Amino acid dipepetide frequency for Pseudomonas phage Dobby

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.007AlaAla: 14.007 ± 1.381
1.152AlaCys: 1.152 ± 0.3
8.156AlaAsp: 8.156 ± 0.679
6.028AlaGlu: 6.028 ± 0.864
2.926AlaPhe: 2.926 ± 0.509
9.22AlaGly: 9.22 ± 1.181
2.394AlaHis: 2.394 ± 0.442
5.94AlaIle: 5.94 ± 0.546
3.103AlaLys: 3.103 ± 0.457
11.613AlaLeu: 11.613 ± 0.868
2.482AlaMet: 2.482 ± 0.48
3.989AlaAsn: 3.989 ± 0.487
4.344AlaPro: 4.344 ± 0.678
6.028AlaGln: 6.028 ± 0.678
7.092AlaArg: 7.092 ± 0.76
5.762AlaSer: 5.762 ± 0.701
6.383AlaThr: 6.383 ± 0.982
7.535AlaVal: 7.535 ± 0.949
1.507AlaTrp: 1.507 ± 0.395
3.723AlaTyr: 3.723 ± 0.585
0.0AlaXaa: 0.0 ± 0.0
Cys
0.798CysAla: 0.798 ± 0.22
0.266CysCys: 0.266 ± 0.162
0.443CysAsp: 0.443 ± 0.259
0.532CysGlu: 0.532 ± 0.212
0.532CysPhe: 0.532 ± 0.243
1.507CysGly: 1.507 ± 0.461
0.177CysHis: 0.177 ± 0.12
0.443CysIle: 0.443 ± 0.215
0.355CysLys: 0.355 ± 0.173
0.798CysLeu: 0.798 ± 0.259
0.355CysMet: 0.355 ± 0.169
0.266CysAsn: 0.266 ± 0.145
0.532CysPro: 0.532 ± 0.225
0.266CysGln: 0.266 ± 0.134
0.532CysArg: 0.532 ± 0.24
0.709CysSer: 0.709 ± 0.199
0.532CysThr: 0.532 ± 0.216
0.355CysVal: 0.355 ± 0.192
0.443CysTrp: 0.443 ± 0.197
0.266CysTyr: 0.266 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
6.206AspAla: 6.206 ± 0.668
0.532AspCys: 0.532 ± 0.23
2.482AspAsp: 2.482 ± 0.408
3.103AspGlu: 3.103 ± 0.567
1.684AspPhe: 1.684 ± 0.293
4.521AspGly: 4.521 ± 0.715
1.241AspHis: 1.241 ± 0.273
3.191AspIle: 3.191 ± 0.484
2.394AspLys: 2.394 ± 0.487
7.447AspLeu: 7.447 ± 0.761
0.975AspMet: 0.975 ± 0.287
1.95AspAsn: 1.95 ± 0.361
3.28AspPro: 3.28 ± 0.82
3.546AspGln: 3.546 ± 0.546
4.078AspArg: 4.078 ± 0.538
3.457AspSer: 3.457 ± 0.431
3.28AspThr: 3.28 ± 0.612
2.66AspVal: 2.66 ± 0.449
1.418AspTrp: 1.418 ± 0.424
2.394AspTyr: 2.394 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
6.294GluAla: 6.294 ± 0.811
1.064GluCys: 1.064 ± 0.316
2.66GluAsp: 2.66 ± 0.471
2.571GluGlu: 2.571 ± 0.602
2.394GluPhe: 2.394 ± 0.602
2.748GluGly: 2.748 ± 0.563
1.418GluHis: 1.418 ± 0.379
3.191GluIle: 3.191 ± 0.599
2.748GluLys: 2.748 ± 0.455
7.358GluLeu: 7.358 ± 0.812
0.975GluMet: 0.975 ± 0.268
1.95GluAsn: 1.95 ± 0.469
2.66GluPro: 2.66 ± 0.537
2.482GluGln: 2.482 ± 0.409
4.167GluArg: 4.167 ± 0.573
3.28GluSer: 3.28 ± 0.732
2.216GluThr: 2.216 ± 0.432
3.812GluVal: 3.812 ± 0.633
1.152GluTrp: 1.152 ± 0.299
1.507GluTyr: 1.507 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
3.103PheAla: 3.103 ± 0.511
0.266PheCys: 0.266 ± 0.132
2.748PheAsp: 2.748 ± 0.467
1.95PheGlu: 1.95 ± 0.455
1.152PhePhe: 1.152 ± 0.319
2.216PheGly: 2.216 ± 0.495
0.975PheHis: 0.975 ± 0.244
1.507PheIle: 1.507 ± 0.348
1.507PheLys: 1.507 ± 0.366
2.394PheLeu: 2.394 ± 0.424
0.532PheMet: 0.532 ± 0.166
0.709PheAsn: 0.709 ± 0.208
1.684PhePro: 1.684 ± 0.411
1.33PheGln: 1.33 ± 0.385
1.773PheArg: 1.773 ± 0.433
2.66PheSer: 2.66 ± 0.502
1.596PheThr: 1.596 ± 0.344
0.887PheVal: 0.887 ± 0.277
0.709PheTrp: 0.709 ± 0.257
0.709PheTyr: 0.709 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
8.067GlyAla: 8.067 ± 0.735
0.798GlyCys: 0.798 ± 0.315
4.433GlyAsp: 4.433 ± 0.667
3.901GlyGlu: 3.901 ± 0.599
1.95GlyPhe: 1.95 ± 0.432
7.092GlyGly: 7.092 ± 1.026
1.241GlyHis: 1.241 ± 0.307
4.344GlyIle: 4.344 ± 0.865
3.28GlyLys: 3.28 ± 0.526
7.358GlyLeu: 7.358 ± 0.666
1.507GlyMet: 1.507 ± 0.393
2.305GlyAsn: 2.305 ± 0.421
2.216GlyPro: 2.216 ± 0.39
3.103GlyGln: 3.103 ± 0.581
4.965GlyArg: 4.965 ± 0.552
4.699GlySer: 4.699 ± 1.01
5.23GlyThr: 5.23 ± 0.751
5.23GlyVal: 5.23 ± 0.713
1.507GlyTrp: 1.507 ± 0.371
2.039GlyTyr: 2.039 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
2.216HisAla: 2.216 ± 0.399
0.089HisCys: 0.089 ± 0.089
1.418HisAsp: 1.418 ± 0.327
0.975HisGlu: 0.975 ± 0.242
0.975HisPhe: 0.975 ± 0.279
0.975HisGly: 0.975 ± 0.251
0.443HisHis: 0.443 ± 0.214
1.418HisIle: 1.418 ± 0.355
0.709HisLys: 0.709 ± 0.239
1.596HisLeu: 1.596 ± 0.345
0.266HisMet: 0.266 ± 0.143
0.887HisAsn: 0.887 ± 0.283
0.709HisPro: 0.709 ± 0.296
1.241HisGln: 1.241 ± 0.332
2.128HisArg: 2.128 ± 0.451
1.152HisSer: 1.152 ± 0.335
0.798HisThr: 0.798 ± 0.278
0.887HisVal: 0.887 ± 0.267
0.709HisTrp: 0.709 ± 0.349
0.443HisTyr: 0.443 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
6.294IleAla: 6.294 ± 0.753
0.621IleCys: 0.621 ± 0.283
5.851IleAsp: 5.851 ± 0.584
3.103IleGlu: 3.103 ± 0.593
1.418IlePhe: 1.418 ± 0.387
3.901IleGly: 3.901 ± 0.753
0.887IleHis: 0.887 ± 0.273
2.039IleIle: 2.039 ± 0.568
1.773IleLys: 1.773 ± 0.417
4.699IleLeu: 4.699 ± 0.716
0.975IleMet: 0.975 ± 0.298
1.862IleAsn: 1.862 ± 0.372
2.482IlePro: 2.482 ± 0.698
1.862IleGln: 1.862 ± 0.384
3.369IleArg: 3.369 ± 0.448
3.103IleSer: 3.103 ± 0.652
3.901IleThr: 3.901 ± 0.459
1.773IleVal: 1.773 ± 0.341
0.975IleTrp: 0.975 ± 0.263
0.798IleTyr: 0.798 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
4.965LysAla: 4.965 ± 0.598
0.355LysCys: 0.355 ± 0.193
2.66LysAsp: 2.66 ± 0.576
2.128LysGlu: 2.128 ± 0.528
1.33LysPhe: 1.33 ± 0.369
2.748LysGly: 2.748 ± 0.352
0.621LysHis: 0.621 ± 0.232
1.862LysIle: 1.862 ± 0.53
1.773LysLys: 1.773 ± 0.509
3.812LysLeu: 3.812 ± 0.637
0.798LysMet: 0.798 ± 0.202
1.773LysAsn: 1.773 ± 0.379
2.128LysPro: 2.128 ± 0.384
2.128LysGln: 2.128 ± 0.408
3.989LysArg: 3.989 ± 0.501
1.773LysSer: 1.773 ± 0.334
2.837LysThr: 2.837 ± 0.474
2.394LysVal: 2.394 ± 0.471
0.266LysTrp: 0.266 ± 0.222
1.33LysTyr: 1.33 ± 0.302
0.0LysXaa: 0.0 ± 0.0
Leu
12.411LeuAla: 12.411 ± 1.004
0.887LeuCys: 0.887 ± 0.353
5.585LeuAsp: 5.585 ± 0.549
7.004LeuGlu: 7.004 ± 0.887
3.014LeuPhe: 3.014 ± 0.51
8.156LeuGly: 8.156 ± 1.192
2.482LeuHis: 2.482 ± 0.37
4.965LeuIle: 4.965 ± 0.542
6.826LeuLys: 6.826 ± 0.774
9.929LeuLeu: 9.929 ± 0.939
1.95LeuMet: 1.95 ± 0.433
2.66LeuAsn: 2.66 ± 0.404
4.433LeuPro: 4.433 ± 0.56
5.408LeuGln: 5.408 ± 0.724
7.713LeuArg: 7.713 ± 0.803
6.915LeuSer: 6.915 ± 0.702
6.56LeuThr: 6.56 ± 0.786
5.762LeuVal: 5.762 ± 0.628
1.684LeuTrp: 1.684 ± 0.305
2.394LeuTyr: 2.394 ± 0.445
0.0LeuXaa: 0.0 ± 0.0
Met
3.191MetAla: 3.191 ± 0.556
0.089MetCys: 0.089 ± 0.089
0.975MetAsp: 0.975 ± 0.309
1.064MetGlu: 1.064 ± 0.314
0.355MetPhe: 0.355 ± 0.147
1.684MetGly: 1.684 ± 0.433
0.355MetHis: 0.355 ± 0.162
0.798MetIle: 0.798 ± 0.254
1.064MetLys: 1.064 ± 0.292
2.571MetLeu: 2.571 ± 0.615
0.443MetMet: 0.443 ± 0.22
1.064MetAsn: 1.064 ± 0.296
1.241MetPro: 1.241 ± 0.319
1.241MetGln: 1.241 ± 0.276
1.064MetArg: 1.064 ± 0.387
1.152MetSer: 1.152 ± 0.28
1.152MetThr: 1.152 ± 0.326
0.975MetVal: 0.975 ± 0.303
0.177MetTrp: 0.177 ± 0.112
0.621MetTyr: 0.621 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.191AsnAla: 3.191 ± 0.378
0.443AsnCys: 0.443 ± 0.16
1.596AsnAsp: 1.596 ± 0.329
2.039AsnGlu: 2.039 ± 0.38
1.241AsnPhe: 1.241 ± 0.416
3.901AsnGly: 3.901 ± 0.585
0.887AsnHis: 0.887 ± 0.307
1.241AsnIle: 1.241 ± 0.314
1.152AsnLys: 1.152 ± 0.368
3.723AsnLeu: 3.723 ± 0.52
0.621AsnMet: 0.621 ± 0.207
1.507AsnAsn: 1.507 ± 0.362
1.33AsnPro: 1.33 ± 0.277
1.773AsnGln: 1.773 ± 0.335
1.684AsnArg: 1.684 ± 0.364
1.684AsnSer: 1.684 ± 0.432
1.241AsnThr: 1.241 ± 0.246
1.95AsnVal: 1.95 ± 0.365
0.355AsnTrp: 0.355 ± 0.158
0.709AsnTyr: 0.709 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
5.408ProAla: 5.408 ± 0.477
0.355ProCys: 0.355 ± 0.203
2.305ProAsp: 2.305 ± 0.415
3.635ProGlu: 3.635 ± 0.549
0.887ProPhe: 0.887 ± 0.298
3.457ProGly: 3.457 ± 0.505
0.975ProHis: 0.975 ± 0.274
2.394ProIle: 2.394 ± 0.452
0.975ProLys: 0.975 ± 0.262
5.23ProLeu: 5.23 ± 0.807
1.241ProMet: 1.241 ± 0.288
0.975ProAsn: 0.975 ± 0.391
2.66ProPro: 2.66 ± 0.346
1.95ProGln: 1.95 ± 0.421
3.014ProArg: 3.014 ± 0.681
3.635ProSer: 3.635 ± 0.611
2.571ProThr: 2.571 ± 0.705
2.571ProVal: 2.571 ± 0.384
0.621ProTrp: 0.621 ± 0.204
0.621ProTyr: 0.621 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
7.004GlnAla: 7.004 ± 0.718
0.177GlnCys: 0.177 ± 0.118
1.507GlnAsp: 1.507 ± 0.301
2.305GlnGlu: 2.305 ± 0.467
1.596GlnPhe: 1.596 ± 0.464
3.191GlnGly: 3.191 ± 0.484
0.887GlnHis: 0.887 ± 0.263
2.216GlnIle: 2.216 ± 0.425
2.482GlnLys: 2.482 ± 0.487
5.674GlnLeu: 5.674 ± 0.682
1.862GlnMet: 1.862 ± 0.319
1.507GlnAsn: 1.507 ± 0.35
2.039GlnPro: 2.039 ± 0.451
3.546GlnGln: 3.546 ± 0.658
4.876GlnArg: 4.876 ± 0.686
2.748GlnSer: 2.748 ± 0.427
1.95GlnThr: 1.95 ± 0.36
2.482GlnVal: 2.482 ± 0.372
0.443GlnTrp: 0.443 ± 0.236
1.33GlnTyr: 1.33 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
6.383ArgAla: 6.383 ± 0.838
0.532ArgCys: 0.532 ± 0.172
4.167ArgAsp: 4.167 ± 0.53
4.344ArgGlu: 4.344 ± 0.533
1.95ArgPhe: 1.95 ± 0.393
4.433ArgGly: 4.433 ± 0.715
1.507ArgHis: 1.507 ± 0.359
4.521ArgIle: 4.521 ± 0.766
3.546ArgLys: 3.546 ± 0.51
8.245ArgLeu: 8.245 ± 0.977
1.507ArgMet: 1.507 ± 0.344
2.216ArgAsn: 2.216 ± 0.473
3.191ArgPro: 3.191 ± 0.572
4.787ArgGln: 4.787 ± 0.87
5.23ArgArg: 5.23 ± 0.766
3.546ArgSer: 3.546 ± 0.526
3.103ArgThr: 3.103 ± 0.554
3.635ArgVal: 3.635 ± 0.495
1.418ArgTrp: 1.418 ± 0.344
1.862ArgTyr: 1.862 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
7.801SerAla: 7.801 ± 0.809
0.532SerCys: 0.532 ± 0.197
3.457SerAsp: 3.457 ± 0.504
2.482SerGlu: 2.482 ± 0.441
1.684SerPhe: 1.684 ± 0.542
4.344SerGly: 4.344 ± 0.989
1.596SerHis: 1.596 ± 0.306
2.837SerIle: 2.837 ± 0.528
2.571SerLys: 2.571 ± 0.427
6.206SerLeu: 6.206 ± 0.698
1.507SerMet: 1.507 ± 0.309
1.33SerAsn: 1.33 ± 0.286
3.28SerPro: 3.28 ± 0.376
2.216SerGln: 2.216 ± 0.44
4.078SerArg: 4.078 ± 0.56
3.369SerSer: 3.369 ± 0.709
2.926SerThr: 2.926 ± 0.39
3.723SerVal: 3.723 ± 0.494
0.709SerTrp: 0.709 ± 0.21
1.684SerTyr: 1.684 ± 0.471
0.0SerXaa: 0.0 ± 0.0
Thr
5.94ThrAla: 5.94 ± 0.828
0.709ThrCys: 0.709 ± 0.204
3.014ThrAsp: 3.014 ± 0.451
3.103ThrGlu: 3.103 ± 0.5
2.039ThrPhe: 2.039 ± 0.487
5.762ThrGly: 5.762 ± 0.856
0.443ThrHis: 0.443 ± 0.232
3.546ThrIle: 3.546 ± 0.583
2.039ThrLys: 2.039 ± 0.538
6.649ThrLeu: 6.649 ± 0.617
0.975ThrMet: 0.975 ± 0.303
1.862ThrAsn: 1.862 ± 0.407
2.837ThrPro: 2.837 ± 0.626
1.95ThrGln: 1.95 ± 0.475
3.635ThrArg: 3.635 ± 0.607
2.394ThrSer: 2.394 ± 0.399
3.989ThrThr: 3.989 ± 0.804
3.546ThrVal: 3.546 ± 0.447
1.064ThrTrp: 1.064 ± 0.291
1.684ThrTyr: 1.684 ± 0.425
0.0ThrXaa: 0.0 ± 0.0
Val
6.294ValAla: 6.294 ± 0.98
0.621ValCys: 0.621 ± 0.239
3.191ValAsp: 3.191 ± 0.621
3.723ValGlu: 3.723 ± 0.6
1.684ValPhe: 1.684 ± 0.344
2.748ValGly: 2.748 ± 0.562
0.355ValHis: 0.355 ± 0.157
3.369ValIle: 3.369 ± 0.534
1.95ValLys: 1.95 ± 0.436
5.585ValLeu: 5.585 ± 0.713
1.507ValMet: 1.507 ± 0.362
2.66ValAsn: 2.66 ± 0.544
2.748ValPro: 2.748 ± 0.641
3.103ValGln: 3.103 ± 0.492
3.546ValArg: 3.546 ± 0.464
3.546ValSer: 3.546 ± 0.393
3.812ValThr: 3.812 ± 0.402
3.635ValVal: 3.635 ± 0.577
0.709ValTrp: 0.709 ± 0.232
1.33ValTyr: 1.33 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
2.128TrpAla: 2.128 ± 0.394
0.266TrpCys: 0.266 ± 0.155
1.064TrpAsp: 1.064 ± 0.246
0.709TrpGlu: 0.709 ± 0.202
0.709TrpPhe: 0.709 ± 0.276
0.798TrpGly: 0.798 ± 0.197
0.621TrpHis: 0.621 ± 0.239
0.355TrpIle: 0.355 ± 0.188
0.443TrpLys: 0.443 ± 0.153
2.128TrpLeu: 2.128 ± 0.379
0.177TrpMet: 0.177 ± 0.102
0.266TrpAsn: 0.266 ± 0.15
0.709TrpPro: 0.709 ± 0.237
0.443TrpGln: 0.443 ± 0.197
1.33TrpArg: 1.33 ± 0.358
1.241TrpSer: 1.241 ± 0.337
1.064TrpThr: 1.064 ± 0.327
0.975TrpVal: 0.975 ± 0.345
0.266TrpTrp: 0.266 ± 0.121
0.975TrpTyr: 0.975 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.684TyrAla: 1.684 ± 0.361
0.355TyrCys: 0.355 ± 0.183
2.128TyrAsp: 2.128 ± 0.373
1.95TyrGlu: 1.95 ± 0.408
0.887TyrPhe: 0.887 ± 0.283
1.862TyrGly: 1.862 ± 0.319
0.532TyrHis: 0.532 ± 0.285
1.507TyrIle: 1.507 ± 0.328
0.975TyrLys: 0.975 ± 0.304
3.723TyrLeu: 3.723 ± 0.627
0.532TyrMet: 0.532 ± 0.197
0.532TyrAsn: 0.532 ± 0.216
0.975TyrPro: 0.975 ± 0.339
1.418TyrGln: 1.418 ± 0.469
1.95TyrArg: 1.95 ± 0.306
1.507TyrSer: 1.507 ± 0.388
2.039TyrThr: 2.039 ± 0.486
1.33TyrVal: 1.33 ± 0.355
0.532TyrTrp: 0.532 ± 0.257
0.532TyrTyr: 0.532 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11281 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski