Vibrio phage ValB1MD-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; unclassified Peduovirinae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7F5G8|A0A7D7F5G8_9CAUD Uncharacterized protein OS=Vibrio phage ValB1MD-2 OX=2759177 PE=4 SV=1
MM1 pKa = 5.88MTVYY5 pKa = 10.74KK6 pKa = 9.86ITDD9 pKa = 4.06DD10 pKa = 3.46LTKK13 pKa = 10.42FQSVCSGPDD22 pKa = 3.22EE23 pKa = 4.74IATQLGDD30 pKa = 3.08FDD32 pKa = 4.18YY33 pKa = 11.22FEE35 pKa = 6.47RR36 pKa = 11.84ILLQAVEE43 pKa = 4.32NKK45 pKa = 9.69SLKK48 pKa = 10.47DD49 pKa = 3.0IWKK52 pKa = 8.04SAEE55 pKa = 3.87VEE57 pKa = 4.23FEE59 pKa = 5.53DD60 pKa = 4.84VLTDD64 pKa = 3.7NSVLPDD70 pKa = 3.25ISLWLRR76 pKa = 11.84TYY78 pKa = 11.05LVLSPRR84 pKa = 11.84AYY86 pKa = 9.37EE87 pKa = 4.06ALNSFLSEE95 pKa = 4.19SGDD98 pKa = 3.69FLPVNYY104 pKa = 9.81QSEE107 pKa = 3.98QWYY110 pKa = 9.68LYY112 pKa = 10.45TSLTFGKK119 pKa = 10.0EE120 pKa = 3.71DD121 pKa = 3.33RR122 pKa = 11.84QKK124 pKa = 10.49CVEE127 pKa = 4.35KK128 pKa = 10.4ISYY131 pKa = 9.5GSPDD135 pKa = 3.63GLEE138 pKa = 3.72VLAFIDD144 pKa = 5.47DD145 pKa = 3.92DD146 pKa = 4.51VKK148 pKa = 11.57DD149 pKa = 3.67KK150 pKa = 11.6VIFKK154 pKa = 10.67SRR156 pKa = 11.84LEE158 pKa = 4.28GASNLYY164 pKa = 8.36CTEE167 pKa = 3.96RR168 pKa = 11.84FKK170 pKa = 11.7AMCEE174 pKa = 3.83QNQLNGLVFSSNLTDD189 pKa = 4.36PFDD192 pKa = 3.46

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7IM00|A0A7D7IM00_9CAUD AAA_15 domain-containing protein OS=Vibrio phage ValB1MD-2 OX=2759177 PE=4 SV=1
MM1 pKa = 7.79YY2 pKa = 9.37EE3 pKa = 3.24IRR5 pKa = 11.84ADD7 pKa = 3.17KK8 pKa = 10.55RR9 pKa = 11.84SYY11 pKa = 11.24LRR13 pKa = 11.84VKK15 pKa = 10.29EE16 pKa = 3.9QFEE19 pKa = 4.14LLKK22 pKa = 10.84LEE24 pKa = 4.3KK25 pKa = 9.98KK26 pKa = 10.48ARR28 pKa = 11.84ARR30 pKa = 11.84VLKK33 pKa = 10.43EE34 pKa = 3.29LGKK37 pKa = 11.06YY38 pKa = 7.22ITKK41 pKa = 6.98TTKK44 pKa = 10.41KK45 pKa = 10.16NIRR48 pKa = 11.84AQRR51 pKa = 11.84DD52 pKa = 3.4PDD54 pKa = 3.35GKK56 pKa = 10.61AWSKK60 pKa = 10.64RR61 pKa = 11.84KK62 pKa = 9.51NGRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.57MLKK70 pKa = 10.5GFTKK74 pKa = 10.46KK75 pKa = 10.26LKK77 pKa = 10.52HH78 pKa = 5.31FQKK81 pKa = 10.9DD82 pKa = 3.37NNRR85 pKa = 11.84VLVVGWPSRR94 pKa = 11.84RR95 pKa = 11.84GTVALAHH102 pKa = 5.89HH103 pKa = 6.49TGEE106 pKa = 4.39AEE108 pKa = 4.25EE109 pKa = 5.19SGLQQRR115 pKa = 11.84FKK117 pKa = 10.52QAKK120 pKa = 8.23KK121 pKa = 10.21AKK123 pKa = 8.68EE124 pKa = 3.95PKK126 pKa = 9.1KK127 pKa = 8.92TDD129 pKa = 3.3PATRR133 pKa = 11.84EE134 pKa = 3.76QAKK137 pKa = 8.99EE138 pKa = 3.79LRR140 pKa = 11.84DD141 pKa = 3.35LGYY144 pKa = 10.69RR145 pKa = 11.84LPPQGRR151 pKa = 11.84QKK153 pKa = 10.54RR154 pKa = 11.84GKK156 pKa = 10.16KK157 pKa = 7.91PTLKK161 pKa = 10.7FITQNMTVAEE171 pKa = 4.12AAKK174 pKa = 10.44LISDD178 pKa = 5.26LEE180 pKa = 4.33NKK182 pKa = 8.88TPSRR186 pKa = 11.84KK187 pKa = 9.04WEE189 pKa = 3.57VDD191 pKa = 3.03RR192 pKa = 11.84PEE194 pKa = 3.91RR195 pKa = 11.84RR196 pKa = 11.84LIGISPKK203 pKa = 9.58RR204 pKa = 11.84AAMIIKK210 pKa = 10.11RR211 pKa = 11.84EE212 pKa = 4.02MNRR215 pKa = 11.84NRR217 pKa = 11.84SNN219 pKa = 3.01

Molecular weight: 25.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10989

66

695

249.8

28.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.072 ± 0.468

1.01 ± 0.12

5.506 ± 0.222

6.971 ± 0.325

3.886 ± 0.236

5.842 ± 0.346

2.239 ± 0.219

5.924 ± 0.26

7.298 ± 0.397

9.118 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.214

4.841 ± 0.233

3.949 ± 0.209

4.359 ± 0.224

4.905 ± 0.307

6.871 ± 0.303

5.933 ± 0.307

6.315 ± 0.363

1.565 ± 0.117

3.003 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski