Cleome droserifolia amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z6JIN0|A0A2Z6JIN0_9VIRU ORF1p OS=Cleome droserifolia amalgavirus 1 OX=2069321 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.17HH4 pKa = 6.88QDD6 pKa = 3.52PQAQGGAVGGDD17 pKa = 3.38VEE19 pKa = 4.85EE20 pKa = 4.5VNLLEE25 pKa = 5.01GIGGIPTAAEE35 pKa = 3.7EE36 pKa = 4.07RR37 pKa = 11.84NRR39 pKa = 11.84LQEE42 pKa = 3.95ALRR45 pKa = 11.84PLIANGVNIEE55 pKa = 4.39RR56 pKa = 11.84ITVNDD61 pKa = 3.94CLRR64 pKa = 11.84LGFTVEE70 pKa = 3.68QLARR74 pKa = 11.84AIRR77 pKa = 11.84VLTSIASDD85 pKa = 3.43EE86 pKa = 4.04MRR88 pKa = 11.84DD89 pKa = 3.83LIFSQAHH96 pKa = 6.53LSRR99 pKa = 11.84LAPAARR105 pKa = 11.84SVTLDD110 pKa = 3.79HH111 pKa = 7.11ILAICEE117 pKa = 3.97WMKK120 pKa = 10.15TEE122 pKa = 5.42RR123 pKa = 11.84GAQAIRR129 pKa = 11.84NLQTSAKK136 pKa = 7.74QEE138 pKa = 3.61RR139 pKa = 11.84RR140 pKa = 11.84AVGGRR145 pKa = 11.84TVADD149 pKa = 3.35VSMLQAFNQQVADD162 pKa = 3.88WSAQVKK168 pKa = 7.77EE169 pKa = 3.91ARR171 pKa = 11.84KK172 pKa = 9.78EE173 pKa = 3.89IEE175 pKa = 3.87EE176 pKa = 4.03EE177 pKa = 4.03MAEE180 pKa = 4.73LRR182 pKa = 11.84AQLALKK188 pKa = 9.51QQEE191 pKa = 4.71LNDD194 pKa = 3.53SLAAINEE201 pKa = 4.68SYY203 pKa = 9.91WPASSYY209 pKa = 10.33KK210 pKa = 10.5APALNVVQSQAYY222 pKa = 8.87ALYY225 pKa = 10.54AADD228 pKa = 4.26CRR230 pKa = 11.84RR231 pKa = 11.84SGTVPLPAGSLGYY244 pKa = 10.37SKK246 pKa = 10.83AVEE249 pKa = 4.04LFGQQVRR256 pKa = 11.84EE257 pKa = 4.05DD258 pKa = 3.58HH259 pKa = 5.63QLQYY263 pKa = 11.34LRR265 pKa = 11.84AEE267 pKa = 4.45EE268 pKa = 4.64ARR270 pKa = 11.84DD271 pKa = 3.56TLSEE275 pKa = 4.03YY276 pKa = 10.66LQRR279 pKa = 11.84LVGLKK284 pKa = 10.1AGQGRR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84EE292 pKa = 4.23VEE294 pKa = 3.66NFRR297 pKa = 11.84AILAGMGVAAADD309 pKa = 3.74AVPARR314 pKa = 11.84AEE316 pKa = 3.94EE317 pKa = 4.12VAIEE321 pKa = 3.99QVGGGEE327 pKa = 4.11DD328 pKa = 3.31QPAGEE333 pKa = 4.68RR334 pKa = 11.84APDD337 pKa = 3.48QAPEE341 pKa = 4.36GGDD344 pKa = 3.03AGGADD349 pKa = 3.84EE350 pKa = 5.81GEE352 pKa = 4.28AAGGHH357 pKa = 4.43QTPPRR362 pKa = 11.84PAAEE366 pKa = 4.02DD367 pKa = 3.3RR368 pKa = 11.84RR369 pKa = 11.84GGRR372 pKa = 11.84RR373 pKa = 11.84VTRR376 pKa = 11.84RR377 pKa = 11.84RR378 pKa = 11.84DD379 pKa = 3.39DD380 pKa = 3.59GAGPSTRR387 pKa = 11.84RR388 pKa = 11.84VGPRR392 pKa = 11.84AAPYY396 pKa = 7.17HH397 pKa = 5.07TRR399 pKa = 11.84RR400 pKa = 11.84AGG402 pKa = 3.07

Molecular weight:
43.43 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z6JIN0|A0A2Z6JIN0_9VIRU ORF1p OS=Cleome droserifolia amalgavirus 1 OX=2069321 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.17HH4 pKa = 6.88QDD6 pKa = 3.52PQAQGGAVGGDD17 pKa = 3.38VEE19 pKa = 4.85EE20 pKa = 4.5VNLLEE25 pKa = 5.01GIGGIPTAAEE35 pKa = 3.7EE36 pKa = 4.07RR37 pKa = 11.84NRR39 pKa = 11.84LQEE42 pKa = 3.95ALRR45 pKa = 11.84PLIANGVNIEE55 pKa = 4.39RR56 pKa = 11.84ITVNDD61 pKa = 3.94CLRR64 pKa = 11.84LGFTVEE70 pKa = 3.68QLARR74 pKa = 11.84AIRR77 pKa = 11.84VLTSIASDD85 pKa = 3.43EE86 pKa = 4.04MRR88 pKa = 11.84DD89 pKa = 3.83LIFSQAHH96 pKa = 6.53LSRR99 pKa = 11.84LAPAARR105 pKa = 11.84SVTLDD110 pKa = 3.79HH111 pKa = 7.11ILAICEE117 pKa = 3.97WMKK120 pKa = 10.15TEE122 pKa = 5.42RR123 pKa = 11.84GAQAIRR129 pKa = 11.84NLQTSAKK136 pKa = 7.74QEE138 pKa = 3.61RR139 pKa = 11.84RR140 pKa = 11.84AVGGRR145 pKa = 11.84TVADD149 pKa = 3.35VSMLQAFNQQVADD162 pKa = 3.88WSAQVKK168 pKa = 7.77EE169 pKa = 3.91ARR171 pKa = 11.84KK172 pKa = 9.78EE173 pKa = 3.89IEE175 pKa = 3.87EE176 pKa = 4.03EE177 pKa = 4.03MAEE180 pKa = 4.73LRR182 pKa = 11.84AQLALKK188 pKa = 9.51QQEE191 pKa = 4.71LNDD194 pKa = 3.53SLAAINEE201 pKa = 4.68SYY203 pKa = 9.91WPASSYY209 pKa = 10.33KK210 pKa = 10.5APALNVVQSQAYY222 pKa = 8.87ALYY225 pKa = 10.54AADD228 pKa = 4.26CRR230 pKa = 11.84RR231 pKa = 11.84SGTVPLPAGSLGYY244 pKa = 10.37SKK246 pKa = 10.83AVEE249 pKa = 4.04LFGQQVRR256 pKa = 11.84EE257 pKa = 4.05DD258 pKa = 3.58HH259 pKa = 5.63QLQYY263 pKa = 11.34LRR265 pKa = 11.84AEE267 pKa = 4.45EE268 pKa = 4.64ARR270 pKa = 11.84DD271 pKa = 3.56TLSEE275 pKa = 4.03YY276 pKa = 10.66LQRR279 pKa = 11.84LVGLKK284 pKa = 10.1AGQGRR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84EE292 pKa = 4.12VEE294 pKa = 3.73NFVPSWQEE302 pKa = 3.36WVSQLLMRR310 pKa = 11.84YY311 pKa = 8.36PLEE314 pKa = 3.95RR315 pKa = 11.84RR316 pKa = 11.84RR317 pKa = 11.84WLLNRR322 pKa = 11.84LVVGRR327 pKa = 11.84INPPGRR333 pKa = 11.84EE334 pKa = 3.55PRR336 pKa = 11.84TRR338 pKa = 11.84LLRR341 pKa = 11.84EE342 pKa = 3.85VMPAEE347 pKa = 3.88LMKK350 pKa = 10.71AKK352 pKa = 9.74RR353 pKa = 11.84QAGIRR358 pKa = 11.84HH359 pKa = 5.81RR360 pKa = 11.84PGLQRR365 pKa = 11.84KK366 pKa = 8.84IEE368 pKa = 4.01EE369 pKa = 4.37AGGGSRR375 pKa = 11.84GAEE378 pKa = 3.46MTGRR382 pKa = 11.84VRR384 pKa = 11.84VLVEE388 pKa = 3.65WGRR391 pKa = 11.84EE392 pKa = 3.66RR393 pKa = 11.84HH394 pKa = 4.74RR395 pKa = 11.84TIPVARR401 pKa = 11.84GKK403 pKa = 10.73FEE405 pKa = 4.31AGIRR409 pKa = 11.84KK410 pKa = 9.56IIGGGEE416 pKa = 4.04LLNWRR421 pKa = 11.84EE422 pKa = 4.05DD423 pKa = 3.09SSMYY427 pKa = 10.44RR428 pKa = 11.84GGGNSADD435 pKa = 4.43AIRR438 pKa = 11.84MLFSADD444 pKa = 4.06PIPPGRR450 pKa = 11.84VLTDD454 pKa = 3.62FWRR457 pKa = 11.84EE458 pKa = 3.46GSARR462 pKa = 11.84QALFIEE468 pKa = 4.73EE469 pKa = 4.47LFEE472 pKa = 4.58VPDD475 pKa = 4.4GPGCCVMKK483 pKa = 10.78NFNNEE488 pKa = 3.94ATSGPYY494 pKa = 10.47LRR496 pKa = 11.84GQGLKK501 pKa = 10.33GKK503 pKa = 10.02YY504 pKa = 7.91GLKK507 pKa = 10.23RR508 pKa = 11.84ILEE511 pKa = 4.35AEE513 pKa = 3.72MWRR516 pKa = 11.84YY517 pKa = 9.68YY518 pKa = 11.22DD519 pKa = 3.54SFASGEE525 pKa = 4.31CGLKK529 pKa = 10.0NLPWFAARR537 pKa = 11.84VGFRR541 pKa = 11.84TKK543 pKa = 11.04LLTQEE548 pKa = 4.19VAWAKK553 pKa = 6.78MQKK556 pKa = 10.6GEE558 pKa = 4.06ALGRR562 pKa = 11.84AVMMMDD568 pKa = 4.21ALEE571 pKa = 4.12QAASSPMYY579 pKa = 10.33NVLSSYY585 pKa = 7.1TFRR588 pKa = 11.84RR589 pKa = 11.84RR590 pKa = 11.84LEE592 pKa = 3.83PRR594 pKa = 11.84CGFKK598 pKa = 10.8NGVVRR603 pKa = 11.84ASSDD607 pKa = 2.79WGVLWRR613 pKa = 11.84RR614 pKa = 11.84VQQAKK619 pKa = 10.68VIVEE623 pKa = 4.15LDD625 pKa = 2.81WSKK628 pKa = 10.98FDD630 pKa = 3.95RR631 pKa = 11.84EE632 pKa = 4.19RR633 pKa = 11.84PRR635 pKa = 11.84EE636 pKa = 3.94DD637 pKa = 3.67LEE639 pKa = 5.05FIVKK643 pKa = 7.94VVCSCFRR650 pKa = 11.84PRR652 pKa = 11.84DD653 pKa = 3.6DD654 pKa = 3.48RR655 pKa = 11.84GRR657 pKa = 11.84RR658 pKa = 11.84LLQAYY663 pKa = 9.23QEE665 pKa = 4.15MMRR668 pKa = 11.84RR669 pKa = 11.84ALVEE673 pKa = 3.66RR674 pKa = 11.84ALITDD679 pKa = 3.58SGGVFLVDD687 pKa = 3.21GMVPSGSLWTGWIDD701 pKa = 3.2TALNILYY708 pKa = 9.89IKK710 pKa = 9.68AICVEE715 pKa = 4.21LGIALSAVEE724 pKa = 4.43VFCAGDD730 pKa = 3.68DD731 pKa = 3.78NLTLFMYY738 pKa = 10.55DD739 pKa = 3.45PGDD742 pKa = 3.84VILKK746 pKa = 8.24EE747 pKa = 4.12FRR749 pKa = 11.84TLLNQYY755 pKa = 8.8FRR757 pKa = 11.84AGIKK761 pKa = 10.37EE762 pKa = 3.9EE763 pKa = 4.45DD764 pKa = 4.15FFIHH768 pKa = 7.05RR769 pKa = 11.84PPFDD773 pKa = 3.35VRR775 pKa = 11.84KK776 pKa = 8.81YY777 pKa = 9.83QATFPVGSDD786 pKa = 3.18LRR788 pKa = 11.84GGTSRR793 pKa = 11.84MLDD796 pKa = 2.86RR797 pKa = 11.84ATWIEE802 pKa = 3.92FEE804 pKa = 4.47GEE806 pKa = 4.04IEE808 pKa = 3.89VDD810 pKa = 3.34EE811 pKa = 4.5ARR813 pKa = 11.84GRR815 pKa = 11.84SHH817 pKa = 6.47RR818 pKa = 11.84WEE820 pKa = 3.77YY821 pKa = 10.77RR822 pKa = 11.84FKK824 pKa = 11.1GKK826 pKa = 9.59PKK828 pKa = 10.17FLSNYY833 pKa = 6.81WLPDD837 pKa = 3.23GRR839 pKa = 11.84PVRR842 pKa = 11.84PTRR845 pKa = 11.84DD846 pKa = 3.54NLEE849 pKa = 3.98KK850 pKa = 10.83LLWPEE855 pKa = 4.14GVHH858 pKa = 6.16ATLEE862 pKa = 4.39DD863 pKa = 3.71YY864 pKa = 10.69EE865 pKa = 4.66GAVISMVVDD874 pKa = 3.37NPFNHH879 pKa = 6.7HH880 pKa = 6.04NVNHH884 pKa = 5.88MLMRR888 pKa = 11.84YY889 pKa = 9.68VIIQQLRR896 pKa = 11.84RR897 pKa = 11.84MSVAVGEE904 pKa = 4.41LDD906 pKa = 5.03LLLKK910 pKa = 10.37LGKK913 pKa = 9.67IRR915 pKa = 11.84AKK917 pKa = 10.52GDD919 pKa = 3.41EE920 pKa = 4.71EE921 pKa = 5.2VPMPQIAPWRR931 pKa = 11.84RR932 pKa = 11.84GEE934 pKa = 4.16LQAKK938 pKa = 8.83MEE940 pKa = 5.39DD941 pKa = 3.71YY942 pKa = 11.47NDD944 pKa = 3.91AQRR947 pKa = 11.84HH948 pKa = 4.59LAVFEE953 pKa = 4.33DD954 pKa = 4.45FMRR957 pKa = 11.84GVSTLYY963 pKa = 10.69SRR965 pKa = 11.84APEE968 pKa = 4.7GGLDD972 pKa = 2.8AWQITDD978 pKa = 2.8IMRR981 pKa = 11.84GMGSVGEE988 pKa = 4.18GQFGNEE994 pKa = 3.73TLSWLKK1000 pKa = 10.04WMHH1003 pKa = 5.88HH1004 pKa = 6.46HH1005 pKa = 7.28PLTRR1009 pKa = 11.84YY1010 pKa = 9.09LRR1012 pKa = 11.84AARR1015 pKa = 11.84RR1016 pKa = 11.84YY1017 pKa = 8.64QDD1019 pKa = 2.85PSMPVEE1025 pKa = 4.41LEE1027 pKa = 3.58PSQRR1031 pKa = 11.84AEE1033 pKa = 3.64ALRR1036 pKa = 11.84AFEE1039 pKa = 4.16ALRR1042 pKa = 11.84GRR1044 pKa = 11.84LLYY1047 pKa = 10.85DD1048 pKa = 3.55GFASSEE1054 pKa = 4.25EE1055 pKa = 3.9YY1056 pKa = 10.69AIWVARR1062 pKa = 11.84LVKK1065 pKa = 10.22LRR1067 pKa = 11.84YY1068 pKa = 8.9LAA1070 pKa = 4.62

Molecular weight:
121.77 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1472

402

1070

736.0

82.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.005 ± 2.247

0.951 ± 0.099

4.755 ± 0.106

8.56 ± 0.31

2.853 ± 0.775

8.492 ± 0.463

1.495 ± 0.001

4.076 ± 0.166

3.193 ± 0.579

9.715 ± 0.725

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.59

2.921 ± 0.089

4.348 ± 0.062

5.163 ± 0.868

10.462 ± 0.007

4.891 ± 0.199

3.125 ± 0.172

6.658 ± 0.091

1.97 ± 0.589

2.649 ± 0.317

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski