candidate division MSBL1 archaeon SCGC-AAA385M02

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VQA6|A0A133VQA6_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA385M02 OX=1698287 GN=AKJ59_00730 PE=4 SV=1
MM1 pKa = 6.63VTYY4 pKa = 10.26LPSSAQTSITIEE16 pKa = 4.05EE17 pKa = 4.85LEE19 pKa = 4.27VSTDD23 pKa = 3.6LTISAPISAVEE34 pKa = 4.13GEE36 pKa = 4.46SFSVSGILVRR46 pKa = 11.84VDD48 pKa = 3.04TGEE51 pKa = 4.83PIPNQTISITYY62 pKa = 9.0NGTAVGSGTTGVDD75 pKa = 2.85GDD77 pKa = 4.09YY78 pKa = 10.79LITISIPDD86 pKa = 4.07FGTYY90 pKa = 8.98TLTAEE95 pKa = 4.28FAGVSGLRR103 pKa = 11.84ASEE106 pKa = 3.93ARR108 pKa = 11.84SGISVGFIEE117 pKa = 4.73MPNWLNILVPLIVGTAVYY135 pKa = 10.62KK136 pKa = 11.01LGGGG140 pKa = 3.56

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VR97|A0A133VR97_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA385M02 OX=1698287 GN=AKJ59_00070 PE=4 SV=1
MM1 pKa = 7.6SGNIYY6 pKa = 9.28DD7 pKa = 3.87TMKK10 pKa = 10.64SILGGGVPGSQPKK23 pKa = 10.35NGLIGGGANSNSSSGMEE40 pKa = 4.12GGQPRR45 pKa = 11.84GMEE48 pKa = 3.73RR49 pKa = 11.84SILRR53 pKa = 11.84RR54 pKa = 11.84AFNNQAIYY62 pKa = 9.92KK63 pKa = 9.91ANGGQKK69 pKa = 10.07HH70 pKa = 5.6SALNLAGKK78 pKa = 8.55ATLSFRR84 pKa = 11.84AAMNAGDD91 pKa = 4.16PLHH94 pKa = 7.45RR95 pKa = 11.84YY96 pKa = 7.2QQSCGGSNQVNNVRR110 pKa = 11.84RR111 pKa = 11.84SGSGVAKK118 pKa = 10.34SSVGGVSDD126 pKa = 4.27SNCKK130 pKa = 10.23KK131 pKa = 8.96STSFGGLVYY140 pKa = 10.16VTGKK144 pKa = 10.2DD145 pKa = 3.67VNPLQSGNHH154 pKa = 5.88RR155 pKa = 11.84YY156 pKa = 10.16VYY158 pKa = 10.52DD159 pKa = 3.92GSDD162 pKa = 3.18YY163 pKa = 11.45TRR165 pKa = 11.84FKK167 pKa = 11.02KK168 pKa = 10.35LASQNRR174 pKa = 11.84NYY176 pKa = 10.54NDD178 pKa = 3.23KK179 pKa = 11.39SFGGSNNGAYY189 pKa = 10.01SALNRR194 pKa = 11.84VRR196 pKa = 11.84RR197 pKa = 3.82

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

33380

59

1385

214.0

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.473 ± 0.317

1.372 ± 0.105

6.096 ± 0.174

7.397 ± 0.258

3.847 ± 0.14

6.093 ± 0.242

2.097 ± 0.112

7.313 ± 0.236

9.464 ± 0.35

7.442 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.927 ± 0.109

6.525 ± 0.26

3.4 ± 0.14

3.706 ± 0.179

4.158 ± 0.172

6.567 ± 0.198

5.512 ± 0.204

5.632 ± 0.17

1.174 ± 0.087

4.805 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski