Infectious pancreatic necrosis virus (strain Jasper) (IPNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Aquabirnavirus; Infectious pancreatic necrosis virus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P22173|RDRP_IPNVJ RNA-directed RNA polymerase OS=Infectious pancreatic necrosis virus (strain Jasper) OX=11003 GN=VP1 PE=1 SV=1
MM1 pKa = 6.85STSKK5 pKa = 9.92ATATYY10 pKa = 9.68LRR12 pKa = 11.84SIMLPEE18 pKa = 4.78NGPASIPDD26 pKa = 5.04DD27 pKa = 3.12ITEE30 pKa = 3.99RR31 pKa = 11.84HH32 pKa = 5.89ILKK35 pKa = 10.07QEE37 pKa = 3.84TSSYY41 pKa = 9.6NLEE44 pKa = 4.05VSEE47 pKa = 4.7SGSGLLVCFPGAPGSRR63 pKa = 11.84VGAHH67 pKa = 5.62YY68 pKa = 10.33RR69 pKa = 11.84WNLNQTALEE78 pKa = 3.92FDD80 pKa = 3.2QWLEE84 pKa = 3.86TSQDD88 pKa = 3.07LKK90 pKa = 11.21KK91 pKa = 10.75AFNYY95 pKa = 9.81GRR97 pKa = 11.84LISRR101 pKa = 11.84KK102 pKa = 9.31YY103 pKa = 10.36DD104 pKa = 3.38IQSSTLPAGLYY115 pKa = 10.34ALNGTLNAATFEE127 pKa = 4.46GSLSEE132 pKa = 4.62VEE134 pKa = 4.23SLTYY138 pKa = 10.53NSLMSLTTNPQDD150 pKa = 3.31KK151 pKa = 10.9VNNQLVTKK159 pKa = 10.33GITVLNLPTGFDD171 pKa = 3.17KK172 pKa = 10.97PYY174 pKa = 10.8VRR176 pKa = 11.84LEE178 pKa = 4.3DD179 pKa = 3.78EE180 pKa = 4.8TPQGPQSMNGARR192 pKa = 11.84MRR194 pKa = 11.84CTAAIAPRR202 pKa = 11.84RR203 pKa = 11.84YY204 pKa = 10.22EE205 pKa = 3.45IDD207 pKa = 3.67LPSEE211 pKa = 4.08RR212 pKa = 11.84LPTVAATGTPTTIYY226 pKa = 10.0EE227 pKa = 4.25GNADD231 pKa = 3.82IVNSTAVTGDD241 pKa = 2.71ITFQLEE247 pKa = 4.12AEE249 pKa = 4.54PVNEE253 pKa = 3.99TRR255 pKa = 11.84FDD257 pKa = 4.63FILQFLGLDD266 pKa = 3.17NDD268 pKa = 4.13VPVVTVTSSTLVTADD283 pKa = 3.27NYY285 pKa = 10.84RR286 pKa = 11.84GASAKK291 pKa = 8.3FTQSIPTEE299 pKa = 4.46MITKK303 pKa = 9.77PITRR307 pKa = 11.84VKK309 pKa = 10.35LAYY312 pKa = 9.8QLNQQTAIANAATLGAKK329 pKa = 10.23GPASVSFSSGNGNVPGVLRR348 pKa = 11.84PITLVAYY355 pKa = 9.89EE356 pKa = 4.46KK357 pKa = 7.78MTPQSILTVAGVSNYY372 pKa = 9.47EE373 pKa = 4.59LIPNPDD379 pKa = 3.58LLKK382 pKa = 11.45NMVTKK387 pKa = 10.51YY388 pKa = 10.85GKK390 pKa = 10.23YY391 pKa = 10.23DD392 pKa = 3.7PEE394 pKa = 4.22GLNYY398 pKa = 10.95AKK400 pKa = 10.06MILSHH405 pKa = 7.46RR406 pKa = 11.84EE407 pKa = 3.56EE408 pKa = 4.78LDD410 pKa = 2.89IRR412 pKa = 11.84TVWRR416 pKa = 11.84TEE418 pKa = 3.79EE419 pKa = 3.97YY420 pKa = 10.52KK421 pKa = 10.87EE422 pKa = 3.62RR423 pKa = 11.84TRR425 pKa = 11.84AFKK428 pKa = 10.48EE429 pKa = 3.83ITDD432 pKa = 4.1FTSDD436 pKa = 4.33LPTSKK441 pKa = 10.55AWGWRR446 pKa = 11.84DD447 pKa = 3.21LVRR450 pKa = 11.84GIRR453 pKa = 11.84KK454 pKa = 8.6VAAPVLSTLFPMAAPLIGAADD475 pKa = 3.69QFIGDD480 pKa = 3.86LTKK483 pKa = 10.61TNSAGGRR490 pKa = 11.84YY491 pKa = 9.11LSHH494 pKa = 6.97AAGGRR499 pKa = 11.84YY500 pKa = 8.91HH501 pKa = 7.51DD502 pKa = 5.76VMDD505 pKa = 3.94SWASGSEE512 pKa = 3.51AGSYY516 pKa = 10.26SKK518 pKa = 10.62HH519 pKa = 5.68LKK521 pKa = 8.54TRR523 pKa = 11.84LEE525 pKa = 4.22SNNYY529 pKa = 9.89EE530 pKa = 4.21EE531 pKa = 5.46VEE533 pKa = 4.19LPKK536 pKa = 9.21PTKK539 pKa = 10.28GVIFPVVHH547 pKa = 5.68TVEE550 pKa = 4.43SAPGEE555 pKa = 4.18AFGSLVVVIPEE566 pKa = 5.11AYY568 pKa = 9.5PEE570 pKa = 4.38LLDD573 pKa = 4.73PNQQVLSYY581 pKa = 9.38FKK583 pKa = 11.03NDD585 pKa = 3.51TGCVWGIGEE594 pKa = 5.44DD595 pKa = 3.57IPFEE599 pKa = 5.1GDD601 pKa = 3.46DD602 pKa = 3.54MCYY605 pKa = 9.28TALPLKK611 pKa = 9.86EE612 pKa = 3.89IKK614 pKa = 10.42RR615 pKa = 11.84NGNIVVEE622 pKa = 4.81KK623 pKa = 10.34IFAGPAMGPSSQLALSLLVNDD644 pKa = 4.18IDD646 pKa = 3.83EE647 pKa = 5.54GIPRR651 pKa = 11.84MVFTGEE657 pKa = 3.79IADD660 pKa = 4.48DD661 pKa = 4.32EE662 pKa = 4.63EE663 pKa = 4.8TVIPICGVDD672 pKa = 3.34IKK674 pKa = 11.15AIAAHH679 pKa = 5.38EE680 pKa = 4.63HH681 pKa = 5.69GLPLIGCQPGVDD693 pKa = 3.11EE694 pKa = 4.62MVANTSLASHH704 pKa = 7.66LIQGGALPVQKK715 pKa = 10.73AQGACRR721 pKa = 11.84RR722 pKa = 11.84IKK724 pKa = 10.9YY725 pKa = 9.71LGQLMRR731 pKa = 11.84TTASGMDD738 pKa = 3.61AEE740 pKa = 4.72LQGLLQATMARR751 pKa = 11.84AKK753 pKa = 9.47EE754 pKa = 4.15VKK756 pKa = 9.93DD757 pKa = 3.6AEE759 pKa = 4.44VFKK762 pKa = 10.84LLKK765 pKa = 10.54LMSWTRR771 pKa = 11.84KK772 pKa = 9.62NDD774 pKa = 3.71LTDD777 pKa = 5.05HH778 pKa = 5.96MYY780 pKa = 10.29EE781 pKa = 3.96WSKK784 pKa = 11.28EE785 pKa = 3.95DD786 pKa = 3.38PDD788 pKa = 4.52AIKK791 pKa = 10.46FGRR794 pKa = 11.84LVSTPPKK801 pKa = 9.86HH802 pKa = 5.49QEE804 pKa = 3.87KK805 pKa = 10.39PKK807 pKa = 11.16GPDD810 pKa = 2.69QHH812 pKa = 6.27TAQEE816 pKa = 4.12AKK818 pKa = 9.17ATRR821 pKa = 11.84ISLDD825 pKa = 3.29AVKK828 pKa = 10.64AGADD832 pKa = 3.54FASPEE837 pKa = 4.06WIAEE841 pKa = 3.88NNYY844 pKa = 10.01RR845 pKa = 11.84GPSPGQFKK853 pKa = 11.0YY854 pKa = 11.47YY855 pKa = 9.51MITGRR860 pKa = 11.84VPNPGEE866 pKa = 3.84EE867 pKa = 4.23YY868 pKa = 9.96EE869 pKa = 4.56DD870 pKa = 4.02YY871 pKa = 10.81VRR873 pKa = 11.84KK874 pKa = 9.51PITRR878 pKa = 11.84PTDD881 pKa = 2.99MDD883 pKa = 4.24KK884 pKa = 10.54IRR886 pKa = 11.84RR887 pKa = 11.84LANSVYY893 pKa = 10.06GLPHH897 pKa = 6.31QEE899 pKa = 3.81PAPDD903 pKa = 3.89DD904 pKa = 4.21FYY906 pKa = 11.43QAVVEE911 pKa = 4.34VFAEE915 pKa = 4.05NGGRR919 pKa = 11.84GPDD922 pKa = 3.4QDD924 pKa = 3.5QMQDD928 pKa = 3.21LRR930 pKa = 11.84DD931 pKa = 3.54LARR934 pKa = 11.84QMKK937 pKa = 9.58RR938 pKa = 11.84RR939 pKa = 11.84PRR941 pKa = 11.84PAEE944 pKa = 3.49TRR946 pKa = 11.84RR947 pKa = 11.84QTKK950 pKa = 8.26TPPRR954 pKa = 11.84AATSSGSRR962 pKa = 11.84FTPSGDD968 pKa = 3.49DD969 pKa = 3.82GEE971 pKa = 4.6VV972 pKa = 2.82

Molecular weight: 106.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P22931|VP5_IPNVJ Protein VP5 OS=Infectious pancreatic necrosis virus (strain Jasper) OX=11003 GN=VP5 PE=3 SV=1
MM1 pKa = 7.71AKK3 pKa = 10.29ALSNKK8 pKa = 6.69PTNILIYY15 pKa = 9.45MNHH18 pKa = 5.76EE19 pKa = 4.53HH20 pKa = 6.41IQGNRR25 pKa = 11.84NLLEE29 pKa = 3.32IHH31 pKa = 5.78YY32 pKa = 10.24ASRR35 pKa = 11.84EE36 pKa = 4.02WASKK40 pKa = 10.27HH41 pKa = 5.16SGRR44 pKa = 11.84HH45 pKa = 3.75NRR47 pKa = 11.84EE48 pKa = 3.92AYY50 pKa = 8.14TKK52 pKa = 10.12TRR54 pKa = 11.84DD55 pKa = 3.76LVIQLRR61 pKa = 11.84GIRR64 pKa = 11.84IRR66 pKa = 11.84KK67 pKa = 6.51WASCLLPRR75 pKa = 11.84SSWIQGRR82 pKa = 11.84CPLQVEE88 pKa = 4.5SEE90 pKa = 4.15PDD92 pKa = 3.2GTRR95 pKa = 11.84IRR97 pKa = 11.84PVARR101 pKa = 11.84DD102 pKa = 3.21VTGPKK107 pKa = 10.08EE108 pKa = 4.45GIQLRR113 pKa = 11.84EE114 pKa = 3.66TDD116 pKa = 3.56LTEE119 pKa = 4.03IRR121 pKa = 11.84HH122 pKa = 6.1PEE124 pKa = 4.03LNPSRR129 pKa = 11.84WSVCTQWDD137 pKa = 3.64PEE139 pKa = 4.23RR140 pKa = 11.84CHH142 pKa = 7.07LRR144 pKa = 11.84RR145 pKa = 11.84KK146 pKa = 9.69SVV148 pKa = 2.76

Molecular weight: 17.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1965

148

972

655.0

72.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.99 ± 0.804

0.712 ± 0.441

5.802 ± 0.541

6.463 ± 0.244

2.646 ± 0.577

6.565 ± 0.697

1.527 ± 0.699

5.089 ± 0.513

5.903 ± 0.624

9.415 ± 0.711

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.278

4.682 ± 0.316

6.768 ± 0.16

3.868 ± 0.054

5.7 ± 1.55

6.412 ± 0.274

7.786 ± 0.561

5.344 ± 0.586

1.476 ± 0.471

3.308 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski