Bambusicola thoracicus (Chinese bamboo-partridge) (Perdix thoracica)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17669 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P4SU70|A0A2P4SU70_BAMTH Uncharacterized protein OS=Bambusicola thoracicus OX=9083 GN=CIB84_008584 PE=4 SV=1
NN1 pKa = 7.6 CPLLPNSGQEE11 pKa = 5.08 DD12 pKa = 3.93 FDD14 pKa = 5.41 KK15 pKa = 11.13 DD16 pKa = 3.96 GKK18 pKa = 11.32 GDD20 pKa = 3.67 ACDD23 pKa = 4.44 EE24 pKa = 4.89 DD25 pKa = 5.03 DD26 pKa = 6.11 DD27 pKa = 5.28 NDD29 pKa = 4.37 GVEE32 pKa = 4.65 DD33 pKa = 5.47 DD34 pKa = 5.49 KK35 pKa = 12.08 DD36 pKa = 3.71 NCPLLFNPRR45 pKa = 11.84 QFDD48 pKa = 3.57 YY49 pKa = 11.45 DD50 pKa = 3.52 KK51 pKa = 11.69 DD52 pKa = 4.14 EE53 pKa = 4.7 VGDD56 pKa = 3.79 RR57 pKa = 11.84 CDD59 pKa = 3.2 NCPYY63 pKa = 10.11 VHH65 pKa = 6.82 NPAQIDD71 pKa = 3.5 TDD73 pKa = 3.98 NNGEE77 pKa = 3.99 GDD79 pKa = 3.81 SCAVDD84 pKa = 3.25 IDD86 pKa = 4.11 GDD88 pKa = 4.29 DD89 pKa = 3.67 IFNEE93 pKa = 4.17 RR94 pKa = 11.84 DD95 pKa = 3.22 NCPYY99 pKa = 10.71 VYY101 pKa = 9.85 NTDD104 pKa = 3.57 QSDD107 pKa = 3.51 TDD109 pKa = 3.82 GDD111 pKa = 4.48 GVGDD115 pKa = 3.71 QCDD118 pKa = 4.06 NCPLMHH124 pKa = 7.14 NPDD127 pKa = 3.26 QTDD130 pKa = 3.12 ADD132 pKa = 3.89 NDD134 pKa = 4.23 LVGDD138 pKa = 3.7 QCDD141 pKa = 3.64 NNEE144 pKa = 5.1 DD145 pKa = 3.49 IDD147 pKa = 4.85 EE148 pKa = 5.38 DD149 pKa = 3.95 GHH151 pKa = 6.99 QNNQDD156 pKa = 3.29 NCPYY160 pKa = 10.32 IPNANQADD168 pKa = 3.79 HH169 pKa = 7.42 DD170 pKa = 4.64 KK171 pKa = 11.17 DD172 pKa = 4.07 GKK174 pKa = 11.27 GDD176 pKa = 3.62 ACDD179 pKa = 4.6 PDD181 pKa = 5.2 DD182 pKa = 6.11 DD183 pKa = 5.43 NDD185 pKa = 5.38 GIPDD189 pKa = 3.84 DD190 pKa = 4.96 RR191 pKa = 11.84 DD192 pKa = 3.43 NCRR195 pKa = 11.84 LRR197 pKa = 11.84 YY198 pKa = 9.88 NPEE201 pKa = 3.86 QEE203 pKa = 5.42 DD204 pKa = 3.5 SDD206 pKa = 4.25 GDD208 pKa = 3.89 GRR210 pKa = 11.84 GDD212 pKa = 3.12 ICKK215 pKa = 10.38 DD216 pKa = 3.51 DD217 pKa = 4.78 FDD219 pKa = 6.91 DD220 pKa = 6.6 DD221 pKa = 4.41 SVPDD225 pKa = 3.82 IFDD228 pKa = 3.35 VCPEE232 pKa = 3.87 NNAISEE238 pKa = 4.09 TDD240 pKa = 3.55 FRR242 pKa = 11.84 KK243 pKa = 9.59 FQMVPLDD250 pKa = 3.85 PKK252 pKa = 10.2 GTAQIDD258 pKa = 3.99 PNWVIRR264 pKa = 11.84 HH265 pKa = 4.99 QGKK268 pKa = 9.11 EE269 pKa = 3.87 LVQTANSDD277 pKa = 3.25 PGIAVGYY284 pKa = 10.41 DD285 pKa = 3.19 EE286 pKa = 5.41 FSSVDD291 pKa = 3.71 FSGTFYY297 pKa = 11.53 VNTDD301 pKa = 3.3 RR302 pKa = 11.84 DD303 pKa = 3.36 DD304 pKa = 4.91 DD305 pKa = 4.04 YY306 pKa = 12.01 AGFVFGYY313 pKa = 9.43 QSSSRR318 pKa = 11.84 FYY320 pKa = 10.37 VLMWKK325 pKa = 9.65 QVTQTYY331 pKa = 8.8 WEE333 pKa = 4.7 DD334 pKa = 3.53 KK335 pKa = 7.69 PTRR338 pKa = 11.84 AYY340 pKa = 10.31 GYY342 pKa = 9.8 SGVSLKK348 pKa = 10.51 VVNSTTGTGEE358 pKa = 4.03 HH359 pKa = 6.41 LRR361 pKa = 11.84 NALWHH366 pKa = 6.19 TGNTPGQVRR375 pKa = 11.84 DD376 pKa = 4.29 LKK378 pKa = 10.93 LHH380 pKa = 5.95 HH381 pKa = 7.02 CGG383 pKa = 3.92
Molecular weight: 42.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.665
IPC2_protein 3.706
IPC_protein 3.783
Toseland 3.528
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.592
Grimsley 3.427
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.228
Thurlkill 3.592
EMBOSS 3.77
Sillero 3.91
Patrickios 1.507
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A2P4SFI2|A0A2P4SFI2_BAMTH Uncharacterized protein OS=Bambusicola thoracicus OX=9083 GN=CIB84_013417 PE=3 SV=1
GG1 pKa = 7.03 ARR3 pKa = 11.84 RR4 pKa = 11.84 SPRR7 pKa = 11.84 AAAPAARR14 pKa = 11.84 RR15 pKa = 11.84 SAAPRR20 pKa = 11.84 RR21 pKa = 11.84 AAQPGSAAQRR31 pKa = 11.84 PRR33 pKa = 11.84 EE34 pKa = 3.96 RR35 pKa = 11.84 PRR37 pKa = 11.84 GGRR40 pKa = 11.84 GRR42 pKa = 11.84 RR43 pKa = 11.84 ARR45 pKa = 11.84 VPPRR49 pKa = 11.84 QLRR52 pKa = 11.84 RR53 pKa = 11.84 GLRR56 pKa = 11.84 GVLLVRR62 pKa = 11.84 RR63 pKa = 11.84 APHH66 pKa = 5.59 QGGVPPLRR74 pKa = 11.84 HH75 pKa = 6.12 PGAARR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 3.88
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.896
Toseland 13.056
ProMoST 13.568
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.559
Grimsley 13.1
Solomon 13.554
Lehninger 13.466
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.281
IPC_peptide 13.568
IPC2_peptide 12.559
IPC2.peptide.svr19 9.255
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17669
0
17669
6035687
8
7645
341.6
38.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.096 ± 0.024
2.319 ± 0.015
4.867 ± 0.013
7.171 ± 0.029
3.766 ± 0.016
6.23 ± 0.025
2.519 ± 0.01
4.579 ± 0.016
5.928 ± 0.025
9.772 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.25 ± 0.008
3.819 ± 0.018
5.658 ± 0.025
4.605 ± 0.019
5.457 ± 0.018
8.322 ± 0.027
5.281 ± 0.016
6.32 ± 0.018
1.187 ± 0.008
2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here