Amino acid dipepetide frequency for Streptomyces phage Darolandstone

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
27.19AlaAla: 27.19 ± 1.764
1.007AlaCys: 1.007 ± 0.351
9.528AlaAsp: 9.528 ± 1.082
11.93AlaGlu: 11.93 ± 1.364
2.092AlaPhe: 2.092 ± 0.418
12.472AlaGly: 12.472 ± 1.2
2.634AlaHis: 2.634 ± 0.49
3.796AlaIle: 3.796 ± 0.514
3.563AlaLys: 3.563 ± 0.762
13.556AlaLeu: 13.556 ± 1.731
3.641AlaMet: 3.641 ± 0.557
2.401AlaAsn: 2.401 ± 0.458
9.063AlaPro: 9.063 ± 0.849
6.197AlaGln: 6.197 ± 0.624
12.317AlaArg: 12.317 ± 1.157
4.958AlaSer: 4.958 ± 0.569
8.289AlaThr: 8.289 ± 0.849
11.078AlaVal: 11.078 ± 1.092
3.099AlaTrp: 3.099 ± 0.456
3.331AlaTyr: 3.331 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
0.465CysAla: 0.465 ± 0.221
0.077CysCys: 0.077 ± 0.076
0.62CysAsp: 0.62 ± 0.392
0.387CysGlu: 0.387 ± 0.198
0.155CysPhe: 0.155 ± 0.127
0.93CysGly: 0.93 ± 0.294
0.155CysHis: 0.155 ± 0.104
0.0CysIle: 0.0 ± 0.0
0.155CysLys: 0.155 ± 0.106
0.387CysLeu: 0.387 ± 0.178
0.077CysMet: 0.077 ± 0.08
0.31CysAsn: 0.31 ± 0.126
1.239CysPro: 1.239 ± 0.351
0.31CysGln: 0.31 ± 0.2
0.697CysArg: 0.697 ± 0.294
0.31CysSer: 0.31 ± 0.148
0.542CysThr: 0.542 ± 0.206
0.31CysVal: 0.31 ± 0.158
0.077CysTrp: 0.077 ± 0.088
0.077CysTyr: 0.077 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
10.148AspAla: 10.148 ± 1.023
0.775AspCys: 0.775 ± 0.295
5.5AspAsp: 5.5 ± 1.061
3.718AspGlu: 3.718 ± 0.606
0.775AspPhe: 0.775 ± 0.26
7.901AspGly: 7.901 ± 0.836
2.324AspHis: 2.324 ± 0.469
2.324AspIle: 2.324 ± 0.407
0.852AspLys: 0.852 ± 0.235
6.275AspLeu: 6.275 ± 1.11
1.007AspMet: 1.007 ± 0.286
1.162AspAsn: 1.162 ± 0.269
4.725AspPro: 4.725 ± 0.65
3.099AspGln: 3.099 ± 0.469
6.352AspArg: 6.352 ± 0.829
3.021AspSer: 3.021 ± 0.55
3.331AspThr: 3.331 ± 0.54
3.641AspVal: 3.641 ± 0.708
1.007AspTrp: 1.007 ± 0.281
1.162AspTyr: 1.162 ± 0.305
0.0AspXaa: 0.0 ± 0.0
Glu
6.585GluAla: 6.585 ± 0.9
0.31GluCys: 0.31 ± 0.138
3.021GluAsp: 3.021 ± 0.662
2.789GluGlu: 2.789 ± 0.481
1.549GluPhe: 1.549 ± 0.343
4.803GluGly: 4.803 ± 0.711
2.014GluHis: 2.014 ± 0.402
1.162GluIle: 1.162 ± 0.29
0.697GluLys: 0.697 ± 0.27
3.486GluLeu: 3.486 ± 0.636
1.007GluMet: 1.007 ± 0.408
1.085GluAsn: 1.085 ± 0.294
4.416GluPro: 4.416 ± 0.75
6.43GluGln: 6.43 ± 0.898
6.352GluArg: 6.352 ± 1.105
2.711GluSer: 2.711 ± 0.458
2.556GluThr: 2.556 ± 0.431
4.88GluVal: 4.88 ± 0.609
1.239GluTrp: 1.239 ± 0.348
1.394GluTyr: 1.394 ± 0.431
0.0GluXaa: 0.0 ± 0.0
Phe
2.789PheAla: 2.789 ± 0.496
0.155PheCys: 0.155 ± 0.096
1.859PheAsp: 1.859 ± 0.356
1.162PheGlu: 1.162 ± 0.297
0.465PhePhe: 0.465 ± 0.203
2.246PheGly: 2.246 ± 0.422
0.31PheHis: 0.31 ± 0.179
0.62PheIle: 0.62 ± 0.225
0.387PheLys: 0.387 ± 0.155
1.007PheLeu: 1.007 ± 0.267
0.387PheMet: 0.387 ± 0.152
0.62PheAsn: 0.62 ± 0.21
0.852PhePro: 0.852 ± 0.206
0.62PheGln: 0.62 ± 0.185
1.239PheArg: 1.239 ± 0.288
0.775PheSer: 0.775 ± 0.236
1.627PheThr: 1.627 ± 0.302
1.704PheVal: 1.704 ± 0.318
0.387PheTrp: 0.387 ± 0.221
0.077PheTyr: 0.077 ± 0.074
0.0PheXaa: 0.0 ± 0.0
Gly
11.0GlyAla: 11.0 ± 1.545
0.31GlyCys: 0.31 ± 0.14
6.352GlyAsp: 6.352 ± 0.968
6.042GlyGlu: 6.042 ± 0.807
1.937GlyPhe: 1.937 ± 0.352
6.43GlyGly: 6.43 ± 1.054
1.394GlyHis: 1.394 ± 0.338
3.873GlyIle: 3.873 ± 0.617
2.634GlyLys: 2.634 ± 0.47
8.289GlyLeu: 8.289 ± 1.136
1.394GlyMet: 1.394 ± 0.236
1.317GlyAsn: 1.317 ± 0.279
5.345GlyPro: 5.345 ± 0.632
4.183GlyGln: 4.183 ± 0.691
8.831GlyArg: 8.831 ± 1.157
3.099GlySer: 3.099 ± 0.472
7.359GlyThr: 7.359 ± 1.085
5.268GlyVal: 5.268 ± 0.785
1.394GlyTrp: 1.394 ± 0.375
2.556GlyTyr: 2.556 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
1.627HisAla: 1.627 ± 0.331
0.31HisCys: 0.31 ± 0.159
2.014HisAsp: 2.014 ± 0.487
1.317HisGlu: 1.317 ± 0.319
0.387HisPhe: 0.387 ± 0.199
1.859HisGly: 1.859 ± 0.428
0.62HisHis: 0.62 ± 0.2
0.465HisIle: 0.465 ± 0.191
0.465HisLys: 0.465 ± 0.188
1.085HisLeu: 1.085 ± 0.312
0.232HisMet: 0.232 ± 0.138
0.077HisAsn: 0.077 ± 0.071
1.937HisPro: 1.937 ± 0.416
0.387HisGln: 0.387 ± 0.237
2.324HisArg: 2.324 ± 0.473
0.93HisSer: 0.93 ± 0.251
1.394HisThr: 1.394 ± 0.32
2.401HisVal: 2.401 ± 0.583
0.62HisTrp: 0.62 ± 0.205
0.542HisTyr: 0.542 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
4.338IleAla: 4.338 ± 0.494
0.077IleCys: 0.077 ± 0.087
2.014IleAsp: 2.014 ± 0.363
2.944IleGlu: 2.944 ± 0.595
0.31IlePhe: 0.31 ± 0.144
3.641IleGly: 3.641 ± 1.089
0.232IleHis: 0.232 ± 0.138
1.085IleIle: 1.085 ± 0.304
0.93IleLys: 0.93 ± 0.232
2.246IleLeu: 2.246 ± 0.444
0.387IleMet: 0.387 ± 0.193
0.542IleAsn: 0.542 ± 0.274
2.169IlePro: 2.169 ± 0.392
0.465IleGln: 0.465 ± 0.194
2.634IleArg: 2.634 ± 0.405
0.852IleSer: 0.852 ± 0.263
2.634IleThr: 2.634 ± 0.503
2.866IleVal: 2.866 ± 0.452
0.232IleTrp: 0.232 ± 0.135
0.62IleTyr: 0.62 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
4.416LysAla: 4.416 ± 0.627
0.077LysCys: 0.077 ± 0.079
0.62LysAsp: 0.62 ± 0.231
0.697LysGlu: 0.697 ± 0.294
0.232LysPhe: 0.232 ± 0.142
2.479LysGly: 2.479 ± 0.621
0.697LysHis: 0.697 ± 0.181
0.775LysIle: 0.775 ± 0.298
0.465LysLys: 0.465 ± 0.219
2.092LysLeu: 2.092 ± 0.44
0.232LysMet: 0.232 ± 0.11
0.62LysAsn: 0.62 ± 0.19
1.239LysPro: 1.239 ± 0.328
0.155LysGln: 0.155 ± 0.1
2.246LysArg: 2.246 ± 0.418
1.394LysSer: 1.394 ± 0.337
1.317LysThr: 1.317 ± 0.34
1.782LysVal: 1.782 ± 0.372
0.077LysTrp: 0.077 ± 0.094
0.542LysTyr: 0.542 ± 0.181
0.0LysXaa: 0.0 ± 0.0
Leu
17.197LeuAla: 17.197 ± 1.954
0.62LeuCys: 0.62 ± 0.294
8.909LeuAsp: 8.909 ± 0.968
2.014LeuGlu: 2.014 ± 0.443
1.782LeuPhe: 1.782 ± 0.364
7.437LeuGly: 7.437 ± 0.976
1.317LeuHis: 1.317 ± 0.278
2.246LeuIle: 2.246 ± 0.599
2.014LeuLys: 2.014 ± 0.373
9.218LeuLeu: 9.218 ± 0.886
0.697LeuMet: 0.697 ± 0.242
1.549LeuAsn: 1.549 ± 0.307
6.739LeuPro: 6.739 ± 0.726
1.627LeuGln: 1.627 ± 0.385
5.81LeuArg: 5.81 ± 0.601
3.641LeuSer: 3.641 ± 0.548
5.423LeuThr: 5.423 ± 0.675
4.958LeuVal: 4.958 ± 0.894
1.472LeuTrp: 1.472 ± 0.351
2.092LeuTyr: 2.092 ± 0.404
0.0LeuXaa: 0.0 ± 0.0
Met
2.324MetAla: 2.324 ± 0.325
0.077MetCys: 0.077 ± 0.062
0.852MetAsp: 0.852 ± 0.254
0.31MetGlu: 0.31 ± 0.188
0.542MetPhe: 0.542 ± 0.215
1.239MetGly: 1.239 ± 0.332
0.232MetHis: 0.232 ± 0.146
0.62MetIle: 0.62 ± 0.217
0.155MetLys: 0.155 ± 0.108
0.775MetLeu: 0.775 ± 0.253
0.31MetMet: 0.31 ± 0.151
1.085MetAsn: 1.085 ± 0.298
1.239MetPro: 1.239 ± 0.302
0.775MetGln: 0.775 ± 0.199
1.782MetArg: 1.782 ± 0.383
1.239MetSer: 1.239 ± 0.352
1.859MetThr: 1.859 ± 0.294
0.465MetVal: 0.465 ± 0.149
0.232MetTrp: 0.232 ± 0.194
0.387MetTyr: 0.387 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.099AsnAla: 3.099 ± 0.465
0.31AsnCys: 0.31 ± 0.156
0.775AsnAsp: 0.775 ± 0.31
1.239AsnGlu: 1.239 ± 0.252
0.542AsnPhe: 0.542 ± 0.212
1.782AsnGly: 1.782 ± 0.385
0.465AsnHis: 0.465 ± 0.17
0.31AsnIle: 0.31 ± 0.176
0.232AsnLys: 0.232 ± 0.143
1.394AsnLeu: 1.394 ± 0.284
0.232AsnMet: 0.232 ± 0.116
0.31AsnAsn: 0.31 ± 0.216
1.782AsnPro: 1.782 ± 0.491
0.62AsnGln: 0.62 ± 0.226
1.704AsnArg: 1.704 ± 0.365
0.775AsnSer: 0.775 ± 0.266
1.162AsnThr: 1.162 ± 0.308
1.394AsnVal: 1.394 ± 0.367
0.232AsnTrp: 0.232 ± 0.131
0.465AsnTyr: 0.465 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
9.993ProAla: 9.993 ± 1.16
0.62ProCys: 0.62 ± 0.211
5.423ProAsp: 5.423 ± 0.863
3.873ProGlu: 3.873 ± 0.576
0.775ProPhe: 0.775 ± 0.263
6.507ProGly: 6.507 ± 0.725
1.239ProHis: 1.239 ± 0.449
2.711ProIle: 2.711 ± 0.57
1.239ProLys: 1.239 ± 0.321
5.113ProLeu: 5.113 ± 0.812
1.239ProMet: 1.239 ± 0.323
1.472ProAsn: 1.472 ± 0.343
3.951ProPro: 3.951 ± 0.639
2.169ProGln: 2.169 ± 0.533
4.803ProArg: 4.803 ± 0.732
3.408ProSer: 3.408 ± 0.642
4.261ProThr: 4.261 ± 0.626
5.965ProVal: 5.965 ± 0.738
0.93ProTrp: 0.93 ± 0.309
0.775ProTyr: 0.775 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
5.268GlnAla: 5.268 ± 0.845
0.387GlnCys: 0.387 ± 0.169
1.937GlnAsp: 1.937 ± 0.425
1.007GlnGlu: 1.007 ± 0.271
0.775GlnPhe: 0.775 ± 0.237
3.176GlnGly: 3.176 ± 0.597
0.697GlnHis: 0.697 ± 0.229
0.93GlnIle: 0.93 ± 0.249
0.93GlnLys: 0.93 ± 0.301
5.655GlnLeu: 5.655 ± 0.558
0.775GlnMet: 0.775 ± 0.295
0.542GlnAsn: 0.542 ± 0.262
2.401GlnPro: 2.401 ± 0.448
4.028GlnGln: 4.028 ± 1.969
3.873GlnArg: 3.873 ± 0.669
0.93GlnSer: 0.93 ± 0.291
1.472GlnThr: 1.472 ± 0.297
2.634GlnVal: 2.634 ± 0.438
1.007GlnTrp: 1.007 ± 0.286
0.62GlnTyr: 0.62 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
14.409ArgAla: 14.409 ± 1.41
0.542ArgCys: 0.542 ± 0.209
5.345ArgAsp: 5.345 ± 0.758
4.725ArgGlu: 4.725 ± 0.69
2.014ArgPhe: 2.014 ± 0.449
6.275ArgGly: 6.275 ± 1.215
2.479ArgHis: 2.479 ± 0.632
3.563ArgIle: 3.563 ± 0.465
2.944ArgLys: 2.944 ± 0.515
8.676ArgLeu: 8.676 ± 0.901
1.085ArgMet: 1.085 ± 0.309
1.937ArgAsn: 1.937 ± 0.372
6.739ArgPro: 6.739 ± 0.888
2.711ArgGln: 2.711 ± 0.604
8.211ArgArg: 8.211 ± 1.209
3.718ArgSer: 3.718 ± 0.52
5.19ArgThr: 5.19 ± 0.632
4.958ArgVal: 4.958 ± 0.824
1.317ArgTrp: 1.317 ± 0.32
1.704ArgTyr: 1.704 ± 0.377
0.0ArgXaa: 0.0 ± 0.0
Ser
4.261SerAla: 4.261 ± 0.648
0.232SerCys: 0.232 ± 0.135
3.331SerAsp: 3.331 ± 0.742
1.549SerGlu: 1.549 ± 0.334
1.549SerPhe: 1.549 ± 0.312
4.648SerGly: 4.648 ± 0.464
0.465SerHis: 0.465 ± 0.179
1.162SerIle: 1.162 ± 0.274
1.007SerLys: 1.007 ± 0.255
3.718SerLeu: 3.718 ± 0.63
0.93SerMet: 0.93 ± 0.376
0.775SerAsn: 0.775 ± 0.24
2.711SerPro: 2.711 ± 0.392
0.852SerGln: 0.852 ± 0.295
3.718SerArg: 3.718 ± 0.564
1.782SerSer: 1.782 ± 0.312
4.57SerThr: 4.57 ± 0.669
2.789SerVal: 2.789 ± 0.569
0.93SerTrp: 0.93 ± 0.295
1.007SerTyr: 1.007 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
9.296ThrAla: 9.296 ± 1.037
0.62ThrCys: 0.62 ± 0.305
3.718ThrAsp: 3.718 ± 0.609
3.563ThrGlu: 3.563 ± 0.516
1.937ThrPhe: 1.937 ± 0.431
7.282ThrGly: 7.282 ± 0.808
1.085ThrHis: 1.085 ± 0.297
2.169ThrIle: 2.169 ± 0.433
1.394ThrLys: 1.394 ± 0.342
5.655ThrLeu: 5.655 ± 0.728
0.62ThrMet: 0.62 ± 0.228
1.239ThrAsn: 1.239 ± 0.3
4.493ThrPro: 4.493 ± 0.629
1.704ThrGln: 1.704 ± 0.365
5.19ThrArg: 5.19 ± 0.613
3.021ThrSer: 3.021 ± 0.54
4.338ThrThr: 4.338 ± 1.01
4.493ThrVal: 4.493 ± 0.617
1.472ThrTrp: 1.472 ± 0.373
1.549ThrTyr: 1.549 ± 0.347
0.0ThrXaa: 0.0 ± 0.0
Val
12.24ValAla: 12.24 ± 1.256
0.387ValCys: 0.387 ± 0.2
4.57ValAsp: 4.57 ± 0.583
7.282ValGlu: 7.282 ± 0.845
0.93ValPhe: 0.93 ± 0.41
3.718ValGly: 3.718 ± 0.588
1.085ValHis: 1.085 ± 0.398
2.324ValIle: 2.324 ± 0.459
1.472ValLys: 1.472 ± 0.472
5.268ValLeu: 5.268 ± 0.718
1.394ValMet: 1.394 ± 0.258
1.317ValAsn: 1.317 ± 0.418
4.183ValPro: 4.183 ± 0.608
1.317ValGln: 1.317 ± 0.248
6.585ValArg: 6.585 ± 0.85
3.563ValSer: 3.563 ± 0.529
4.648ValThr: 4.648 ± 0.493
4.648ValVal: 4.648 ± 0.642
1.239ValTrp: 1.239 ± 0.302
1.007ValTyr: 1.007 ± 0.286
0.0ValXaa: 0.0 ± 0.0
Trp
1.859TrpAla: 1.859 ± 0.475
0.232TrpCys: 0.232 ± 0.122
0.775TrpAsp: 0.775 ± 0.289
1.007TrpGlu: 1.007 ± 0.277
0.387TrpPhe: 0.387 ± 0.195
1.317TrpGly: 1.317 ± 0.28
1.085TrpHis: 1.085 ± 0.294
0.542TrpIle: 0.542 ± 0.299
0.542TrpLys: 0.542 ± 0.21
1.627TrpLeu: 1.627 ± 0.431
0.31TrpMet: 0.31 ± 0.144
0.232TrpAsn: 0.232 ± 0.14
0.93TrpPro: 0.93 ± 0.281
0.93TrpGln: 0.93 ± 0.267
1.627TrpArg: 1.627 ± 0.338
0.93TrpSer: 0.93 ± 0.222
1.549TrpThr: 1.549 ± 0.371
1.317TrpVal: 1.317 ± 0.302
0.465TrpTrp: 0.465 ± 0.192
0.232TrpTyr: 0.232 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.641TyrAla: 3.641 ± 0.62
0.155TyrCys: 0.155 ± 0.109
1.937TyrAsp: 1.937 ± 0.426
0.93TyrGlu: 0.93 ± 0.246
0.155TyrPhe: 0.155 ± 0.113
2.866TyrGly: 2.866 ± 0.435
0.465TyrHis: 0.465 ± 0.229
0.542TyrIle: 0.542 ± 0.186
0.077TyrLys: 0.077 ± 0.072
1.239TyrLeu: 1.239 ± 0.296
0.465TyrMet: 0.465 ± 0.146
0.232TyrAsn: 0.232 ± 0.124
0.31TyrPro: 0.31 ± 0.178
0.465TyrGln: 0.465 ± 0.193
2.324TyrArg: 2.324 ± 0.534
0.93TyrSer: 0.93 ± 0.332
1.317TyrThr: 1.317 ± 0.304
1.627TyrVal: 1.627 ± 0.388
0.465TyrTrp: 0.465 ± 0.183
0.155TyrTyr: 0.155 ± 0.097
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12910 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski