Tropilaelaps mercedesae
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14292 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V9XYY5|A0A1V9XYY5_9ACAR Methenyltetrahydrofolate cyclohydrolase OS=Tropilaelaps mercedesae OX=418985 GN=BIW11_00262 PE=3 SV=1
MM1 pKa = 7.46 SRR3 pKa = 11.84 QEE5 pKa = 4.46 DD6 pKa = 3.65 SDD8 pKa = 4.47 DD9 pKa = 3.49 GVMPVALSDD18 pKa = 4.02 SDD20 pKa = 3.98 WEE22 pKa = 4.05 RR23 pKa = 11.84 LSYY26 pKa = 11.2 DD27 pKa = 4.2 EE28 pKa = 6.11 DD29 pKa = 3.86 SQPPSLDD36 pKa = 3.43 PDD38 pKa = 3.98 LEE40 pKa = 4.91 AGPGEE45 pKa = 4.25 YY46 pKa = 9.88 EE47 pKa = 4.24 EE48 pKa = 5.5 EE49 pKa = 5.13 DD50 pKa = 3.18 IAPCEE55 pKa = 3.98 EE56 pKa = 5.32 DD57 pKa = 3.43 PLEE60 pKa = 5.51 DD61 pKa = 3.36 EE62 pKa = 5.39 GEE64 pKa = 4.27 PANLTAPFDD73 pKa = 4.53 LDD75 pKa = 4.31 DD76 pKa = 4.93 DD77 pKa = 4.73 ASEE80 pKa = 4.29 NVISTEE86 pKa = 3.93 PCDD89 pKa = 3.93 SGILVEE95 pKa = 4.5 RR96 pKa = 11.84 AGARR100 pKa = 11.84 QSSVPFCSSPSQRR113 pKa = 11.84 TGGTGLLLVLICVIQDD129 pKa = 3.48 FLQPRR134 pKa = 11.84 IQWLFDD140 pKa = 3.02 SALYY144 pKa = 10.01 RR145 pKa = 11.84 KK146 pKa = 9.59 YY147 pKa = 10.8 LADD150 pKa = 3.49 NAMLSSYY157 pKa = 10.64 LVTATVVMCLPLAMALLVSALLVACSAAACVLLVAMLVAVGVVLSLVWAFWVFVPVTILLGALFLGSTIYY227 pKa = 10.79 QKK229 pKa = 8.5 TFRR232 pKa = 11.84 IKK234 pKa = 10.72 LSAKK238 pKa = 9.43 EE239 pKa = 3.97 VV240 pKa = 3.41
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.897
Patrickios 1.863
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A1V9WY40|A0A1V9WY40_9ACAR Endophilin-A-like OS=Tropilaelaps mercedesae OX=418985 GN=BIW11_05037 PE=4 SV=1
MM1 pKa = 6.94 SQRR4 pKa = 11.84 VSQRR8 pKa = 11.84 VSQRR12 pKa = 11.84 VSQRR16 pKa = 11.84 VSQKK20 pKa = 9.56 VLQRR24 pKa = 11.84 VPRR27 pKa = 11.84 RR28 pKa = 11.84 LSQRR32 pKa = 11.84 VPQRR36 pKa = 11.84 VLQRR40 pKa = 11.84 VPQRR44 pKa = 11.84 VSHH47 pKa = 6.42 RR48 pKa = 11.84 VLQRR52 pKa = 11.84 VSHH55 pKa = 6.31 RR56 pKa = 11.84 VLQRR60 pKa = 11.84 VPRR63 pKa = 11.84 RR64 pKa = 11.84 LSQRR68 pKa = 11.84 VSQRR72 pKa = 11.84 VPRR75 pKa = 11.84 RR76 pKa = 11.84 WSS78 pKa = 3.01
Molecular weight: 9.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.487
IPC2_protein 11.52
IPC_protein 13.144
Toseland 13.305
ProMoST 13.803
Dawson 13.305
Bjellqvist 13.305
Wikipedia 13.788
Rodwell 12.822
Grimsley 13.334
Solomon 13.803
Lehninger 13.7
Nozaki 13.305
DTASelect 13.305
Thurlkill 13.305
EMBOSS 13.803
Sillero 13.305
Patrickios 12.544
IPC_peptide 13.803
IPC2_peptide 12.793
IPC2.peptide.svr19 9.414
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14272
20
14292
5410289
8
10260
378.6
42.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.77 ± 0.022
2.054 ± 0.019
5.272 ± 0.016
6.111 ± 0.027
3.646 ± 0.015
6.469 ± 0.025
2.676 ± 0.012
4.606 ± 0.017
5.131 ± 0.026
9.248 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.266 ± 0.009
3.954 ± 0.013
5.551 ± 0.027
4.453 ± 0.021
6.434 ± 0.021
8.236 ± 0.028
5.727 ± 0.018
6.648 ± 0.02
1.048 ± 0.007
2.685 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here