Gordonia phage Hotorobo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Montyvirus; unclassified Montyvirus; Gordonia virus Hotorobo

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142K8G8|A0A142K8G8_9CAUD Uncharacterized protein OS=Gordonia phage Hotorobo OX=1821554 GN=109 PE=4 SV=1
MM1 pKa = 7.69NYY3 pKa = 9.02TVTYY7 pKa = 9.03YY8 pKa = 10.3PPADD12 pKa = 3.77ADD14 pKa = 3.82GQPFEE19 pKa = 4.5SRR21 pKa = 11.84PVPTLDD27 pKa = 3.71DD28 pKa = 4.19ALDD31 pKa = 3.51IVMDD35 pKa = 4.02DD36 pKa = 4.38CEE38 pKa = 4.66RR39 pKa = 11.84EE40 pKa = 4.34SVGITGIEE48 pKa = 3.79RR49 pKa = 11.84EE50 pKa = 4.22VDD52 pKa = 2.98PEE54 pKa = 3.86TRR56 pKa = 11.84IVHH59 pKa = 5.98WGVVCDD65 pKa = 5.24DD66 pKa = 3.7GASYY70 pKa = 10.89VIVKK74 pKa = 9.54LL75 pKa = 3.79

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142K867|A0A142K867_9CAUD Uncharacterized protein OS=Gordonia phage Hotorobo OX=1821554 GN=7 PE=4 SV=1
MM1 pKa = 7.87PNRR4 pKa = 11.84RR5 pKa = 11.84DD6 pKa = 3.18KK7 pKa = 11.28FRR9 pKa = 11.84GNRR12 pKa = 11.84IEE14 pKa = 3.73IRR16 pKa = 11.84VRR18 pKa = 11.84AQRR21 pKa = 11.84DD22 pKa = 2.97LDD24 pKa = 3.67GFVIHH29 pKa = 7.21CYY31 pKa = 10.11DD32 pKa = 3.32NRR34 pKa = 11.84GMLVATGCYY43 pKa = 9.13HH44 pKa = 7.1KK45 pKa = 11.33SNVTMTATRR54 pKa = 11.84AASLALGVPEE64 pKa = 4.04GRR66 pKa = 11.84IKK68 pKa = 10.52ISRR71 pKa = 11.84LEE73 pKa = 3.96YY74 pKa = 10.66AA75 pKa = 4.4

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

24185

30

3327

223.9

24.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.659 ± 0.975

1.03 ± 0.149

6.177 ± 0.282

7.042 ± 0.369

3.453 ± 0.098

7.806 ± 0.238

1.724 ± 0.172

5.222 ± 0.171

5.355 ± 0.225

7.335 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.15

3.978 ± 0.111

5.293 ± 0.348

3.928 ± 0.173

5.516 ± 0.362

5.805 ± 0.213

6.119 ± 0.24

6.905 ± 0.182

1.753 ± 0.105

3.101 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski