Rhinolophus blasii polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223YUV1|A0A223YUV1_9POLY Minor capsid protein VP2 OS=Rhinolophus blasii polyomavirus 2 OX=2029303 GN=VP3 PE=3 SV=1
MM1 pKa = 7.47GGVLSIILDD10 pKa = 3.84LAEE13 pKa = 4.19ITTDD17 pKa = 3.24LSMSTGASVPLILSGEE33 pKa = 3.83AAAAVEE39 pKa = 4.48AEE41 pKa = 4.46VSSFMLLEE49 pKa = 4.28AMSPLEE55 pKa = 4.1ALAATGITTEE65 pKa = 4.18QFSLLHH71 pKa = 6.12AVPGMVLDD79 pKa = 3.99AVGLGMFFQTFTGASALVAAGLKK102 pKa = 10.33LGLSHH107 pKa = 6.54EE108 pKa = 4.44VSVVNQNMALTIWRR122 pKa = 11.84PEE124 pKa = 4.13EE125 pKa = 3.78YY126 pKa = 9.7WDD128 pKa = 3.52VLFPGVQTFAYY139 pKa = 8.75SLNVLGEE146 pKa = 4.13WATSIYY152 pKa = 10.4HH153 pKa = 6.38AVTRR157 pKa = 11.84QIWDD161 pKa = 3.43SLVRR165 pKa = 11.84EE166 pKa = 4.35GHH168 pKa = 5.1RR169 pKa = 11.84QIGRR173 pKa = 11.84VTSEE177 pKa = 3.5LAYY180 pKa = 10.31RR181 pKa = 11.84SAHH184 pKa = 5.81SFHH187 pKa = 7.02DD188 pKa = 4.22TIARR192 pKa = 11.84IVEE195 pKa = 3.83NARR198 pKa = 11.84WVLTTGPSHH207 pKa = 7.25IYY209 pKa = 9.88SHH211 pKa = 7.37LEE213 pKa = 3.9GYY215 pKa = 10.05YY216 pKa = 10.96SNLPGINPPQARR228 pKa = 11.84ALARR232 pKa = 11.84RR233 pKa = 11.84LQEE236 pKa = 4.67KK237 pKa = 10.37IPDD240 pKa = 3.84RR241 pKa = 11.84YY242 pKa = 8.33TMQRR246 pKa = 11.84EE247 pKa = 4.12SEE249 pKa = 4.29EE250 pKa = 3.99FSGEE254 pKa = 4.19LIEE257 pKa = 5.54HH258 pKa = 6.14VPPPGGAHH266 pKa = 5.71QRR268 pKa = 11.84VTPDD272 pKa = 2.59WLLPLILGLYY282 pKa = 10.46GDD284 pKa = 4.6ITPAWGQQLKK294 pKa = 8.42TLEE297 pKa = 4.19EE298 pKa = 4.4EE299 pKa = 3.91EE300 pKa = 5.19DD301 pKa = 4.0GPKK304 pKa = 10.17KK305 pKa = 9.64KK306 pKa = 10.15RR307 pKa = 11.84KK308 pKa = 8.07RR309 pKa = 11.84RR310 pKa = 11.84RR311 pKa = 11.84MLL313 pKa = 3.61

Molecular weight: 34.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223Z913|A0A223Z913_9POLY Capsid protein VP1 OS=Rhinolophus blasii polyomavirus 2 OX=2029303 GN=VP1 PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 4.89VSPGSSGGTTLTVDD16 pKa = 3.15GMTYY20 pKa = 10.19SVQRR24 pKa = 11.84TFRR27 pKa = 11.84AQTRR31 pKa = 11.84MDD33 pKa = 4.01LQAQALDD40 pKa = 3.39RR41 pKa = 11.84AMDD44 pKa = 4.02MIVDD48 pKa = 4.01SLAVHH53 pKa = 7.01RR54 pKa = 11.84APLQPPLNQKK64 pKa = 7.28SQKK67 pKa = 9.59QVMYY71 pKa = 11.12LMIFLLSFVASLVMLFILIRR91 pKa = 11.84QYY93 pKa = 11.72LPFF96 pKa = 5.99

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1813

96

645

302.2

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.012 ± 0.737

1.986 ± 0.884

4.744 ± 0.454

7.06 ± 0.562

3.806 ± 0.471

6.233 ± 0.79

2.482 ± 0.518

4.633 ± 0.298

6.674 ± 1.515

10.204 ± 0.714

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.365 ± 0.457

4.192 ± 0.949

5.847 ± 0.755

4.026 ± 0.541

4.688 ± 0.924

6.453 ± 0.417

5.571 ± 1.056

6.564 ± 0.713

1.765 ± 0.578

3.696 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski