Marinomonas phage YY
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1GTT5|A0A2S1GTT5_9VIRU Uncharacterized protein OS=Marinomonas phage YY OX=2163588 PE=4 SV=1
MM1 pKa = 6.5 TTEE4 pKa = 4.69 FLPALEE10 pKa = 4.26 SVVLNSPPAFEE21 pKa = 5.71 AEE23 pKa = 4.29 LPPMLMEE30 pKa = 5.24 PDD32 pKa = 4.34 AFAQYY37 pKa = 10.84 EE38 pKa = 4.05 PDD40 pKa = 3.47 AANAGHH46 pKa = 6.93 APDD49 pKa = 4.07 MAPAAEE55 pKa = 4.64 VIDD58 pKa = 5.06 LLAEE62 pKa = 4.42 PDD64 pKa = 4.96 FLEE67 pKa = 4.43 QWALMHH73 pKa = 7.04 DD74 pKa = 3.91 MAGGMIQMRR83 pKa = 11.84 TGAPCPLGDD92 pKa = 3.69 QARR95 pKa = 11.84 SAGGMAAGKK104 pKa = 9.59 AAYY107 pKa = 9.63 QLISSNPALAKK118 pKa = 10.55 LILGTEE124 pKa = 4.26 STFLGQLAAIGMHH137 pKa = 6.59 GFACVQVVKK146 pKa = 10.6 ASARR150 pKa = 11.84 GDD152 pKa = 3.48 TLPQIEE158 pKa = 4.73 PEE160 pKa = 4.07 KK161 pKa = 10.61 EE162 pKa = 3.72 AAA164 pKa = 3.88
Molecular weight: 17.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.31
IPC2_protein 4.253
IPC_protein 4.164
Toseland 3.999
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 3.986
Rodwell 3.999
Grimsley 3.91
Solomon 4.101
Lehninger 4.062
Nozaki 4.228
DTASelect 4.355
Thurlkill 4.012
EMBOSS 3.999
Sillero 4.279
Patrickios 3.541
IPC_peptide 4.113
IPC2_peptide 4.266
IPC2.peptide.svr19 4.193
Protein with the highest isoelectric point:
>tr|A0A2S1GTR1|A0A2S1GTR1_9VIRU DNA helicase OS=Marinomonas phage YY OX=2163588 PE=4 SV=1
MM1 pKa = 7.53 TSPTFKK7 pKa = 10.71 TEE9 pKa = 3.25 ITAVWGCRR17 pKa = 11.84 GSGKK21 pKa = 7.28 TTLAKK26 pKa = 10.57 KK27 pKa = 10.1 LVADD31 pKa = 4.03 HH32 pKa = 7.16 RR33 pKa = 11.84 PAQVLFIDD41 pKa = 4.19 PLAAEE46 pKa = 4.67 GVDD49 pKa = 3.67 VFGVAPAIRR58 pKa = 11.84 DD59 pKa = 3.67 GQKK62 pKa = 10.78 LVVCNAQSKK71 pKa = 9.25 DD72 pKa = 3.6 HH73 pKa = 6.62 QIGALLTAYY82 pKa = 10.2 ALSTPTRR89 pKa = 11.84 PVYY92 pKa = 10.26 AICDD96 pKa = 3.61 EE97 pKa = 4.35 APAYY101 pKa = 10.6 LDD103 pKa = 3.61 RR104 pKa = 11.84 SSAALNKK111 pKa = 10.04 IMFQGRR117 pKa = 11.84 HH118 pKa = 5.6 AGFGMCIIGQRR129 pKa = 11.84 PAAVDD134 pKa = 3.39 AQIRR138 pKa = 11.84 SQAAVTYY145 pKa = 8.15 WLKK148 pKa = 10.82 LADD151 pKa = 3.91 HH152 pKa = 6.74 VDD154 pKa = 3.25 VGVAAKK160 pKa = 10.63 SLGTEE165 pKa = 4.28 RR166 pKa = 11.84 ARR168 pKa = 11.84 SLQTLAPGEE177 pKa = 4.37 FIKK180 pKa = 10.93 HH181 pKa = 5.85 PEE183 pKa = 3.63
Molecular weight: 19.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.983
IPC2_protein 8.053
IPC_protein 8.053
Toseland 8.068
ProMoST 8.463
Dawson 8.726
Bjellqvist 8.946
Wikipedia 8.682
Rodwell 8.77
Grimsley 7.834
Solomon 8.96
Lehninger 8.96
Nozaki 9.194
DTASelect 8.712
Thurlkill 8.799
EMBOSS 8.946
Sillero 9.092
Patrickios 4.711
IPC_peptide 8.946
IPC2_peptide 7.878
IPC2.peptide.svr19 7.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
584
78
183
146.0
15.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.726 ± 1.687
1.712 ± 0.401
5.822 ± 0.418
5.651 ± 0.99
3.938 ± 0.385
7.877 ± 0.119
2.74 ± 0.397
5.479 ± 0.678
3.253 ± 0.947
8.219 ± 0.969
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.253 ± 1.036
2.397 ± 0.603
7.192 ± 0.813
4.623 ± 0.353
6.507 ± 1.887
4.623 ± 0.213
4.281 ± 0.887
4.623 ± 1.075
1.541 ± 0.625
1.541 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here