Segniliparus rotundus (strain ATCC BAA-972 / CDC 1076 / CIP 108378 / DSM 44985 / JCM 13578)
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3001 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6ZDK6|D6ZDK6_SEGRD Uncharacterized protein OS=Segniliparus rotundus (strain ATCC BAA-972 / CDC 1076 / CIP 108378 / DSM 44985 / JCM 13578) OX=640132 GN=Srot_2832 PE=4 SV=1
MM1 pKa = 7.79 SDD3 pKa = 3.02 NADD6 pKa = 3.4 YY7 pKa = 11.06 KK8 pKa = 11.19 LYY10 pKa = 9.94 MCLQCGFEE18 pKa = 4.14 YY19 pKa = 10.88 DD20 pKa = 4.01 EE21 pKa = 4.49 ALGWPEE27 pKa = 5.22 DD28 pKa = 4.3 GIAPGTRR35 pKa = 11.84 WDD37 pKa = 5.73 DD38 pKa = 4.03 IPDD41 pKa = 3.65 DD42 pKa = 4.18 WTCPDD47 pKa = 4.12 CGATKK52 pKa = 10.7 ADD54 pKa = 3.51 FEE56 pKa = 4.5 MVEE59 pKa = 4.49 KK60 pKa = 10.9 VRR62 pKa = 11.84 SS63 pKa = 3.51
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.528
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|D6ZEN2|D6ZEN2_SEGRD Transcriptional regulator XRE family OS=Segniliparus rotundus (strain ATCC BAA-972 / CDC 1076 / CIP 108378 / DSM 44985 / JCM 13578) OX=640132 GN=Srot_0932 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 9.89 VSGFRR22 pKa = 11.84 VRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AVLASRR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.18 GRR42 pKa = 11.84 VRR44 pKa = 11.84 ISAA47 pKa = 3.58
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3001
0
3001
945808
31
3073
315.2
33.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.555 ± 0.062
0.859 ± 0.013
5.596 ± 0.036
5.9 ± 0.045
3.192 ± 0.026
8.927 ± 0.042
2.225 ± 0.023
3.612 ± 0.03
2.934 ± 0.033
10.023 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.755 ± 0.018
2.152 ± 0.021
5.891 ± 0.043
3.561 ± 0.028
7.072 ± 0.05
5.866 ± 0.034
4.992 ± 0.023
8.334 ± 0.038
1.569 ± 0.02
1.983 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here