Amino acid dipepetide frequency for Synechococcus phage Bellamy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.305AlaAla: 5.305 ± 0.417
0.625AlaCys: 0.625 ± 0.103
3.872AlaAsp: 3.872 ± 0.317
3.765AlaGlu: 3.765 ± 0.294
2.18AlaPhe: 2.18 ± 0.163
7.362AlaGly: 7.362 ± 0.805
0.976AlaHis: 0.976 ± 0.124
3.765AlaIle: 3.765 ± 0.262
3.872AlaLys: 3.872 ± 0.307
4.192AlaLeu: 4.192 ± 0.298
1.402AlaMet: 1.402 ± 0.173
3.536AlaAsn: 3.536 ± 0.241
2.896AlaPro: 2.896 ± 0.208
2.027AlaGln: 2.027 ± 0.193
2.576AlaArg: 2.576 ± 0.199
4.847AlaSer: 4.847 ± 0.308
5.747AlaThr: 5.747 ± 0.459
4.039AlaVal: 4.039 ± 0.289
0.473AlaTrp: 0.473 ± 0.088
2.347AlaTyr: 2.347 ± 0.189
0.0AlaXaa: 0.0 ± 0.0
Cys
0.747CysAla: 0.747 ± 0.13
0.03CysCys: 0.03 ± 0.019
0.808CysAsp: 0.808 ± 0.141
0.655CysGlu: 0.655 ± 0.122
0.412CysPhe: 0.412 ± 0.084
0.655CysGly: 0.655 ± 0.106
0.259CysHis: 0.259 ± 0.069
0.427CysIle: 0.427 ± 0.096
0.625CysLys: 0.625 ± 0.11
0.64CysLeu: 0.64 ± 0.11
0.244CysMet: 0.244 ± 0.06
0.396CysAsn: 0.396 ± 0.081
0.503CysPro: 0.503 ± 0.1
0.229CysGln: 0.229 ± 0.065
0.442CysArg: 0.442 ± 0.094
0.594CysSer: 0.594 ± 0.115
0.579CysThr: 0.579 ± 0.106
0.518CysVal: 0.518 ± 0.097
0.091CysTrp: 0.091 ± 0.036
0.335CysTyr: 0.335 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
4.085AspAla: 4.085 ± 0.229
0.655AspCys: 0.655 ± 0.119
4.756AspAsp: 4.756 ± 0.335
3.811AspGlu: 3.811 ± 0.29
3.171AspPhe: 3.171 ± 0.229
5.625AspGly: 5.625 ± 0.39
0.899AspHis: 0.899 ± 0.139
4.436AspIle: 4.436 ± 0.279
3.735AspLys: 3.735 ± 0.306
5.076AspLeu: 5.076 ± 0.324
1.479AspMet: 1.479 ± 0.169
3.384AspAsn: 3.384 ± 0.266
2.774AspPro: 2.774 ± 0.232
2.073AspGln: 2.073 ± 0.177
2.424AspArg: 2.424 ± 0.216
3.963AspSer: 3.963 ± 0.269
3.918AspThr: 3.918 ± 0.266
4.466AspVal: 4.466 ± 0.276
0.93AspTrp: 0.93 ± 0.147
3.293AspTyr: 3.293 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
3.308GluAla: 3.308 ± 0.291
0.884GluCys: 0.884 ± 0.142
4.161GluAsp: 4.161 ± 0.284
5.122GluGlu: 5.122 ± 0.528
3.094GluPhe: 3.094 ± 0.23
4.146GluGly: 4.146 ± 0.234
1.098GluHis: 1.098 ± 0.141
4.71GluIle: 4.71 ± 0.31
4.222GluLys: 4.222 ± 0.355
4.802GluLeu: 4.802 ± 0.323
1.433GluMet: 1.433 ± 0.187
3.262GluAsn: 3.262 ± 0.254
1.875GluPro: 1.875 ± 0.169
2.226GluGln: 2.226 ± 0.234
2.165GluArg: 2.165 ± 0.169
3.674GluSer: 3.674 ± 0.246
3.933GluThr: 3.933 ± 0.231
4.542GluVal: 4.542 ± 0.301
0.762GluTrp: 0.762 ± 0.12
2.744GluTyr: 2.744 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
2.744PheAla: 2.744 ± 0.187
0.701PheCys: 0.701 ± 0.117
3.628PheAsp: 3.628 ± 0.235
2.927PheGlu: 2.927 ± 0.229
2.012PhePhe: 2.012 ± 0.181
3.186PheGly: 3.186 ± 0.237
0.655PheHis: 0.655 ± 0.106
2.378PheIle: 2.378 ± 0.195
2.835PheLys: 2.835 ± 0.26
3.247PheLeu: 3.247 ± 0.228
0.899PheMet: 0.899 ± 0.136
2.561PheAsn: 2.561 ± 0.221
1.722PhePro: 1.722 ± 0.204
1.372PheGln: 1.372 ± 0.158
1.448PheArg: 1.448 ± 0.21
3.003PheSer: 3.003 ± 0.21
2.881PheThr: 2.881 ± 0.25
3.445PheVal: 3.445 ± 0.247
0.488PheTrp: 0.488 ± 0.089
1.616PheTyr: 1.616 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
6.677GlyAla: 6.677 ± 0.69
0.747GlyCys: 0.747 ± 0.118
4.893GlyAsp: 4.893 ± 0.279
3.978GlyGlu: 3.978 ± 0.266
3.079GlyPhe: 3.079 ± 0.22
15.441GlyGly: 15.441 ± 3.41
1.265GlyHis: 1.265 ± 0.154
6.463GlyIle: 6.463 ± 0.547
4.405GlyLys: 4.405 ± 0.333
5.213GlyLeu: 5.213 ± 0.326
1.997GlyMet: 1.997 ± 0.214
4.695GlyAsn: 4.695 ± 0.341
2.637GlyPro: 2.637 ± 0.375
2.104GlyGln: 2.104 ± 0.184
3.064GlyArg: 3.064 ± 0.317
7.195GlySer: 7.195 ± 0.734
7.286GlyThr: 7.286 ± 0.623
6.158GlyVal: 6.158 ± 0.475
1.067GlyTrp: 1.067 ± 0.138
3.201GlyTyr: 3.201 ± 0.234
0.0GlyXaa: 0.0 ± 0.0
His
0.777HisAla: 0.777 ± 0.109
0.183HisCys: 0.183 ± 0.056
0.976HisAsp: 0.976 ± 0.105
0.93HisGlu: 0.93 ± 0.158
1.067HisPhe: 1.067 ± 0.14
1.219HisGly: 1.219 ± 0.14
0.518HisHis: 0.518 ± 0.107
1.021HisIle: 1.021 ± 0.135
1.418HisLys: 1.418 ± 0.171
1.113HisLeu: 1.113 ± 0.174
0.381HisMet: 0.381 ± 0.084
0.838HisAsn: 0.838 ± 0.127
1.082HisPro: 1.082 ± 0.127
0.518HisGln: 0.518 ± 0.105
0.747HisArg: 0.747 ± 0.101
0.793HisSer: 0.793 ± 0.102
1.128HisThr: 1.128 ± 0.113
0.93HisVal: 0.93 ± 0.117
0.198HisTrp: 0.198 ± 0.058
0.762HisTyr: 0.762 ± 0.118
0.0HisXaa: 0.0 ± 0.0
Ile
3.811IleAla: 3.811 ± 0.226
0.747IleCys: 0.747 ± 0.126
4.375IleAsp: 4.375 ± 0.287
4.39IleGlu: 4.39 ± 0.263
2.363IlePhe: 2.363 ± 0.194
5.198IleGly: 5.198 ± 0.442
1.021IleHis: 1.021 ± 0.129
3.445IleIle: 3.445 ± 0.277
4.405IleLys: 4.405 ± 0.301
4.131IleLeu: 4.131 ± 0.253
1.052IleMet: 1.052 ± 0.147
3.567IleAsn: 3.567 ± 0.32
2.957IlePro: 2.957 ± 0.232
2.18IleGln: 2.18 ± 0.216
2.805IleArg: 2.805 ± 0.238
4.649IleSer: 4.649 ± 0.323
5.137IleThr: 5.137 ± 0.408
4.1IleVal: 4.1 ± 0.325
0.412IleTrp: 0.412 ± 0.079
2.012IleTyr: 2.012 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
3.658LysAla: 3.658 ± 0.295
0.412LysCys: 0.412 ± 0.085
3.704LysAsp: 3.704 ± 0.303
4.558LysGlu: 4.558 ± 0.434
2.896LysPhe: 2.896 ± 0.273
3.491LysGly: 3.491 ± 0.298
0.976LysHis: 0.976 ± 0.156
4.314LysIle: 4.314 ± 0.313
6.189LysLys: 6.189 ± 0.612
5.0LysLeu: 5.0 ± 0.335
1.722LysMet: 1.722 ± 0.251
3.232LysAsn: 3.232 ± 0.24
2.622LysPro: 2.622 ± 0.266
2.104LysGln: 2.104 ± 0.191
2.576LysArg: 2.576 ± 0.27
3.948LysSer: 3.948 ± 0.294
3.841LysThr: 3.841 ± 0.254
4.527LysVal: 4.527 ± 0.4
0.732LysTrp: 0.732 ± 0.118
2.668LysTyr: 2.668 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
4.588LeuAla: 4.588 ± 0.323
0.549LeuCys: 0.549 ± 0.103
5.259LeuAsp: 5.259 ± 0.321
4.71LeuGlu: 4.71 ± 0.263
2.805LeuPhe: 2.805 ± 0.21
4.969LeuGly: 4.969 ± 0.316
1.448LeuHis: 1.448 ± 0.154
3.582LeuIle: 3.582 ± 0.237
5.015LeuLys: 5.015 ± 0.325
4.756LeuLeu: 4.756 ± 0.312
1.479LeuMet: 1.479 ± 0.175
4.497LeuAsn: 4.497 ± 0.285
3.232LeuPro: 3.232 ± 0.235
2.591LeuGln: 2.591 ± 0.199
2.988LeuArg: 2.988 ± 0.206
4.451LeuSer: 4.451 ± 0.274
5.183LeuThr: 5.183 ± 0.328
3.887LeuVal: 3.887 ± 0.286
0.625LeuTrp: 0.625 ± 0.115
3.049LeuTyr: 3.049 ± 0.241
0.0LeuXaa: 0.0 ± 0.0
Met
1.509MetAla: 1.509 ± 0.163
0.335MetCys: 0.335 ± 0.079
1.235MetAsp: 1.235 ± 0.182
1.601MetGlu: 1.601 ± 0.166
0.976MetPhe: 0.976 ± 0.124
1.25MetGly: 1.25 ± 0.149
0.305MetHis: 0.305 ± 0.083
1.311MetIle: 1.311 ± 0.152
2.119MetLys: 2.119 ± 0.23
1.57MetLeu: 1.57 ± 0.186
0.625MetMet: 0.625 ± 0.123
1.25MetAsn: 1.25 ± 0.138
0.899MetPro: 0.899 ± 0.134
0.625MetGln: 0.625 ± 0.101
1.067MetArg: 1.067 ± 0.152
1.601MetSer: 1.601 ± 0.174
1.402MetThr: 1.402 ± 0.179
1.143MetVal: 1.143 ± 0.147
0.168MetTrp: 0.168 ± 0.058
0.854MetTyr: 0.854 ± 0.108
0.0MetXaa: 0.0 ± 0.0
Asn
3.338AsnAla: 3.338 ± 0.237
0.442AsnCys: 0.442 ± 0.081
3.232AsnAsp: 3.232 ± 0.236
2.561AsnGlu: 2.561 ± 0.176
3.003AsnPhe: 3.003 ± 0.199
4.649AsnGly: 4.649 ± 0.332
1.128AsnHis: 1.128 ± 0.107
3.826AsnIle: 3.826 ± 0.276
3.094AsnLys: 3.094 ± 0.228
4.588AsnLeu: 4.588 ± 0.292
0.915AsnMet: 0.915 ± 0.121
2.866AsnAsn: 2.866 ± 0.218
3.354AsnPro: 3.354 ± 0.252
1.875AsnGln: 1.875 ± 0.19
2.378AsnArg: 2.378 ± 0.196
3.43AsnSer: 3.43 ± 0.284
3.811AsnThr: 3.811 ± 0.403
4.314AsnVal: 4.314 ± 0.335
0.564AsnTrp: 0.564 ± 0.094
1.799AsnTyr: 1.799 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
2.607ProAla: 2.607 ± 0.228
0.29ProCys: 0.29 ± 0.063
2.957ProAsp: 2.957 ± 0.238
2.911ProGlu: 2.911 ± 0.253
1.646ProPhe: 1.646 ± 0.159
4.222ProGly: 4.222 ± 0.47
0.899ProHis: 0.899 ± 0.122
2.149ProIle: 2.149 ± 0.229
2.622ProLys: 2.622 ± 0.21
2.652ProLeu: 2.652 ± 0.178
0.716ProMet: 0.716 ± 0.116
2.134ProAsn: 2.134 ± 0.201
1.631ProPro: 1.631 ± 0.254
1.418ProGln: 1.418 ± 0.14
1.585ProArg: 1.585 ± 0.178
2.744ProSer: 2.744 ± 0.223
2.896ProThr: 2.896 ± 0.208
3.049ProVal: 3.049 ± 0.188
0.488ProTrp: 0.488 ± 0.087
1.783ProTyr: 1.783 ± 0.188
0.0ProXaa: 0.0 ± 0.0
Gln
1.692GlnAla: 1.692 ± 0.157
0.107GlnCys: 0.107 ± 0.044
1.844GlnAsp: 1.844 ± 0.162
2.378GlnGlu: 2.378 ± 0.221
1.524GlnPhe: 1.524 ± 0.168
2.439GlnGly: 2.439 ± 0.209
0.457GlnHis: 0.457 ± 0.084
2.378GlnIle: 2.378 ± 0.192
1.966GlnLys: 1.966 ± 0.236
2.408GlnLeu: 2.408 ± 0.209
1.037GlnMet: 1.037 ± 0.149
2.073GlnAsn: 2.073 ± 0.164
1.296GlnPro: 1.296 ± 0.162
1.372GlnGln: 1.372 ± 0.156
1.524GlnArg: 1.524 ± 0.144
2.149GlnSer: 2.149 ± 0.197
1.951GlnThr: 1.951 ± 0.172
2.21GlnVal: 2.21 ± 0.186
0.213GlnTrp: 0.213 ± 0.055
1.646GlnTyr: 1.646 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
2.576ArgAla: 2.576 ± 0.244
0.381ArgCys: 0.381 ± 0.091
2.347ArgAsp: 2.347 ± 0.227
2.668ArgGlu: 2.668 ± 0.204
2.058ArgPhe: 2.058 ± 0.227
2.957ArgGly: 2.957 ± 0.223
0.64ArgHis: 0.64 ± 0.104
2.744ArgIle: 2.744 ± 0.261
2.866ArgLys: 2.866 ± 0.266
2.561ArgLeu: 2.561 ± 0.192
1.357ArgMet: 1.357 ± 0.161
2.18ArgAsn: 2.18 ± 0.172
1.28ArgPro: 1.28 ± 0.125
1.448ArgGln: 1.448 ± 0.168
2.165ArgArg: 2.165 ± 0.223
2.5ArgSer: 2.5 ± 0.187
2.165ArgThr: 2.165 ± 0.21
2.652ArgVal: 2.652 ± 0.339
0.473ArgTrp: 0.473 ± 0.091
2.012ArgTyr: 2.012 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
5.061SerAla: 5.061 ± 0.317
0.473SerCys: 0.473 ± 0.1
4.329SerAsp: 4.329 ± 0.308
3.597SerGlu: 3.597 ± 0.227
3.079SerPhe: 3.079 ± 0.236
7.53SerGly: 7.53 ± 0.572
0.945SerHis: 0.945 ± 0.129
3.918SerIle: 3.918 ± 0.262
3.155SerLys: 3.155 ± 0.244
4.924SerLeu: 4.924 ± 0.317
1.54SerMet: 1.54 ± 0.176
4.024SerAsn: 4.024 ± 0.291
2.637SerPro: 2.637 ± 0.289
1.936SerGln: 1.936 ± 0.196
2.469SerArg: 2.469 ± 0.17
4.908SerSer: 4.908 ± 0.413
5.564SerThr: 5.564 ± 0.546
5.03SerVal: 5.03 ± 0.453
0.579SerTrp: 0.579 ± 0.092
2.607SerTyr: 2.607 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
5.655ThrAla: 5.655 ± 0.465
0.335ThrCys: 0.335 ± 0.08
3.658ThrAsp: 3.658 ± 0.231
3.918ThrGlu: 3.918 ± 0.228
3.46ThrPhe: 3.46 ± 0.308
7.378ThrGly: 7.378 ± 0.612
0.945ThrHis: 0.945 ± 0.11
4.68ThrIle: 4.68 ± 0.32
3.567ThrLys: 3.567 ± 0.249
5.228ThrLeu: 5.228 ± 0.361
1.082ThrMet: 1.082 ± 0.123
3.643ThrAsn: 3.643 ± 0.295
3.399ThrPro: 3.399 ± 0.217
2.347ThrGln: 2.347 ± 0.168
2.515ThrArg: 2.515 ± 0.209
5.228ThrSer: 5.228 ± 0.608
5.869ThrThr: 5.869 ± 0.539
5.914ThrVal: 5.914 ± 0.405
0.442ThrTrp: 0.442 ± 0.08
2.515ThrTyr: 2.515 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
4.527ValAla: 4.527 ± 0.292
0.671ValCys: 0.671 ± 0.098
4.847ValAsp: 4.847 ± 0.307
4.268ValGlu: 4.268 ± 0.272
2.668ValPhe: 2.668 ± 0.193
6.692ValGly: 6.692 ± 0.777
1.052ValHis: 1.052 ± 0.143
4.009ValIle: 4.009 ± 0.228
3.963ValLys: 3.963 ± 0.279
4.116ValLeu: 4.116 ± 0.258
1.479ValMet: 1.479 ± 0.152
4.421ValAsn: 4.421 ± 0.325
2.637ValPro: 2.637 ± 0.189
2.165ValGln: 2.165 ± 0.166
2.683ValArg: 2.683 ± 0.21
5.472ValSer: 5.472 ± 0.355
5.609ValThr: 5.609 ± 0.467
5.061ValVal: 5.061 ± 0.292
0.564ValTrp: 0.564 ± 0.098
2.393ValTyr: 2.393 ± 0.18
0.0ValXaa: 0.0 ± 0.0
Trp
0.564TrpAla: 0.564 ± 0.089
0.137TrpCys: 0.137 ± 0.044
0.732TrpAsp: 0.732 ± 0.121
0.747TrpGlu: 0.747 ± 0.12
0.503TrpPhe: 0.503 ± 0.111
0.716TrpGly: 0.716 ± 0.102
0.29TrpHis: 0.29 ± 0.068
0.579TrpIle: 0.579 ± 0.094
0.686TrpLys: 0.686 ± 0.123
0.777TrpLeu: 0.777 ± 0.128
0.213TrpMet: 0.213 ± 0.052
0.442TrpAsn: 0.442 ± 0.102
0.274TrpPro: 0.274 ± 0.064
0.457TrpGln: 0.457 ± 0.076
0.549TrpArg: 0.549 ± 0.106
0.564TrpSer: 0.564 ± 0.105
0.549TrpThr: 0.549 ± 0.103
0.594TrpVal: 0.594 ± 0.111
0.213TrpTrp: 0.213 ± 0.067
0.396TrpTyr: 0.396 ± 0.068
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.576TyrAla: 2.576 ± 0.187
0.488TyrCys: 0.488 ± 0.087
3.338TyrAsp: 3.338 ± 0.199
2.668TyrGlu: 2.668 ± 0.204
1.753TyrPhe: 1.753 ± 0.155
2.439TyrGly: 2.439 ± 0.193
0.823TyrHis: 0.823 ± 0.108
2.622TyrIle: 2.622 ± 0.256
2.424TyrLys: 2.424 ± 0.225
2.668TyrLeu: 2.668 ± 0.204
0.777TyrMet: 0.777 ± 0.108
2.165TyrAsn: 2.165 ± 0.172
1.692TyrPro: 1.692 ± 0.169
1.601TyrGln: 1.601 ± 0.177
1.86TyrArg: 1.86 ± 0.17
2.591TyrSer: 2.591 ± 0.213
2.332TyrThr: 2.332 ± 0.18
2.713TyrVal: 2.713 ± 0.214
0.503TyrTrp: 0.503 ± 0.103
1.89TyrTyr: 1.89 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 269 proteins (65604 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski