Xanthomonas phage KPhi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Naesvirus; unclassified Naesvirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1GLE3|A0A3G1GLE3_9CAUD Terminase-like family protein OS=Xanthomonas phage KPhi1 OX=1927017 GN=K1pha_27 PE=4 SV=1
MM1 pKa = 7.63PAATLLPTEE10 pKa = 4.69DD11 pKa = 3.14QTFQALARR19 pKa = 11.84WVGKK23 pKa = 10.56VLDD26 pKa = 4.63LPSNTLQIVKK36 pKa = 10.03GFQNLTATPTGSYY49 pKa = 9.75VVISPGTMQRR59 pKa = 11.84QDD61 pKa = 2.99FGRR64 pKa = 11.84RR65 pKa = 11.84YY66 pKa = 9.63YY67 pKa = 11.15DD68 pKa = 3.73PDD70 pKa = 3.19NSQAVQQAHH79 pKa = 5.79LTYY82 pKa = 10.24SYY84 pKa = 11.12QVDD87 pKa = 4.12CYY89 pKa = 11.03GPNGPTWASVLSVAWRR105 pKa = 11.84SMWGVDD111 pKa = 3.84NMAPPILTPLYY122 pKa = 10.84ADD124 pKa = 5.1APQQLNIVNAQGQYY138 pKa = 6.88EE139 pKa = 4.22QRR141 pKa = 11.84FMIRR145 pKa = 11.84LFGQVNQDD153 pKa = 2.63VGLPQDD159 pKa = 3.71FFEE162 pKa = 5.23QIQLDD167 pKa = 4.05SFIVADD173 pKa = 3.82QLPP176 pKa = 3.6

Molecular weight: 19.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1GLF1|A0A3G1GLF1_9CAUD Uncharacterized protein OS=Xanthomonas phage KPhi1 OX=1927017 GN=K1pha_37 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.19SGSTATVNEE11 pKa = 4.19LSRR14 pKa = 11.84RR15 pKa = 11.84QAAQYY20 pKa = 11.19AFTASLEE27 pKa = 4.13KK28 pKa = 10.6HH29 pKa = 5.71RR30 pKa = 11.84ATLARR35 pKa = 11.84ALQADD40 pKa = 4.68RR41 pKa = 11.84LWLRR45 pKa = 11.84TRR47 pKa = 11.84RR48 pKa = 11.84LCACWCASLAPVRR61 pKa = 11.84PTARR65 pKa = 11.84RR66 pKa = 11.84CASSKK71 pKa = 10.14RR72 pKa = 11.84LSDD75 pKa = 3.18RR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 3.63YY79 pKa = 11.28SVFVMLWRR87 pKa = 11.84KK88 pKa = 7.61EE89 pKa = 3.98MKK91 pKa = 9.93IKK93 pKa = 10.5EE94 pKa = 3.75IAAALGVTPRR104 pKa = 11.84MVNNHH109 pKa = 5.43KK110 pKa = 10.55RR111 pKa = 11.84SIKK114 pKa = 10.79AKK116 pKa = 8.25MKK118 pKa = 10.79KK119 pKa = 9.39EE120 pKa = 3.97FNVV123 pKa = 3.28

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13892

36

827

210.5

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.13 ± 0.557

1.015 ± 0.124

5.903 ± 0.275

4.794 ± 0.266

3.124 ± 0.167

7.861 ± 0.609

1.778 ± 0.157

4.319 ± 0.2

3.225 ± 0.222

8.523 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.161

3.34 ± 0.198

5.543 ± 0.321

4.636 ± 0.279

6.601 ± 0.325

5.874 ± 0.25

6.407 ± 0.31

7.119 ± 0.258

1.749 ± 0.13

2.635 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski