Hubei sobemo-like virus 19

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEK7|A0A1L3KEK7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 19 OX=1923204 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 5.43PQPQEE7 pKa = 3.82NNFEE11 pKa = 4.15QEE13 pKa = 4.16EE14 pKa = 4.52LLVQQPILDD23 pKa = 4.15DD24 pKa = 4.07KK25 pKa = 11.12EE26 pKa = 4.74LNLFAKK32 pKa = 9.13MKK34 pKa = 9.17RR35 pKa = 11.84ICVITWRR42 pKa = 11.84SWLAIVHH49 pKa = 6.18LWLYY53 pKa = 10.7ISITKK58 pKa = 10.34YY59 pKa = 9.7LDD61 pKa = 3.02RR62 pKa = 11.84RR63 pKa = 11.84AEE65 pKa = 3.94EE66 pKa = 4.09LKK68 pKa = 10.01MEE70 pKa = 4.75EE71 pKa = 4.45KK72 pKa = 10.0MKK74 pKa = 10.54VWFPVFRR81 pKa = 11.84KK82 pKa = 9.13QLCFIYY88 pKa = 10.36NQLLVATQYY97 pKa = 11.19FDD99 pKa = 4.97AEE101 pKa = 4.69TFVLMLISALLSILTLWLFFKK122 pKa = 10.78KK123 pKa = 9.99SRR125 pKa = 11.84KK126 pKa = 7.34TLMSLRR132 pKa = 11.84GIKK135 pKa = 9.92FEE137 pKa = 4.1AMVEE141 pKa = 4.18GSHH144 pKa = 6.11FVKK147 pKa = 10.76GDD149 pKa = 3.11IPKK152 pKa = 9.97YY153 pKa = 9.92QVAILKK159 pKa = 10.48AGLLRR164 pKa = 11.84DD165 pKa = 3.45VHH167 pKa = 7.82VGFGIRR173 pKa = 11.84VNDD176 pKa = 3.77FLVVPSHH183 pKa = 7.1VINEE187 pKa = 4.16VGNDD191 pKa = 3.59LLLKK195 pKa = 10.63KK196 pKa = 10.25NGKK199 pKa = 7.95IQVSIGQRR207 pKa = 11.84IISDD211 pKa = 3.92YY212 pKa = 11.34VSDD215 pKa = 4.52LAYY218 pKa = 10.81LPISSTVWASLGVTRR233 pKa = 11.84ANLAKK238 pKa = 10.5EE239 pKa = 4.25SLALAAMATVTGNEE253 pKa = 4.13GQTTGLLRR261 pKa = 11.84KK262 pKa = 9.41CAIPFFYY269 pKa = 9.47TYY271 pKa = 9.51TASTVPGMSGAAYY284 pKa = 9.75VSNNMVHH291 pKa = 6.89AVHH294 pKa = 6.53CGVVGEE300 pKa = 4.58HH301 pKa = 6.35NSAISTVVVLKK312 pKa = 10.4EE313 pKa = 3.67LSKK316 pKa = 10.68IMKK319 pKa = 9.64GEE321 pKa = 3.8AVAATSISSNIDD333 pKa = 3.5MQWMPDD339 pKa = 3.21IDD341 pKa = 4.93KK342 pKa = 10.57VWHH345 pKa = 7.09DD346 pKa = 5.2DD347 pKa = 3.38IFDD350 pKa = 3.81KK351 pKa = 11.26YY352 pKa = 11.34VEE354 pKa = 4.24EE355 pKa = 4.63KK356 pKa = 10.78EE357 pKa = 4.14NRR359 pKa = 11.84LLNARR364 pKa = 11.84AKK366 pKa = 10.78HH367 pKa = 5.36GDD369 pKa = 3.28KK370 pKa = 10.99YY371 pKa = 11.35NPPEE375 pKa = 4.05QGWAAMMDD383 pKa = 3.68YY384 pKa = 11.17DD385 pKa = 5.76DD386 pKa = 6.18DD387 pKa = 3.95FAEE390 pKa = 4.84TYY392 pKa = 10.86GEE394 pKa = 4.18SFNVGKK400 pKa = 10.29NPRR403 pKa = 11.84SPMKK407 pKa = 10.71VRR409 pKa = 11.84ITPQGIIDD417 pKa = 4.23SDD419 pKa = 3.89SDD421 pKa = 3.14IDD423 pKa = 4.02VFVQRR428 pKa = 11.84TTDD431 pKa = 3.16AKK433 pKa = 8.37PTRR436 pKa = 11.84RR437 pKa = 11.84LEE439 pKa = 4.21LEE441 pKa = 3.9DD442 pKa = 5.88RR443 pKa = 11.84IANTEE448 pKa = 4.24DD449 pKa = 3.25GTLTNLHH456 pKa = 6.24LRR458 pKa = 11.84IKK460 pKa = 10.51RR461 pKa = 11.84LEE463 pKa = 4.0QKK465 pKa = 10.02VFQVDD470 pKa = 2.87KK471 pKa = 11.48CEE473 pKa = 3.8ICGAVTNQIAKK484 pKa = 9.97HH485 pKa = 5.4MKK487 pKa = 7.92TFHH490 pKa = 6.12GRR492 pKa = 11.84EE493 pKa = 4.1EE494 pKa = 4.27EE495 pKa = 4.1CWPCEE500 pKa = 4.01KK501 pKa = 10.67CSTVCKK507 pKa = 9.7TKK509 pKa = 10.76EE510 pKa = 3.64KK511 pKa = 10.9LEE513 pKa = 4.03RR514 pKa = 11.84HH515 pKa = 5.73LLSHH519 pKa = 6.67PEE521 pKa = 3.86RR522 pKa = 11.84PTCDD526 pKa = 2.59RR527 pKa = 11.84CGITCRR533 pKa = 11.84TQLTLLNHH541 pKa = 7.19RR542 pKa = 11.84NTCLPKK548 pKa = 10.07VAFNKK553 pKa = 8.87KK554 pKa = 6.24TEE556 pKa = 3.95KK557 pKa = 9.17VTLVGEE563 pKa = 4.33SAIPADD569 pKa = 4.05FKK571 pKa = 10.99TVVKK575 pKa = 10.44VDD577 pKa = 3.32KK578 pKa = 11.34NGFLGKK584 pKa = 10.22KK585 pKa = 10.1GSQKK589 pKa = 10.36KK590 pKa = 10.17DD591 pKa = 3.16SRR593 pKa = 11.84SSKK596 pKa = 7.57TTLNLSGEE604 pKa = 4.22KK605 pKa = 10.49DD606 pKa = 3.37PLTSQLEE613 pKa = 4.48FQSEE617 pKa = 4.37MLKK620 pKa = 10.25SQSAMLKK627 pKa = 10.13LLRR630 pKa = 11.84DD631 pKa = 3.96LAQNMNGQNLDD642 pKa = 2.9IMQNN646 pKa = 3.29

Molecular weight: 73.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEK7|A0A1L3KEK7_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 19 OX=1923204 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84GSTTNRR8 pKa = 11.84QLLCYY13 pKa = 9.84KK14 pKa = 10.4NSDD17 pKa = 3.33VFDD20 pKa = 4.02PQKK23 pKa = 10.28IDD25 pKa = 3.53RR26 pKa = 11.84MWEE29 pKa = 3.63IVRR32 pKa = 11.84QKK34 pKa = 10.84LGGSGPDD41 pKa = 3.7PIKK44 pKa = 10.65IFVKK48 pKa = 10.42PEE50 pKa = 3.42PHH52 pKa = 5.96SLKK55 pKa = 10.6KK56 pKa = 10.42ISLKK60 pKa = 10.13RR61 pKa = 11.84YY62 pKa = 8.44RR63 pKa = 11.84LISSVSLADD72 pKa = 3.44QLIDD76 pKa = 3.25HH77 pKa = 6.47MLFGEE82 pKa = 4.49MNDD85 pKa = 3.64KK86 pKa = 10.93LIAGWPYY93 pKa = 10.86LPSKK97 pKa = 10.31VGWSFIGGGWKK108 pKa = 10.43AMPKK112 pKa = 9.77GGSWLAIDD120 pKa = 4.19KK121 pKa = 10.86SSWDD125 pKa = 3.13WTVRR129 pKa = 11.84PWLFDD134 pKa = 3.62LVLEE138 pKa = 4.7ARR140 pKa = 11.84LGLCRR145 pKa = 11.84TRR147 pKa = 11.84GEE149 pKa = 4.48VRR151 pKa = 11.84DD152 pKa = 3.86RR153 pKa = 11.84WSKK156 pKa = 9.67LAVMRR161 pKa = 11.84YY162 pKa = 7.1QQLFGNPIFITSGGFLLKK180 pKa = 10.14QKK182 pKa = 10.63RR183 pKa = 11.84PGVQKK188 pKa = 10.37SGCVNTISDD197 pKa = 3.81NSMMQSLLHH206 pKa = 6.35IRR208 pKa = 11.84VCLEE212 pKa = 3.61TNQPIGVLFSMGDD225 pKa = 3.44DD226 pKa = 3.86TLQQAPPLLDD236 pKa = 4.06EE237 pKa = 4.52YY238 pKa = 11.39LATLSKK244 pKa = 10.97YY245 pKa = 10.73CIVKK249 pKa = 9.83QAGDD253 pKa = 3.6EE254 pKa = 4.33VEE256 pKa = 4.12FAGFRR261 pKa = 11.84FDD263 pKa = 4.28GMLVEE268 pKa = 4.43PVYY271 pKa = 10.58HH272 pKa = 6.79GKK274 pKa = 9.02HH275 pKa = 6.08AYY277 pKa = 9.77NLLHH281 pKa = 7.3ADD283 pKa = 3.9PNVLTDD289 pKa = 4.03LSKK292 pKa = 10.84AYY294 pKa = 9.24PLLYY298 pKa = 9.84HH299 pKa = 6.97RR300 pKa = 11.84SSNRR304 pKa = 11.84EE305 pKa = 3.16WFRR308 pKa = 11.84NLFLDD313 pKa = 3.44WHH315 pKa = 6.57GEE317 pKa = 4.28VPSLDD322 pKa = 4.0YY323 pKa = 11.15LDD325 pKa = 5.34CIYY328 pKa = 11.17DD329 pKa = 3.59GFEE332 pKa = 3.67

Molecular weight: 38.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

978

332

646

489.0

55.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.419 ± 0.898

2.045 ± 0.142

6.033 ± 0.534

5.624 ± 1.02

4.09 ± 0.256

6.135 ± 1.193

2.352 ± 0.035

5.521 ± 0.239

7.771 ± 0.863

10.941 ± 0.841

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.094

4.294 ± 0.586

4.09 ± 0.795

4.09 ± 0.104

5.01 ± 0.425

6.646 ± 0.528

5.112 ± 1.254

6.851 ± 0.673

2.147 ± 0.696

2.658 ± 0.571
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski