Cleome droserifolia amalgavirus 1
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIN0|A0A2Z6JIN0_9VIRU ORF1p OS=Cleome droserifolia amalgavirus 1 OX=2069321 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.92 AEE3 pKa = 4.17 HH4 pKa = 6.88 QDD6 pKa = 3.52 PQAQGGAVGGDD17 pKa = 3.38 VEE19 pKa = 4.85 EE20 pKa = 4.5 VNLLEE25 pKa = 5.01 GIGGIPTAAEE35 pKa = 3.7 EE36 pKa = 4.07 RR37 pKa = 11.84 NRR39 pKa = 11.84 LQEE42 pKa = 3.95 ALRR45 pKa = 11.84 PLIANGVNIEE55 pKa = 4.39 RR56 pKa = 11.84 ITVNDD61 pKa = 3.94 CLRR64 pKa = 11.84 LGFTVEE70 pKa = 3.68 QLARR74 pKa = 11.84 AIRR77 pKa = 11.84 VLTSIASDD85 pKa = 3.43 EE86 pKa = 4.04 MRR88 pKa = 11.84 DD89 pKa = 3.83 LIFSQAHH96 pKa = 6.53 LSRR99 pKa = 11.84 LAPAARR105 pKa = 11.84 SVTLDD110 pKa = 3.79 HH111 pKa = 7.11 ILAICEE117 pKa = 3.97 WMKK120 pKa = 10.15 TEE122 pKa = 5.42 RR123 pKa = 11.84 GAQAIRR129 pKa = 11.84 NLQTSAKK136 pKa = 7.74 QEE138 pKa = 3.61 RR139 pKa = 11.84 RR140 pKa = 11.84 AVGGRR145 pKa = 11.84 TVADD149 pKa = 3.35 VSMLQAFNQQVADD162 pKa = 3.88 WSAQVKK168 pKa = 7.77 EE169 pKa = 3.91 ARR171 pKa = 11.84 KK172 pKa = 9.78 EE173 pKa = 3.89 IEE175 pKa = 3.87 EE176 pKa = 4.03 EE177 pKa = 4.03 MAEE180 pKa = 4.73 LRR182 pKa = 11.84 AQLALKK188 pKa = 9.51 QQEE191 pKa = 4.71 LNDD194 pKa = 3.53 SLAAINEE201 pKa = 4.68 SYY203 pKa = 9.91 WPASSYY209 pKa = 10.33 KK210 pKa = 10.5 APALNVVQSQAYY222 pKa = 8.87 ALYY225 pKa = 10.54 AADD228 pKa = 4.26 CRR230 pKa = 11.84 RR231 pKa = 11.84 SGTVPLPAGSLGYY244 pKa = 10.37 SKK246 pKa = 10.83 AVEE249 pKa = 4.04 LFGQQVRR256 pKa = 11.84 EE257 pKa = 4.05 DD258 pKa = 3.58 HH259 pKa = 5.63 QLQYY263 pKa = 11.34 LRR265 pKa = 11.84 AEE267 pKa = 4.45 EE268 pKa = 4.64 ARR270 pKa = 11.84 DD271 pKa = 3.56 TLSEE275 pKa = 4.03 YY276 pKa = 10.66 LQRR279 pKa = 11.84 LVGLKK284 pKa = 10.1 AGQGRR289 pKa = 11.84 RR290 pKa = 11.84 RR291 pKa = 11.84 EE292 pKa = 4.23 VEE294 pKa = 3.66 NFRR297 pKa = 11.84 AILAGMGVAAADD309 pKa = 3.74 AVPARR314 pKa = 11.84 AEE316 pKa = 3.94 EE317 pKa = 4.12 VAIEE321 pKa = 3.99 QVGGGEE327 pKa = 4.11 DD328 pKa = 3.31 QPAGEE333 pKa = 4.68 RR334 pKa = 11.84 APDD337 pKa = 3.48 QAPEE341 pKa = 4.36 GGDD344 pKa = 3.03 AGGADD349 pKa = 3.84 EE350 pKa = 5.81 GEE352 pKa = 4.28 AAGGHH357 pKa = 4.43 QTPPRR362 pKa = 11.84 PAAEE366 pKa = 4.02 DD367 pKa = 3.3 RR368 pKa = 11.84 RR369 pKa = 11.84 GGRR372 pKa = 11.84 RR373 pKa = 11.84 VTRR376 pKa = 11.84 RR377 pKa = 11.84 RR378 pKa = 11.84 DD379 pKa = 3.39 DD380 pKa = 3.59 GAGPSTRR387 pKa = 11.84 RR388 pKa = 11.84 VGPRR392 pKa = 11.84 AAPYY396 pKa = 7.17 HH397 pKa = 5.07 TRR399 pKa = 11.84 RR400 pKa = 11.84 AGG402 pKa = 3.07
Molecular weight: 43.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.483
IPC2_protein 5.512
IPC_protein 5.474
Toseland 5.575
ProMoST 5.664
Dawson 5.525
Bjellqvist 5.626
Wikipedia 5.423
Rodwell 5.474
Grimsley 5.575
Solomon 5.525
Lehninger 5.499
Nozaki 5.69
DTASelect 5.83
Thurlkill 5.639
EMBOSS 5.575
Sillero 5.779
Patrickios 4.546
IPC_peptide 5.537
IPC2_peptide 5.792
IPC2.peptide.svr19 5.792
Protein with the highest isoelectric point:
>tr|A0A2Z6JIN0|A0A2Z6JIN0_9VIRU ORF1p OS=Cleome droserifolia amalgavirus 1 OX=2069321 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.92 AEE3 pKa = 4.17 HH4 pKa = 6.88 QDD6 pKa = 3.52 PQAQGGAVGGDD17 pKa = 3.38 VEE19 pKa = 4.85 EE20 pKa = 4.5 VNLLEE25 pKa = 5.01 GIGGIPTAAEE35 pKa = 3.7 EE36 pKa = 4.07 RR37 pKa = 11.84 NRR39 pKa = 11.84 LQEE42 pKa = 3.95 ALRR45 pKa = 11.84 PLIANGVNIEE55 pKa = 4.39 RR56 pKa = 11.84 ITVNDD61 pKa = 3.94 CLRR64 pKa = 11.84 LGFTVEE70 pKa = 3.68 QLARR74 pKa = 11.84 AIRR77 pKa = 11.84 VLTSIASDD85 pKa = 3.43 EE86 pKa = 4.04 MRR88 pKa = 11.84 DD89 pKa = 3.83 LIFSQAHH96 pKa = 6.53 LSRR99 pKa = 11.84 LAPAARR105 pKa = 11.84 SVTLDD110 pKa = 3.79 HH111 pKa = 7.11 ILAICEE117 pKa = 3.97 WMKK120 pKa = 10.15 TEE122 pKa = 5.42 RR123 pKa = 11.84 GAQAIRR129 pKa = 11.84 NLQTSAKK136 pKa = 7.74 QEE138 pKa = 3.61 RR139 pKa = 11.84 RR140 pKa = 11.84 AVGGRR145 pKa = 11.84 TVADD149 pKa = 3.35 VSMLQAFNQQVADD162 pKa = 3.88 WSAQVKK168 pKa = 7.77 EE169 pKa = 3.91 ARR171 pKa = 11.84 KK172 pKa = 9.78 EE173 pKa = 3.89 IEE175 pKa = 3.87 EE176 pKa = 4.03 EE177 pKa = 4.03 MAEE180 pKa = 4.73 LRR182 pKa = 11.84 AQLALKK188 pKa = 9.51 QQEE191 pKa = 4.71 LNDD194 pKa = 3.53 SLAAINEE201 pKa = 4.68 SYY203 pKa = 9.91 WPASSYY209 pKa = 10.33 KK210 pKa = 10.5 APALNVVQSQAYY222 pKa = 8.87 ALYY225 pKa = 10.54 AADD228 pKa = 4.26 CRR230 pKa = 11.84 RR231 pKa = 11.84 SGTVPLPAGSLGYY244 pKa = 10.37 SKK246 pKa = 10.83 AVEE249 pKa = 4.04 LFGQQVRR256 pKa = 11.84 EE257 pKa = 4.05 DD258 pKa = 3.58 HH259 pKa = 5.63 QLQYY263 pKa = 11.34 LRR265 pKa = 11.84 AEE267 pKa = 4.45 EE268 pKa = 4.64 ARR270 pKa = 11.84 DD271 pKa = 3.56 TLSEE275 pKa = 4.03 YY276 pKa = 10.66 LQRR279 pKa = 11.84 LVGLKK284 pKa = 10.1 AGQGRR289 pKa = 11.84 RR290 pKa = 11.84 RR291 pKa = 11.84 EE292 pKa = 4.12 VEE294 pKa = 3.73 NFVPSWQEE302 pKa = 3.36 WVSQLLMRR310 pKa = 11.84 YY311 pKa = 8.36 PLEE314 pKa = 3.95 RR315 pKa = 11.84 RR316 pKa = 11.84 RR317 pKa = 11.84 WLLNRR322 pKa = 11.84 LVVGRR327 pKa = 11.84 INPPGRR333 pKa = 11.84 EE334 pKa = 3.55 PRR336 pKa = 11.84 TRR338 pKa = 11.84 LLRR341 pKa = 11.84 EE342 pKa = 3.85 VMPAEE347 pKa = 3.88 LMKK350 pKa = 10.71 AKK352 pKa = 9.74 RR353 pKa = 11.84 QAGIRR358 pKa = 11.84 HH359 pKa = 5.81 RR360 pKa = 11.84 PGLQRR365 pKa = 11.84 KK366 pKa = 8.84 IEE368 pKa = 4.01 EE369 pKa = 4.37 AGGGSRR375 pKa = 11.84 GAEE378 pKa = 3.46 MTGRR382 pKa = 11.84 VRR384 pKa = 11.84 VLVEE388 pKa = 3.65 WGRR391 pKa = 11.84 EE392 pKa = 3.66 RR393 pKa = 11.84 HH394 pKa = 4.74 RR395 pKa = 11.84 TIPVARR401 pKa = 11.84 GKK403 pKa = 10.73 FEE405 pKa = 4.31 AGIRR409 pKa = 11.84 KK410 pKa = 9.56 IIGGGEE416 pKa = 4.04 LLNWRR421 pKa = 11.84 EE422 pKa = 4.05 DD423 pKa = 3.09 SSMYY427 pKa = 10.44 RR428 pKa = 11.84 GGGNSADD435 pKa = 4.43 AIRR438 pKa = 11.84 MLFSADD444 pKa = 4.06 PIPPGRR450 pKa = 11.84 VLTDD454 pKa = 3.62 FWRR457 pKa = 11.84 EE458 pKa = 3.46 GSARR462 pKa = 11.84 QALFIEE468 pKa = 4.73 EE469 pKa = 4.47 LFEE472 pKa = 4.58 VPDD475 pKa = 4.4 GPGCCVMKK483 pKa = 10.78 NFNNEE488 pKa = 3.94 ATSGPYY494 pKa = 10.47 LRR496 pKa = 11.84 GQGLKK501 pKa = 10.33 GKK503 pKa = 10.02 YY504 pKa = 7.91 GLKK507 pKa = 10.23 RR508 pKa = 11.84 ILEE511 pKa = 4.35 AEE513 pKa = 3.72 MWRR516 pKa = 11.84 YY517 pKa = 9.68 YY518 pKa = 11.22 DD519 pKa = 3.54 SFASGEE525 pKa = 4.31 CGLKK529 pKa = 10.0 NLPWFAARR537 pKa = 11.84 VGFRR541 pKa = 11.84 TKK543 pKa = 11.04 LLTQEE548 pKa = 4.19 VAWAKK553 pKa = 6.78 MQKK556 pKa = 10.6 GEE558 pKa = 4.06 ALGRR562 pKa = 11.84 AVMMMDD568 pKa = 4.21 ALEE571 pKa = 4.12 QAASSPMYY579 pKa = 10.33 NVLSSYY585 pKa = 7.1 TFRR588 pKa = 11.84 RR589 pKa = 11.84 RR590 pKa = 11.84 LEE592 pKa = 3.83 PRR594 pKa = 11.84 CGFKK598 pKa = 10.8 NGVVRR603 pKa = 11.84 ASSDD607 pKa = 2.79 WGVLWRR613 pKa = 11.84 RR614 pKa = 11.84 VQQAKK619 pKa = 10.68 VIVEE623 pKa = 4.15 LDD625 pKa = 2.81 WSKK628 pKa = 10.98 FDD630 pKa = 3.95 RR631 pKa = 11.84 EE632 pKa = 4.19 RR633 pKa = 11.84 PRR635 pKa = 11.84 EE636 pKa = 3.94 DD637 pKa = 3.67 LEE639 pKa = 5.05 FIVKK643 pKa = 7.94 VVCSCFRR650 pKa = 11.84 PRR652 pKa = 11.84 DD653 pKa = 3.6 DD654 pKa = 3.48 RR655 pKa = 11.84 GRR657 pKa = 11.84 RR658 pKa = 11.84 LLQAYY663 pKa = 9.23 QEE665 pKa = 4.15 MMRR668 pKa = 11.84 RR669 pKa = 11.84 ALVEE673 pKa = 3.66 RR674 pKa = 11.84 ALITDD679 pKa = 3.58 SGGVFLVDD687 pKa = 3.21 GMVPSGSLWTGWIDD701 pKa = 3.2 TALNILYY708 pKa = 9.89 IKK710 pKa = 9.68 AICVEE715 pKa = 4.21 LGIALSAVEE724 pKa = 4.43 VFCAGDD730 pKa = 3.68 DD731 pKa = 3.78 NLTLFMYY738 pKa = 10.55 DD739 pKa = 3.45 PGDD742 pKa = 3.84 VILKK746 pKa = 8.24 EE747 pKa = 4.12 FRR749 pKa = 11.84 TLLNQYY755 pKa = 8.8 FRR757 pKa = 11.84 AGIKK761 pKa = 10.37 EE762 pKa = 3.9 EE763 pKa = 4.45 DD764 pKa = 4.15 FFIHH768 pKa = 7.05 RR769 pKa = 11.84 PPFDD773 pKa = 3.35 VRR775 pKa = 11.84 KK776 pKa = 8.81 YY777 pKa = 9.83 QATFPVGSDD786 pKa = 3.18 LRR788 pKa = 11.84 GGTSRR793 pKa = 11.84 MLDD796 pKa = 2.86 RR797 pKa = 11.84 ATWIEE802 pKa = 3.92 FEE804 pKa = 4.47 GEE806 pKa = 4.04 IEE808 pKa = 3.89 VDD810 pKa = 3.34 EE811 pKa = 4.5 ARR813 pKa = 11.84 GRR815 pKa = 11.84 SHH817 pKa = 6.47 RR818 pKa = 11.84 WEE820 pKa = 3.77 YY821 pKa = 10.77 RR822 pKa = 11.84 FKK824 pKa = 11.1 GKK826 pKa = 9.59 PKK828 pKa = 10.17 FLSNYY833 pKa = 6.81 WLPDD837 pKa = 3.23 GRR839 pKa = 11.84 PVRR842 pKa = 11.84 PTRR845 pKa = 11.84 DD846 pKa = 3.54 NLEE849 pKa = 3.98 KK850 pKa = 10.83 LLWPEE855 pKa = 4.14 GVHH858 pKa = 6.16 ATLEE862 pKa = 4.39 DD863 pKa = 3.71 YY864 pKa = 10.69 EE865 pKa = 4.66 GAVISMVVDD874 pKa = 3.37 NPFNHH879 pKa = 6.7 HH880 pKa = 6.04 NVNHH884 pKa = 5.88 MLMRR888 pKa = 11.84 YY889 pKa = 9.68 VIIQQLRR896 pKa = 11.84 RR897 pKa = 11.84 MSVAVGEE904 pKa = 4.41 LDD906 pKa = 5.03 LLLKK910 pKa = 10.37 LGKK913 pKa = 9.67 IRR915 pKa = 11.84 AKK917 pKa = 10.52 GDD919 pKa = 3.41 EE920 pKa = 4.71 EE921 pKa = 5.2 VPMPQIAPWRR931 pKa = 11.84 RR932 pKa = 11.84 GEE934 pKa = 4.16 LQAKK938 pKa = 8.83 MEE940 pKa = 5.39 DD941 pKa = 3.71 YY942 pKa = 11.47 NDD944 pKa = 3.91 AQRR947 pKa = 11.84 HH948 pKa = 4.59 LAVFEE953 pKa = 4.33 DD954 pKa = 4.45 FMRR957 pKa = 11.84 GVSTLYY963 pKa = 10.69 SRR965 pKa = 11.84 APEE968 pKa = 4.7 GGLDD972 pKa = 2.8 AWQITDD978 pKa = 2.8 IMRR981 pKa = 11.84 GMGSVGEE988 pKa = 4.18 GQFGNEE994 pKa = 3.73 TLSWLKK1000 pKa = 10.04 WMHH1003 pKa = 5.88 HH1004 pKa = 6.46 HH1005 pKa = 7.28 PLTRR1009 pKa = 11.84 YY1010 pKa = 9.09 LRR1012 pKa = 11.84 AARR1015 pKa = 11.84 RR1016 pKa = 11.84 YY1017 pKa = 8.64 QDD1019 pKa = 2.85 PSMPVEE1025 pKa = 4.41 LEE1027 pKa = 3.58 PSQRR1031 pKa = 11.84 AEE1033 pKa = 3.64 ALRR1036 pKa = 11.84 AFEE1039 pKa = 4.16 ALRR1042 pKa = 11.84 GRR1044 pKa = 11.84 LLYY1047 pKa = 10.85 DD1048 pKa = 3.55 GFASSEE1054 pKa = 4.25 EE1055 pKa = 3.9 YY1056 pKa = 10.69 AIWVARR1062 pKa = 11.84 LVKK1065 pKa = 10.22 LRR1067 pKa = 11.84 YY1068 pKa = 8.9 LAA1070 pKa = 4.62
Molecular weight: 121.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.038
IPC2_protein 8.156
IPC_protein 8.053
Toseland 8.214
ProMoST 8.58
Dawson 8.858
Bjellqvist 8.946
Wikipedia 8.931
Rodwell 8.916
Grimsley 8.39
Solomon 8.916
Lehninger 8.902
Nozaki 9.033
DTASelect 8.785
Thurlkill 8.843
EMBOSS 9.004
Sillero 9.077
Patrickios 4.762
IPC_peptide 8.916
IPC2_peptide 7.761
IPC2.peptide.svr19 7.792
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1472
402
1070
736.0
82.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.005 ± 2.247
0.951 ± 0.099
4.755 ± 0.106
8.56 ± 0.31
2.853 ± 0.775
8.492 ± 0.463
1.495 ± 0.001
4.076 ± 0.166
3.193 ± 0.579
9.715 ± 0.725
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.717 ± 0.59
2.921 ± 0.089
4.348 ± 0.062
5.163 ± 0.868
10.462 ± 0.007
4.891 ± 0.199
3.125 ± 0.172
6.658 ± 0.091
1.97 ± 0.589
2.649 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here