency for bacterium HR37

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.889AlaAla: 3.889 ± 0.096	0.651AlaCys: 0.651 ± 0.031	2.6AlaAsp: 2.6 ± 0.066	4.215AlaGlu: 4.215 ± 0.08	3.269AlaPhe: 3.269 ± 0.084	4.691AlaGly: 4.691 ± 0.095	1.122AlaHis: 1.122 ± 0.038	5.476AlaIle: 5.476 ± 0.092	4.151AlaLys: 4.151 ± 0.075	7.739AlaLeu: 7.739 ± 0.107	1.441AlaMet: 1.441 ± 0.039	1.916AlaAsn: 1.916 ± 0.063	1.897AlaPro: 1.897 ± 0.058	1.714AlaGln: 1.714 ± 0.055	4.038AlaArg: 4.038 ± 0.074	3.989AlaSer: 3.989 ± 0.068	2.832AlaThr: 2.832 ± 0.067	4.983AlaVal: 4.983 ± 0.083	0.594AlaTrp: 0.594 ± 0.031	2.177AlaTyr: 2.177 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.587CysAla: 0.587 ± 0.031	0.138CysCys: 0.138 ± 0.015	0.433CysAsp: 0.433 ± 0.026	0.57CysGlu: 0.57 ± 0.029	0.466CysPhe: 0.466 ± 0.025	0.972CysGly: 0.972 ± 0.047	0.215CysHis: 0.215 ± 0.026	0.695CysIle: 0.695 ± 0.034	0.508CysLys: 0.508 ± 0.026	0.826CysLeu: 0.826 ± 0.032	0.18CysMet: 0.18 ± 0.016	0.366CysAsn: 0.366 ± 0.023	0.593CysPro: 0.593 ± 0.033	0.194CysGln: 0.194 ± 0.016	0.546CysArg: 0.546 ± 0.028	0.652CysSer: 0.652 ± 0.03	0.385CysThr: 0.385 ± 0.025	0.758CysVal: 0.758 ± 0.031	0.112CysTrp: 0.112 ± 0.012	0.367CysTyr: 0.367 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	2.69AspAla: 2.69 ± 0.06	0.458AspCys: 0.458 ± 0.028	1.828AspAsp: 1.828 ± 0.064	3.696AspGlu: 3.696 ± 0.086	2.731AspPhe: 2.731 ± 0.066	3.205AspGly: 3.205 ± 0.075	0.627AspHis: 0.627 ± 0.028	4.988AspIle: 4.988 ± 0.089	2.932AspLys: 2.932 ± 0.065	4.628AspLeu: 4.628 ± 0.078	1.09AspMet: 1.09 ± 0.033	1.483AspAsn: 1.483 ± 0.056	2.466AspPro: 2.466 ± 0.074	0.774AspGln: 0.774 ± 0.036	2.758AspArg: 2.758 ± 0.06	2.674AspSer: 2.674 ± 0.064	2.442AspThr: 2.442 ± 0.058	3.608AspVal: 3.608 ± 0.075	0.478AspTrp: 0.478 ± 0.027	1.774AspTyr: 1.774 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	5.537GluAla: 5.537 ± 0.094	0.589GluCys: 0.589 ± 0.03	3.901GluAsp: 3.901 ± 0.069	8.169GluGlu: 8.169 ± 0.151	3.387GluPhe: 3.387 ± 0.065	5.433GluGly: 5.433 ± 0.096	1.106GluHis: 1.106 ± 0.04	6.88GluIle: 6.88 ± 0.112	6.412GluLys: 6.412 ± 0.122	8.138GluLeu: 8.138 ± 0.127	1.533GluMet: 1.533 ± 0.042	2.987GluAsn: 2.987 ± 0.067	2.334GluPro: 2.334 ± 0.063	1.708GluGln: 1.708 ± 0.051	5.515GluArg: 5.515 ± 0.093	3.986GluSer: 3.986 ± 0.079	3.271GluThr: 3.271 ± 0.076	6.375GluVal: 6.375 ± 0.105	0.651GluTrp: 0.651 ± 0.028	2.22GluTyr: 2.22 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.841PheAla: 2.841 ± 0.074	0.56PheCys: 0.56 ± 0.031	2.354PheAsp: 2.354 ± 0.059	3.167PheGlu: 3.167 ± 0.069	2.849PhePhe: 2.849 ± 0.083	3.592PheGly: 3.592 ± 0.088	0.739PheHis: 0.739 ± 0.037	3.749PheIle: 3.749 ± 0.083	3.072PheLys: 3.072 ± 0.074	5.426PheLeu: 5.426 ± 0.111	0.947PheMet: 0.947 ± 0.041	1.808PheAsn: 1.808 ± 0.055	2.144PhePro: 2.144 ± 0.059	0.961PheGln: 0.961 ± 0.035	2.421PheArg: 2.421 ± 0.06	3.627PheSer: 3.627 ± 0.078	2.266PheThr: 2.266 ± 0.058	3.566PheVal: 3.566 ± 0.079	0.586PheTrp: 0.586 ± 0.034	1.697PheTyr: 1.697 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.568GlyAla: 4.568 ± 0.088	0.902GlyCys: 0.902 ± 0.041	3.539GlyAsp: 3.539 ± 0.074	5.308GlyGlu: 5.308 ± 0.092	4.373GlyPhe: 4.373 ± 0.087	5.404GlyGly: 5.404 ± 0.108	1.178GlyHis: 1.178 ± 0.047	7.215GlyIle: 7.215 ± 0.11	5.831GlyLys: 5.831 ± 0.096	6.636GlyLeu: 6.636 ± 0.105	1.773GlyMet: 1.773 ± 0.052	2.487GlyAsn: 2.487 ± 0.066	1.826GlyPro: 1.826 ± 0.052	1.391GlyGln: 1.391 ± 0.047	4.366GlyArg: 4.366 ± 0.087	4.197GlySer: 4.197 ± 0.092	3.504GlyThr: 3.504 ± 0.072	5.974GlyVal: 5.974 ± 0.107	0.952GlyTrp: 0.952 ± 0.038	2.79GlyTyr: 2.79 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	0.932HisAla: 0.932 ± 0.031	0.17HisCys: 0.17 ± 0.015	0.631HisAsp: 0.631 ± 0.032	0.914HisGlu: 0.914 ± 0.033	0.855HisPhe: 0.855 ± 0.035	1.184HisGly: 1.184 ± 0.045	0.409HisHis: 0.409 ± 0.026	1.469HisIle: 1.469 ± 0.038	0.821HisLys: 0.821 ± 0.037	1.745HisLeu: 1.745 ± 0.049	0.39HisMet: 0.39 ± 0.02	0.559HisAsn: 0.559 ± 0.029	1.08HisPro: 1.08 ± 0.041	0.385HisGln: 0.385 ± 0.023	1.024HisArg: 1.024 ± 0.041	0.953HisSer: 0.953 ± 0.032	0.86HisThr: 0.86 ± 0.027	1.02HisVal: 1.02 ± 0.039	0.184HisTrp: 0.184 ± 0.017	0.55HisTyr: 0.55 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.931IleAla: 5.931 ± 0.084	0.806IleCys: 0.806 ± 0.037	3.768IleAsp: 3.768 ± 0.071	6.887IleGlu: 6.887 ± 0.116	2.855IlePhe: 2.855 ± 0.065	5.907IleGly: 5.907 ± 0.096	1.272IleHis: 1.272 ± 0.04	4.772IleIle: 4.772 ± 0.081	5.869IleLys: 5.869 ± 0.095	7.774IleLeu: 7.774 ± 0.085	1.418IleMet: 1.418 ± 0.045	2.843IleAsn: 2.843 ± 0.067	4.209IlePro: 4.209 ± 0.076	1.824IleGln: 1.824 ± 0.042	5.057IleArg: 5.057 ± 0.086	5.3IleSer: 5.3 ± 0.08	4.139IleThr: 4.139 ± 0.08	6.046IleVal: 6.046 ± 0.093	0.714IleTrp: 0.714 ± 0.036	2.42IleTyr: 2.42 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	4.717LysAla: 4.717 ± 0.079	0.392LysCys: 0.392 ± 0.028	3.642LysAsp: 3.642 ± 0.076	7.719LysGlu: 7.719 ± 0.123	1.751LysPhe: 1.751 ± 0.047	5.073LysGly: 5.073 ± 0.084	1.188LysHis: 1.188 ± 0.042	4.718LysIle: 4.718 ± 0.094	5.631LysLys: 5.631 ± 0.11	6.285LysLeu: 6.285 ± 0.104	1.223LysMet: 1.223 ± 0.045	2.866LysAsn: 2.866 ± 0.07	2.751LysPro: 2.751 ± 0.062	1.684LysGln: 1.684 ± 0.046	5.068LysArg: 5.068 ± 0.088	3.507LysSer: 3.507 ± 0.072	3.519LysThr: 3.519 ± 0.075	4.891LysVal: 4.891 ± 0.095	0.577LysTrp: 0.577 ± 0.028	1.795LysTyr: 1.795 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	6.49LeuAla: 6.49 ± 0.095	1.013LeuCys: 1.013 ± 0.042	4.737LeuAsp: 4.737 ± 0.094	7.762LeuGlu: 7.762 ± 0.126	4.914LeuPhe: 4.914 ± 0.108	7.977LeuGly: 7.977 ± 0.11	1.475LeuHis: 1.475 ± 0.044	7.727LeuIle: 7.727 ± 0.124	8.058LeuLys: 8.058 ± 0.121	9.827LeuLeu: 9.827 ± 0.163	1.796LeuMet: 1.796 ± 0.051	3.899LeuAsn: 3.899 ± 0.073	4.15LeuPro: 4.15 ± 0.068	2.08LeuGln: 2.08 ± 0.052	6.305LeuArg: 6.305 ± 0.103	7.759LeuSer: 7.759 ± 0.125	4.563LeuThr: 4.563 ± 0.083	7.055LeuVal: 7.055 ± 0.103	1.013LeuTrp: 1.013 ± 0.041	3.217LeuTyr: 3.217 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	1.542MetAla: 1.542 ± 0.045	0.189MetCys: 0.189 ± 0.016	1.099MetAsp: 1.099 ± 0.041	1.652MetGlu: 1.652 ± 0.058	0.751MetPhe: 0.751 ± 0.032	1.847MetGly: 1.847 ± 0.05	0.297MetHis: 0.297 ± 0.021	1.326MetIle: 1.326 ± 0.045	1.526MetLys: 1.526 ± 0.041	1.922MetLeu: 1.922 ± 0.049	0.388MetMet: 0.388 ± 0.024	0.689MetAsn: 0.689 ± 0.031	0.874MetPro: 0.874 ± 0.034	0.512MetGln: 0.512 ± 0.029	1.256MetArg: 1.256 ± 0.038	1.21MetSer: 1.21 ± 0.042	0.74MetThr: 0.74 ± 0.031	1.673MetVal: 1.673 ± 0.05	0.186MetTrp: 0.186 ± 0.015	0.473MetTyr: 0.473 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.04AsnAla: 2.04 ± 0.05	0.386AsnCys: 0.386 ± 0.024	1.144AsnAsp: 1.144 ± 0.042	2.14AsnGlu: 2.14 ± 0.055	1.494AsnPhe: 1.494 ± 0.046	2.305AsnGly: 2.305 ± 0.055	0.679AsnHis: 0.679 ± 0.035	2.905AsnIle: 2.905 ± 0.061	1.885AsnLys: 1.885 ± 0.055	4.392AsnLeu: 4.392 ± 0.088	0.794AsnMet: 0.794 ± 0.038	1.176AsnAsn: 1.176 ± 0.044	2.699AsnPro: 2.699 ± 0.057	1.003AsnGln: 1.003 ± 0.043	2.385AsnArg: 2.385 ± 0.06	2.038AsnSer: 2.038 ± 0.054	1.855AsnThr: 1.855 ± 0.049	2.347AsnVal: 2.347 ± 0.064	0.442AsnTrp: 0.442 ± 0.022	1.26AsnTyr: 1.26 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	2.096ProAla: 2.096 ± 0.061	0.324ProCys: 0.324 ± 0.023	2.558ProAsp: 2.558 ± 0.064	3.88ProGlu: 3.88 ± 0.08	2.361ProPhe: 2.361 ± 0.06	2.991ProGly: 2.991 ± 0.073	0.853ProHis: 0.853 ± 0.04	2.752ProIle: 2.752 ± 0.064	2.42ProLys: 2.42 ± 0.064	3.962ProLeu: 3.962 ± 0.079	0.733ProMet: 0.733 ± 0.035	1.33ProAsn: 1.33 ± 0.05	1.903ProPro: 1.903 ± 0.055	1.282ProGln: 1.282 ± 0.041	2.17ProArg: 2.17 ± 0.058	2.679ProSer: 2.679 ± 0.06	1.757ProThr: 1.757 ± 0.049	3.542ProVal: 3.542 ± 0.071	0.458ProTrp: 0.458 ± 0.025	1.587ProTyr: 1.587 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	1.684GlnAla: 1.684 ± 0.055	0.143GlnCys: 0.143 ± 0.013	0.947GlnAsp: 0.947 ± 0.036	1.801GlnGlu: 1.801 ± 0.058	0.86GlnPhe: 0.86 ± 0.04	1.675GlnGly: 1.675 ± 0.047	0.315GlnHis: 0.315 ± 0.02	2.045GlnIle: 2.045 ± 0.055	1.73GlnLys: 1.73 ± 0.061	2.031GlnLeu: 2.031 ± 0.06	0.564GlnMet: 0.564 ± 0.026	0.893GlnAsn: 0.893 ± 0.036	0.745GlnPro: 0.745 ± 0.029	0.542GlnGln: 0.542 ± 0.031	1.764GlnArg: 1.764 ± 0.049	1.188GlnSer: 1.188 ± 0.041	1.151GlnThr: 1.151 ± 0.041	1.729GlnVal: 1.729 ± 0.055	0.208GlnTrp: 0.208 ± 0.02	0.551GlnTyr: 0.551 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	4.027ArgAla: 4.027 ± 0.075	0.574ArgCys: 0.574 ± 0.029	3.063ArgAsp: 3.063 ± 0.069	6.081ArgGlu: 6.081 ± 0.1	3.408ArgPhe: 3.408 ± 0.071	4.243ArgGly: 4.243 ± 0.078	0.825ArgHis: 0.825 ± 0.036	5.35ArgIle: 5.35 ± 0.092	4.254ArgLys: 4.254 ± 0.076	5.943ArgLeu: 5.943 ± 0.105	1.318ArgMet: 1.318 ± 0.044	2.282ArgAsn: 2.282 ± 0.054	1.864ArgPro: 1.864 ± 0.06	1.283ArgGln: 1.283 ± 0.045	3.846ArgArg: 3.846 ± 0.077	2.871ArgSer: 2.871 ± 0.061	2.274ArgThr: 2.274 ± 0.058	5.346ArgVal: 5.346 ± 0.087	0.677ArgTrp: 0.677 ± 0.032	2.392ArgTyr: 2.392 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	3.429SerAla: 3.429 ± 0.072	0.609SerCys: 0.609 ± 0.034	2.789SerAsp: 2.789 ± 0.057	4.032SerGlu: 4.032 ± 0.077	3.429SerPhe: 3.429 ± 0.076	5.046SerGly: 5.046 ± 0.086	1.02SerHis: 1.02 ± 0.036	4.922SerIle: 4.922 ± 0.091	3.838SerLys: 3.838 ± 0.082	6.887SerLeu: 6.887 ± 0.11	1.311SerMet: 1.311 ± 0.036	1.984SerAsn: 1.984 ± 0.053	2.762SerPro: 2.762 ± 0.061	1.507SerGln: 1.507 ± 0.049	3.589SerArg: 3.589 ± 0.077	4.242SerSer: 4.242 ± 0.081	2.818SerThr: 2.818 ± 0.063	4.598SerVal: 4.598 ± 0.087	0.683SerTrp: 0.683 ± 0.034	2.204SerTyr: 2.204 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	2.932ThrAla: 2.932 ± 0.065	0.384ThrCys: 0.384 ± 0.023	2.107ThrAsp: 2.107 ± 0.053	3.095ThrGlu: 3.095 ± 0.067	2.035ThrPhe: 2.035 ± 0.055	4.011ThrGly: 4.011 ± 0.074	0.943ThrHis: 0.943 ± 0.042	3.253ThrIle: 3.253 ± 0.076	2.543ThrLys: 2.543 ± 0.066	5.238ThrLeu: 5.238 ± 0.09	0.82ThrMet: 0.82 ± 0.034	1.472ThrAsn: 1.472 ± 0.046	2.532ThrPro: 2.532 ± 0.055	1.232ThrGln: 1.232 ± 0.044	2.509ThrArg: 2.509 ± 0.054	2.718ThrSer: 2.718 ± 0.061	2.393ThrThr: 2.393 ± 0.063	4.004ThrVal: 4.004 ± 0.082	0.406ThrTrp: 0.406 ± 0.023	1.552ThrTyr: 1.552 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.995ValAla: 4.995 ± 0.091	0.839ValCys: 0.839 ± 0.034	4.157ValAsp: 4.157 ± 0.092	5.957ValGlu: 5.957 ± 0.099	4.554ValPhe: 4.554 ± 0.096	5.234ValGly: 5.234 ± 0.115	1.032ValHis: 1.032 ± 0.039	6.109ValIle: 6.109 ± 0.092	4.917ValLys: 4.917 ± 0.087	7.94ValLeu: 7.94 ± 0.103	1.504ValMet: 1.504 ± 0.043	2.701ValAsn: 2.701 ± 0.064	2.849ValPro: 2.849 ± 0.053	1.354ValGln: 1.354 ± 0.045	4.279ValArg: 4.279 ± 0.076	5.462ValSer: 5.462 ± 0.093	3.426ValThr: 3.426 ± 0.07	7.09ValVal: 7.09 ± 0.133	0.698ValTrp: 0.698 ± 0.034	2.47ValTyr: 2.47 ± 0.061	0.0ValXaa: 0.0 ± 0.0
Trp	0.605TrpAla: 0.605 ± 0.029	0.097TrpCys: 0.097 ± 0.011	0.517TrpAsp: 0.517 ± 0.03	0.748TrpGlu: 0.748 ± 0.035	0.548TrpPhe: 0.548 ± 0.033	0.762TrpGly: 0.762 ± 0.03	0.209TrpHis: 0.209 ± 0.016	0.871TrpIle: 0.871 ± 0.036	0.709TrpLys: 0.709 ± 0.031	0.983TrpLeu: 0.983 ± 0.037	0.244TrpMet: 0.244 ± 0.018	0.417TrpAsn: 0.417 ± 0.025	0.278TrpPro: 0.278 ± 0.024	0.346TrpGln: 0.346 ± 0.021	0.563TrpArg: 0.563 ± 0.03	0.529TrpSer: 0.529 ± 0.025	0.378TrpThr: 0.378 ± 0.026	0.789TrpVal: 0.789 ± 0.039	0.166TrpTrp: 0.166 ± 0.019	0.362TrpTyr: 0.362 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.851TyrAla: 1.851 ± 0.05	0.34TyrCys: 0.34 ± 0.022	1.515TyrAsp: 1.515 ± 0.046	2.339TyrGlu: 2.339 ± 0.064	1.485TyrPhe: 1.485 ± 0.052	2.663TyrGly: 2.663 ± 0.064	0.632TyrHis: 0.632 ± 0.027	2.587TyrIle: 2.587 ± 0.063	1.892TyrLys: 1.892 ± 0.047	3.494TyrLeu: 3.494 ± 0.079	0.682TyrMet: 0.682 ± 0.032	1.213TyrAsn: 1.213 ± 0.042	1.681TyrPro: 1.681 ± 0.052	0.789TyrGln: 0.789 ± 0.032	2.462TyrArg: 2.462 ± 0.068	2.115TyrSer: 2.115 ± 0.061	1.604TyrThr: 1.604 ± 0.048	2.174TyrVal: 2.174 ± 0.059	0.338TyrTrp: 0.338 ± 0.02	1.18TyrTyr: 1.18 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2382 proteins (740518 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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