Odonata-associated circular virus-4
Average proteome isoelectric point is 9.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UGK2|A0A0B4UGK2_9VIRU Putative capsid protein OS=Odonata-associated circular virus-4 OX=1592124 PE=4 SV=1
MM1 pKa = 8.03 PSTITYY7 pKa = 10.0 IFTINNPTEE16 pKa = 4.7 DD17 pKa = 4.24 DD18 pKa = 3.55 TDD20 pKa = 3.95 KK21 pKa = 11.5 LQLAPGITSIAYY33 pKa = 8.57 QYY35 pKa = 11.15 EE36 pKa = 4.01 RR37 pKa = 11.84 GEE39 pKa = 4.04 TGTRR43 pKa = 11.84 HH44 pKa = 4.47 IQGAIRR50 pKa = 11.84 FARR53 pKa = 11.84 RR54 pKa = 11.84 TTFNQVKK61 pKa = 10.12 RR62 pKa = 11.84 ILPRR66 pKa = 11.84 AHH68 pKa = 6.58 IEE70 pKa = 3.83 AARR73 pKa = 11.84 GTWQQCMDD81 pKa = 3.95 YY82 pKa = 9.28 CTKK85 pKa = 10.9 LEE87 pKa = 4.04 TRR89 pKa = 11.84 EE90 pKa = 4.09 EE91 pKa = 4.95 GPFLLGKK98 pKa = 9.75 CANEE102 pKa = 4.82 DD103 pKa = 3.28 MAPYY107 pKa = 10.02 QRR109 pKa = 11.84 LEE111 pKa = 3.81 EE112 pKa = 4.95 DD113 pKa = 3.34 IKK115 pKa = 11.53 NGATRR120 pKa = 11.84 DD121 pKa = 4.07 DD122 pKa = 3.47 IEE124 pKa = 4.1 NRR126 pKa = 11.84 HH127 pKa = 5.44 FKK129 pKa = 10.66 LYY131 pKa = 10.19 LRR133 pKa = 11.84 HH134 pKa = 5.72 HH135 pKa = 5.77 TAINHH140 pKa = 6.21 ILTFHH145 pKa = 6.5 TKK147 pKa = 9.45 PRR149 pKa = 11.84 SNKK152 pKa = 8.19 PTVTVLWGPTGTGKK166 pKa = 8.16 TRR168 pKa = 11.84 AVVKK172 pKa = 10.13 RR173 pKa = 11.84 ASNRR177 pKa = 11.84 GDD179 pKa = 3.06 SLYY182 pKa = 10.51 IKK184 pKa = 10.28 EE185 pKa = 4.49 RR186 pKa = 11.84 GKK188 pKa = 9.36 WWDD191 pKa = 3.59 GYY193 pKa = 11.17 AGQAICLLDD202 pKa = 4.08 EE203 pKa = 4.91 FYY205 pKa = 11.3 GWLPFGCLLRR215 pKa = 11.84 LLDD218 pKa = 4.37 RR219 pKa = 11.84 YY220 pKa = 9.76 PNRR223 pKa = 11.84 VEE225 pKa = 4.03 YY226 pKa = 10.64 KK227 pKa = 10.4 GGSTQFTSDD236 pKa = 4.41 EE237 pKa = 3.6 IWITSNKK244 pKa = 8.7 EE245 pKa = 3.48 PKK247 pKa = 9.6 DD248 pKa = 3.5 WYY250 pKa = 10.56 KK251 pKa = 11.02 EE252 pKa = 3.97 EE253 pKa = 4.27 YY254 pKa = 9.4 MDD256 pKa = 4.8 ALFRR260 pKa = 11.84 RR261 pKa = 11.84 LDD263 pKa = 4.02 RR264 pKa = 11.84 IVFCDD269 pKa = 3.45 TNVWLLRR276 pKa = 11.84 KK277 pKa = 9.42 IKK279 pKa = 9.69 EE280 pKa = 4.0 QNRR283 pKa = 11.84 PKK285 pKa = 10.55 PYY287 pKa = 9.94 TLNTRR292 pKa = 11.84 QNRR295 pKa = 11.84 LAVRR299 pKa = 11.84 NLTT302 pKa = 3.69
Molecular weight: 35.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.823
IPC2_protein 8.77
IPC_protein 8.712
Toseland 9.238
ProMoST 9.136
Dawson 9.575
Bjellqvist 9.399
Wikipedia 9.765
Rodwell 9.736
Grimsley 9.648
Solomon 9.619
Lehninger 9.575
Nozaki 9.472
DTASelect 9.326
Thurlkill 9.428
EMBOSS 9.706
Sillero 9.575
Patrickios 4.762
IPC_peptide 9.619
IPC2_peptide 8.185
IPC2.peptide.svr19 7.761
Protein with the highest isoelectric point:
>tr|A0A0B4UGK2|A0A0B4UGK2_9VIRU Putative capsid protein OS=Odonata-associated circular virus-4 OX=1592124 PE=4 SV=1
MM1 pKa = 7.41 VYY3 pKa = 10.04 RR4 pKa = 11.84 RR5 pKa = 11.84 ANGGVSYY12 pKa = 10.53 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 LRR18 pKa = 11.84 SSRR21 pKa = 11.84 VRR23 pKa = 11.84 RR24 pKa = 11.84 STSRR28 pKa = 11.84 AKK30 pKa = 10.07 YY31 pKa = 7.67 SRR33 pKa = 11.84 RR34 pKa = 11.84 MRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 SGRR42 pKa = 11.84 YY43 pKa = 7.69 GYY45 pKa = 10.38 RR46 pKa = 11.84 RR47 pKa = 11.84 AGGSGYY53 pKa = 10.1 GFRR56 pKa = 11.84 VRR58 pKa = 11.84 SRR60 pKa = 11.84 GGLPTRR66 pKa = 11.84 LTTRR70 pKa = 11.84 WSTVYY75 pKa = 9.74 TYY77 pKa = 10.92 PDD79 pKa = 3.62 GFEE82 pKa = 5.95 HH83 pKa = 6.21 IDD85 pKa = 2.96 KK86 pKa = 9.78 WIEE89 pKa = 3.96 VVTSQTAPTGMEE101 pKa = 3.63 NFFRR105 pKa = 11.84 EE106 pKa = 4.81 FKK108 pKa = 10.25 PLYY111 pKa = 9.62 AEE113 pKa = 4.2 VYY115 pKa = 7.4 VRR117 pKa = 11.84 RR118 pKa = 11.84 WRR120 pKa = 11.84 AVGADD125 pKa = 3.75 LQIVSTSEE133 pKa = 3.8 GFRR136 pKa = 11.84 LLSSVTIMLGIFRR149 pKa = 11.84 IVLVFRR155 pKa = 11.84 FSILVSVPKK164 pKa = 10.74 LSRR167 pKa = 11.84 FLSTGWVGGIVKK179 pKa = 10.15 QKK181 pKa = 10.3 SYY183 pKa = 11.01 RR184 pKa = 11.84 LAYY187 pKa = 10.35 FSDD190 pKa = 3.67 KK191 pKa = 11.19 DD192 pKa = 3.81 AVPDD196 pKa = 3.55 KK197 pKa = 10.82 ATAQPYY203 pKa = 9.22 AASAPWMPMGSTWGWKK219 pKa = 9.61 LGCLHH224 pKa = 7.18 SNLEE228 pKa = 4.19 WKK230 pKa = 10.75 VFDD233 pKa = 4.37 ANSFEE238 pKa = 4.6 FVAKK242 pKa = 8.38 FHH244 pKa = 5.8 WAFRR248 pKa = 11.84 GRR250 pKa = 11.84 KK251 pKa = 8.12 PLGVDD256 pKa = 2.95 TDD258 pKa = 4.02 YY259 pKa = 11.77 NGYY262 pKa = 9.67 IEE264 pKa = 5.2 GDD266 pKa = 3.86 VVIPTLDD273 pKa = 3.45 HH274 pKa = 7.49 DD275 pKa = 4.85 PNN277 pKa = 4.07
Molecular weight: 31.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 10.043
IPC_protein 10.994
Toseland 10.833
ProMoST 10.789
Dawson 10.95
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.008
Grimsley 11.023
Solomon 11.14
Lehninger 11.082
Nozaki 10.818
DTASelect 10.76
Thurlkill 10.862
EMBOSS 11.272
Sillero 10.891
Patrickios 10.657
IPC_peptide 11.14
IPC2_peptide 9.882
IPC2.peptide.svr19 8.247
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
579
277
302
289.5
33.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.872 ± 0.174
1.209 ± 0.557
5.009 ± 0.445
4.836 ± 1.043
4.663 ± 0.732
7.599 ± 0.937
1.9 ± 0.3
5.009 ± 0.919
5.354 ± 0.672
7.599 ± 0.724
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.727 ± 0.289
3.8 ± 1.074
4.491 ± 0.104
2.591 ± 0.754
11.226 ± 1.164
5.699 ± 2.186
7.772 ± 1.312
5.699 ± 1.949
2.936 ± 0.206
5.009 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here