White spot syndrome virus (WSSV) (White spot bacilliform virus)
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 532 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q77EH0|Q77EH0_WSSV WSSV489 OS=White spot syndrome virus OX=92652 GN=431 PE=4 SV=1
MM1 pKa = 6.9 VFSWLMSDD9 pKa = 3.8 RR10 pKa = 11.84 SIFKK14 pKa = 7.94 TTNFTSTLNAGTEE27 pKa = 4.18 FSTAAAAASEE37 pKa = 4.2 VEE39 pKa = 4.35 EE40 pKa = 4.25 EE41 pKa = 4.75 DD42 pKa = 4.34 EE43 pKa = 4.07 EE44 pKa = 4.6 LLLFFLVSLMIEE56 pKa = 3.97 VSTKK60 pKa = 10.36 FEE62 pKa = 3.86 TAFFKK67 pKa = 10.51 TCFLVSVIAPIILSVLEE84 pKa = 4.73 EE85 pKa = 3.93 IASFSCLALEE95 pKa = 4.04 LAMRR99 pKa = 11.84 RR100 pKa = 11.84 SHH102 pKa = 7.23 ISLGCPCSFVINSSIFFLLSFPFEE126 pKa = 4.03 ILVTICMANTIDD138 pKa = 3.62 FSEE141 pKa = 4.42 YY142 pKa = 10.5 LSEE145 pKa = 4.61 LLCLLLMLLFSVV157 pKa = 4.54
Molecular weight: 17.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.97
IPC2_protein 4.279
IPC_protein 4.126
Toseland 3.986
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.884
Rodwell 3.961
Grimsley 3.91
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.215
Thurlkill 3.986
EMBOSS 3.897
Sillero 4.228
Patrickios 1.914
IPC_peptide 4.05
IPC2_peptide 4.215
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|Q77F27|Q77F27_WSSV WSSV255 OS=White spot syndrome virus OX=92652 GN=200 PE=4 SV=1
MM1 pKa = 7.33 TKK3 pKa = 10.24 YY4 pKa = 10.4 PEE6 pKa = 4.08 NKK8 pKa = 9.74 RR9 pKa = 11.84 LLSRR13 pKa = 11.84 NKK15 pKa = 8.59 EE16 pKa = 4.28 TLKK19 pKa = 10.33 MVARR23 pKa = 11.84 SSKK26 pKa = 7.8 TKK28 pKa = 10.2 SRR30 pKa = 11.84 RR31 pKa = 11.84 GSKK34 pKa = 9.86 KK35 pKa = 9.73 RR36 pKa = 11.84 STTAGRR42 pKa = 11.84 ISKK45 pKa = 9.98 RR46 pKa = 11.84 RR47 pKa = 11.84 SPSMKK52 pKa = 9.48 KK53 pKa = 9.83 RR54 pKa = 11.84 AGKK57 pKa = 10.17 KK58 pKa = 8.75 SSTVRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 SSKK68 pKa = 10.18 SGKK71 pKa = 9.73 KK72 pKa = 9.68 SGARR76 pKa = 11.84 KK77 pKa = 9.36 SRR79 pKa = 11.84 RR80 pKa = 3.37
Molecular weight: 9.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.362
IPC2_protein 10.891
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.354
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.012
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
531
1
532
125083
60
6077
235.1
26.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.787 ± 0.171
1.919 ± 0.082
5.091 ± 0.112
6.565 ± 0.179
4.862 ± 0.117
5.334 ± 0.287
1.983 ± 0.073
5.956 ± 0.103
6.737 ± 0.139
9.037 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.815 ± 0.067
5.397 ± 0.145
4.657 ± 0.188
2.912 ± 0.121
5.205 ± 0.107
9.904 ± 0.231
5.992 ± 0.105
6.226 ± 0.081
0.937 ± 0.053
2.682 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here