Sena Madureira virus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1E6|A0A0D3R1E6_9RHAB Phosphoprotein OS=Sena Madureira virus OX=1272957 PE=4 SV=1
MM1 pKa = 7.68 SYY3 pKa = 10.91 YY4 pKa = 10.81 LEE6 pKa = 4.19 EE7 pKa = 4.46 LKK9 pKa = 11.39 SNDD12 pKa = 2.99 VNMEE16 pKa = 4.04 EE17 pKa = 4.8 VPFDD21 pKa = 3.31 TTAYY25 pKa = 8.92 NTFEE29 pKa = 3.85 VDD31 pKa = 4.99 AIPEE35 pKa = 4.27 GLPQWEE41 pKa = 4.46 IKK43 pKa = 9.88 RR44 pKa = 11.84 RR45 pKa = 11.84 QIDD48 pKa = 3.84 NILGALSQLGVRR60 pKa = 11.84 CDD62 pKa = 3.76 YY63 pKa = 10.66 LTDD66 pKa = 3.92 DD67 pKa = 3.77 VNSIRR72 pKa = 11.84 FYY74 pKa = 10.33 ITLDD78 pKa = 3.38 PQLYY82 pKa = 9.72 SYY84 pKa = 10.94 EE85 pKa = 4.12 VDD87 pKa = 3.58 PEE89 pKa = 4.58 DD90 pKa = 4.32 NFIIFEE96 pKa = 4.21 NEE98 pKa = 3.46 QSEE101 pKa = 4.74 DD102 pKa = 3.68 EE103 pKa = 5.42 DD104 pKa = 3.81 EE105 pKa = 4.32 FLCFIEE111 pKa = 4.8 EE112 pKa = 3.96 WDD114 pKa = 3.77 FF115 pKa = 3.97
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 3.592
IPC_protein 3.554
Toseland 3.363
ProMoST 3.719
Dawson 3.541
Bjellqvist 3.694
Wikipedia 3.465
Rodwell 3.389
Grimsley 3.274
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.834
Thurlkill 3.414
EMBOSS 3.478
Sillero 3.668
Patrickios 0.54
IPC_peptide 3.516
IPC2_peptide 3.656
IPC2.peptide.svr19 3.673
Protein with the highest isoelectric point:
>tr|A0A0D3R1U0|A0A0D3R1U0_9RHAB Nucleocapsid protein OS=Sena Madureira virus OX=1272957 PE=4 SV=1
MM1 pKa = 7.27 SWIFGNRR8 pKa = 11.84 DD9 pKa = 2.82 RR10 pKa = 11.84 TQLFDD15 pKa = 3.5 SYY17 pKa = 11.61 LNGSSSDD24 pKa = 3.48 NTSQQDD30 pKa = 3.53 LPTYY34 pKa = 10.35 QIVGDD39 pKa = 4.33 FNLILNKK46 pKa = 10.12 ALQSKK51 pKa = 10.05 EE52 pKa = 4.09 DD53 pKa = 3.6 YY54 pKa = 10.74 KK55 pKa = 11.27 DD56 pKa = 3.19 LHH58 pKa = 6.18 KK59 pKa = 10.95 QLVEE63 pKa = 4.23 NYY65 pKa = 7.95 TGSYY69 pKa = 7.85 SAKK72 pKa = 9.73 PIVILLVRR80 pKa = 11.84 LTALTLRR87 pKa = 11.84 KK88 pKa = 10.25 DD89 pKa = 2.94 KK90 pKa = 10.74 TEE92 pKa = 4.23 RR93 pKa = 11.84 DD94 pKa = 2.99 NHH96 pKa = 6.27 YY97 pKa = 10.64 YY98 pKa = 10.24 SGSFAGIIRR107 pKa = 11.84 LGISVDD113 pKa = 4.01 LPTPPDD119 pKa = 3.15 INRR122 pKa = 11.84 YY123 pKa = 9.98 SFDD126 pKa = 3.24 HH127 pKa = 6.73 SINNSEE133 pKa = 3.68 YY134 pKa = 8.47 RR135 pKa = 11.84 GRR137 pKa = 11.84 YY138 pKa = 7.76 FGSVAYY144 pKa = 8.02 TKK146 pKa = 10.57 RR147 pKa = 11.84 RR148 pKa = 11.84 GEE150 pKa = 4.1 SVRR153 pKa = 11.84 NHH155 pKa = 4.38 STYY158 pKa = 10.59 IKK160 pKa = 10.65 NLARR164 pKa = 11.84 TNPLTFEE171 pKa = 5.08 DD172 pKa = 5.52 FDD174 pKa = 4.92 LSDD177 pKa = 3.83 YY178 pKa = 11.0 KK179 pKa = 11.24 FEE181 pKa = 4.39 INKK184 pKa = 9.4 RR185 pKa = 11.84 HH186 pKa = 5.91 ILISQKK192 pKa = 11.04
Molecular weight: 22.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.605
IPC2_protein 8.682
IPC_protein 8.682
Toseland 9.063
ProMoST 9.092
Dawson 9.399
Bjellqvist 9.136
Wikipedia 9.633
Rodwell 9.502
Grimsley 9.516
Solomon 9.472
Lehninger 9.428
Nozaki 8.99
DTASelect 9.151
Thurlkill 9.18
EMBOSS 9.487
Sillero 9.297
Patrickios 4.393
IPC_peptide 9.458
IPC2_peptide 7.746
IPC2.peptide.svr19 7.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3683
99
2098
526.1
60.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.824 ± 0.555
2.009 ± 0.338
6.109 ± 0.656
6.734 ± 0.714
4.643 ± 0.262
5.783 ± 0.487
1.982 ± 0.339
9.449 ± 0.724
7.44 ± 0.484
9.367 ± 0.505
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.525 ± 0.335
6.326 ± 0.53
3.72 ± 0.225
2.987 ± 0.369
4.507 ± 0.422
7.657 ± 0.559
5.349 ± 0.398
4.724 ± 0.43
1.792 ± 0.312
4.073 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here