White spot syndrome virus (WSSV) (White spot bacilliform virus)

Taxonomy: Viruses; Naldaviricetes; Nimaviridae; Whispovirus; White spot syndrome virus

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q77EH0|Q77EH0_WSSV WSSV489 OS=White spot syndrome virus OX=92652 GN=431 PE=4 SV=1
MM1 pKa = 6.9VFSWLMSDD9 pKa = 3.8RR10 pKa = 11.84SIFKK14 pKa = 7.94TTNFTSTLNAGTEE27 pKa = 4.18FSTAAAAASEE37 pKa = 4.2VEE39 pKa = 4.35EE40 pKa = 4.25EE41 pKa = 4.75DD42 pKa = 4.34EE43 pKa = 4.07EE44 pKa = 4.6LLLFFLVSLMIEE56 pKa = 3.97VSTKK60 pKa = 10.36FEE62 pKa = 3.86TAFFKK67 pKa = 10.51TCFLVSVIAPIILSVLEE84 pKa = 4.73EE85 pKa = 3.93IASFSCLALEE95 pKa = 4.04LAMRR99 pKa = 11.84RR100 pKa = 11.84SHH102 pKa = 7.23ISLGCPCSFVINSSIFFLLSFPFEE126 pKa = 4.03ILVTICMANTIDD138 pKa = 3.62FSEE141 pKa = 4.42YY142 pKa = 10.5LSEE145 pKa = 4.61LLCLLLMLLFSVV157 pKa = 4.54

Molecular weight:
17.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q77F27|Q77F27_WSSV WSSV255 OS=White spot syndrome virus OX=92652 GN=200 PE=4 SV=1
MM1 pKa = 7.33TKK3 pKa = 10.24YY4 pKa = 10.4PEE6 pKa = 4.08NKK8 pKa = 9.74RR9 pKa = 11.84LLSRR13 pKa = 11.84NKK15 pKa = 8.59EE16 pKa = 4.28TLKK19 pKa = 10.33MVARR23 pKa = 11.84SSKK26 pKa = 7.8TKK28 pKa = 10.2SRR30 pKa = 11.84RR31 pKa = 11.84GSKK34 pKa = 9.86KK35 pKa = 9.73RR36 pKa = 11.84STTAGRR42 pKa = 11.84ISKK45 pKa = 9.98RR46 pKa = 11.84RR47 pKa = 11.84SPSMKK52 pKa = 9.48KK53 pKa = 9.83RR54 pKa = 11.84AGKK57 pKa = 10.17KK58 pKa = 8.75SSTVRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84SSKK68 pKa = 10.18SGKK71 pKa = 9.73KK72 pKa = 9.68SGARR76 pKa = 11.84KK77 pKa = 9.36SRR79 pKa = 11.84RR80 pKa = 3.37

Molecular weight:
9.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

531

1

532

125083

60

6077

235.1

26.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.787 ± 0.171

1.919 ± 0.082

5.091 ± 0.112

6.565 ± 0.179

4.862 ± 0.117

5.334 ± 0.287

1.983 ± 0.073

5.956 ± 0.103

6.737 ± 0.139

9.037 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.067

5.397 ± 0.145

4.657 ± 0.188

2.912 ± 0.121

5.205 ± 0.107

9.904 ± 0.231

5.992 ± 0.105

6.226 ± 0.081

0.937 ± 0.053

2.682 ± 0.063

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski