Putridiphycobacter roseus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Putridiphycobacter

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W1N1X8|A0A2W1N1X8_9FLAO Glycos_transf_1 domain-containing protein OS=Putridiphycobacter roseus OX=2219161 GN=DNU06_02070 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.32RR3 pKa = 11.84ITILTLLFFISSYY16 pKa = 9.93TKK18 pKa = 9.74LSAAIVYY25 pKa = 9.53TDD27 pKa = 3.61INDD30 pKa = 3.2VTLQIGGAIDD40 pKa = 3.52IDD42 pKa = 4.0FDD44 pKa = 4.98GDD46 pKa = 3.77GLPEE50 pKa = 4.05FTFEE54 pKa = 4.11DD55 pKa = 3.55QGFGGQVEE63 pKa = 4.31PGVIFNNVNEE73 pKa = 4.44HH74 pKa = 5.38FTTVSLAEE82 pKa = 3.77WDD84 pKa = 4.32VIQGVPLNTTIDD96 pKa = 3.73MNSNWFDD103 pKa = 3.21QGDD106 pKa = 4.1AYY108 pKa = 11.29VDD110 pKa = 4.12PGWGTTLFPTTDD122 pKa = 3.3TYY124 pKa = 11.09IGASFEE130 pKa = 4.54VGGNTHH136 pKa = 6.25YY137 pKa = 11.14GWIFVNWDD145 pKa = 3.23GNGTFVIKK153 pKa = 10.43SYY155 pKa = 10.88AYY157 pKa = 7.87EE158 pKa = 4.23TDD160 pKa = 3.14NNTPILAGATNANTNVMVNSITLSTANGEE189 pKa = 4.25TTVAVGDD196 pKa = 4.18DD197 pKa = 4.16LQVSAAILPLNASNTDD213 pKa = 3.58VTWTIVNGSGTATIDD228 pKa = 3.67ANGLLSALTLGTITVVATANDD249 pKa = 4.03GSGITAQIQINITEE263 pKa = 4.11VTPIVDD269 pKa = 4.05NGNTDD274 pKa = 4.71PILDD278 pKa = 4.25PGSPLSEE285 pKa = 4.14VEE287 pKa = 3.92SSVIYY292 pKa = 10.27VPSVFTPNAGNINKK306 pKa = 8.43TFEE309 pKa = 4.27VIADD313 pKa = 3.87NIEE316 pKa = 3.98DD317 pKa = 3.81FKK319 pKa = 11.24MIIYY323 pKa = 9.28NRR325 pKa = 11.84WGEE328 pKa = 4.23VVYY331 pKa = 10.63VSYY334 pKa = 10.45DD335 pKa = 3.31QRR337 pKa = 11.84VAWDD341 pKa = 3.76GSYY344 pKa = 10.87AGRR347 pKa = 11.84SNPNEE352 pKa = 3.85MYY354 pKa = 9.85VWKK357 pKa = 10.48INYY360 pKa = 9.1RR361 pKa = 11.84DD362 pKa = 3.52AKK364 pKa = 8.75GQKK367 pKa = 9.75NEE369 pKa = 4.44IIGQVLVLII378 pKa = 5.0

Molecular weight: 40.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W1NQV2|A0A2W1NQV2_9FLAO Uncharacterized protein OS=Putridiphycobacter roseus OX=2219161 GN=DNU06_09880 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSLRR11 pKa = 11.84KK12 pKa = 9.41RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 10.15HH17 pKa = 3.94GFRR20 pKa = 11.84TRR22 pKa = 11.84MATKK26 pKa = 10.27NGRR29 pKa = 11.84AVIKK33 pKa = 10.2ARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.28GRR40 pKa = 11.84KK41 pKa = 8.49KK42 pKa = 10.61LSISSEE48 pKa = 3.94RR49 pKa = 11.84LAKK52 pKa = 10.33RR53 pKa = 3.3

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3484

0

3484

1212977

25

3626

348.2

39.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.418 ± 0.04

0.885 ± 0.017

5.379 ± 0.038

6.056 ± 0.046

5.34 ± 0.035

6.465 ± 0.049

1.789 ± 0.018

8.345 ± 0.044

7.531 ± 0.067

9.321 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.018

6.615 ± 0.046

3.358 ± 0.022

3.438 ± 0.022

2.862 ± 0.028

6.59 ± 0.033

5.946 ± 0.047

6.095 ± 0.033

1.068 ± 0.014

4.282 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski