Beihai barnacle virus 3
Average proteome isoelectric point is 5.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJS1|A0A1L3KJS1_9VIRU Uncharacterized protein OS=Beihai barnacle virus 3 OX=1922361 PE=4 SV=1
MM1 pKa = 7.14 FRR3 pKa = 11.84 ASLLQVSPPLYY14 pKa = 8.88 EE15 pKa = 4.0 WPVADD20 pKa = 4.81 AEE22 pKa = 4.59 VRR24 pKa = 11.84 DD25 pKa = 4.1 STASIDD31 pKa = 3.58 SGINQPVLGVKK42 pKa = 10.41 AILGKK47 pKa = 10.41 IKK49 pKa = 10.68 DD50 pKa = 3.88 LTEE53 pKa = 4.81 LIEE56 pKa = 4.49 GEE58 pKa = 4.26 IVDD61 pKa = 4.32 LAVVVDD67 pKa = 4.81 SIEE70 pKa = 4.03 ATTLEE75 pKa = 4.13 IAASVDD81 pKa = 3.82 VIAGEE86 pKa = 3.89 ITEE89 pKa = 4.25 IAIATTDD96 pKa = 3.65 CAVALDD102 pKa = 4.68 SIAVTVDD109 pKa = 2.74 LHH111 pKa = 7.01 NVVPWPCVGII121 pKa = 4.13
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.193
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.897
Patrickios 3.452
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A1L3KJJ2|A0A1L3KJJ2_9VIRU Uncharacterized protein OS=Beihai barnacle virus 3 OX=1922361 PE=4 SV=1
MM1 pKa = 7.52 PRR3 pKa = 11.84 FGRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 FAPRR12 pKa = 11.84 RR13 pKa = 11.84 GRR15 pKa = 11.84 ARR17 pKa = 11.84 GGPARR22 pKa = 11.84 PQNVTVNIKK31 pKa = 10.56 LSQTDD36 pKa = 3.46 ANADD40 pKa = 3.45 GSNPAVYY47 pKa = 10.39 VSLSSQQIIDD57 pKa = 4.06 HH58 pKa = 6.34 GPVLNGIKK66 pKa = 9.89 PAFARR71 pKa = 11.84 IRR73 pKa = 11.84 GMTLEE78 pKa = 4.35 VTSGAAATTEE88 pKa = 4.49 GIGAYY93 pKa = 7.3 MAWRR97 pKa = 11.84 GVMPDD102 pKa = 3.58 TTTMDD107 pKa = 3.68 LGATILEE114 pKa = 4.41 AATRR118 pKa = 11.84 RR119 pKa = 11.84 SAPGISARR127 pKa = 11.84 HH128 pKa = 4.61 TWRR131 pKa = 11.84 NFPTRR136 pKa = 11.84 IRR138 pKa = 11.84 LRR140 pKa = 11.84 TQGAEE145 pKa = 3.82 RR146 pKa = 11.84 TDD148 pKa = 3.12 IQGNVSHH155 pKa = 7.22 HH156 pKa = 6.94 DD157 pKa = 3.53 VFGVGVTYY165 pKa = 10.03 PKK167 pKa = 10.56 GAPPPLAILKK177 pKa = 8.67 ATFQYY182 pKa = 10.9 GGADD186 pKa = 3.17 MVSAEE191 pKa = 4.26 VVEE194 pKa = 4.53 GTGEE198 pKa = 3.58 IEE200 pKa = 4.09 YY201 pKa = 10.48 DD202 pKa = 3.19 HH203 pKa = 7.2 EE204 pKa = 4.46 YY205 pKa = 10.03 PAEE208 pKa = 4.1 KK209 pKa = 9.35 LTRR212 pKa = 11.84 DD213 pKa = 3.84 FLDD216 pKa = 3.38 QANEE220 pKa = 3.92 KK221 pKa = 10.7 LGIPLGYY228 pKa = 9.2 TSDD231 pKa = 3.35 SGAICFVRR239 pKa = 11.84 MLVVYY244 pKa = 10.68 LCDD247 pKa = 3.68 SKK249 pKa = 11.36 EE250 pKa = 3.97 LYY252 pKa = 10.23 AVNSGALTSFKK263 pKa = 10.67 LPYY266 pKa = 9.99 EE267 pKa = 4.24 SFVFPARR274 pKa = 11.84 SSSNEE279 pKa = 3.14 GKK281 pKa = 10.5 LYY283 pKa = 10.82 SRR285 pKa = 11.84 INAWCQTPITPPGAAPGEE303 pKa = 4.62 VYY305 pKa = 9.68 PAHH308 pKa = 7.22 ADD310 pKa = 3.62 DD311 pKa = 4.4 VNLVGDD317 pKa = 4.5 FEE319 pKa = 6.15 HH320 pKa = 7.69 DD321 pKa = 3.73 FGIHH325 pKa = 6.32 DD326 pKa = 3.83 MYY328 pKa = 10.95 VRR330 pKa = 11.84 SVMITPMTRR339 pKa = 11.84 TAGEE343 pKa = 4.52 VLTMAASGAAAVATYY358 pKa = 9.2 PVIPASSSS366 pKa = 3.02
Molecular weight: 39.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.61
IPC2_protein 6.576
IPC_protein 6.839
Toseland 6.927
ProMoST 7.059
Dawson 7.293
Bjellqvist 7.102
Wikipedia 7.278
Rodwell 7.278
Grimsley 7.059
Solomon 7.366
Lehninger 7.38
Nozaki 7.424
DTASelect 7.556
Thurlkill 7.585
EMBOSS 7.658
Sillero 7.658
Patrickios 4.253
IPC_peptide 7.366
IPC2_peptide 7.205
IPC2.peptide.svr19 7.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3376
121
2315
675.2
74.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.893 ± 0.733
1.718 ± 0.217
5.954 ± 0.599
6.783 ± 1.27
3.347 ± 0.251
7.109 ± 0.794
2.844 ± 0.274
4.976 ± 0.634
4.502 ± 0.954
7.05 ± 0.739
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.169 ± 0.534
3.732 ± 0.367
4.769 ± 0.536
3.614 ± 0.312
6.309 ± 0.618
6.309 ± 0.729
5.509 ± 0.581
7.82 ± 0.802
1.422 ± 0.177
3.169 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here