Lysobacter sp. Alg18-2.2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8KKF6|A0A5C8KKF6_9GAMM DNA gyrase subunit A OS=Lysobacter sp. Alg18-2.2 OX=2603204 GN=gyrA PE=3 SV=1
MM1 pKa = 7.34LAAFVLMCTPALVAAQDD18 pKa = 3.66YY19 pKa = 11.1GNFEE23 pKa = 4.06IGAGFTPDD31 pKa = 3.51PQTGSGVSGGSVDD44 pKa = 3.82ASSFGPGCVGSVDD57 pKa = 4.4DD58 pKa = 5.43SPDD61 pKa = 2.94HH62 pKa = 6.96RR63 pKa = 11.84ITVTSTLDD71 pKa = 3.2LTLYY75 pKa = 10.76ALSSVDD81 pKa = 3.15TTLVLRR87 pKa = 11.84GPAGTFCDD95 pKa = 4.31DD96 pKa = 4.48DD97 pKa = 3.96SHH99 pKa = 8.05GGLNPEE105 pKa = 3.79INARR109 pKa = 11.84LTPGTYY115 pKa = 9.24EE116 pKa = 4.09VYY118 pKa = 10.67VGNFDD123 pKa = 3.93QDD125 pKa = 3.23DD126 pKa = 3.74QARR129 pKa = 11.84YY130 pKa = 7.9TLTLTEE136 pKa = 4.48NLGGTPGADD145 pKa = 3.16EE146 pKa = 4.23EE147 pKa = 4.7SGPRR151 pKa = 11.84TFSLGAGFLPDD162 pKa = 3.49PTIGRR167 pKa = 11.84GITGGDD173 pKa = 2.8MDD175 pKa = 4.86AARR178 pKa = 11.84FGAGCTGKK186 pKa = 9.68VHH188 pKa = 6.52VEE190 pKa = 3.74PDD192 pKa = 3.09HH193 pKa = 6.33VLTVTSAVNLHH204 pKa = 6.43MYY206 pKa = 10.05VDD208 pKa = 4.09SDD210 pKa = 3.37VDD212 pKa = 3.31ATLVVMGPQGAWCDD226 pKa = 3.73DD227 pKa = 3.87DD228 pKa = 6.43SNGNLDD234 pKa = 3.35PAIRR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84FPEE243 pKa = 4.27GVYY246 pKa = 10.09QIYY249 pKa = 10.12IGHH252 pKa = 7.42LGGDD256 pKa = 3.45AGRR259 pKa = 11.84YY260 pKa = 6.39TLTLTEE266 pKa = 4.68DD267 pKa = 3.93LEE269 pKa = 5.07DD270 pKa = 4.08FLL272 pKa = 6.87

Molecular weight: 28.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8KQP2|A0A5C8KQP2_9GAMM TldD/PmbA family protein OS=Lysobacter sp. Alg18-2.2 OX=2603204 GN=FU658_09660 PE=3 SV=1
MM1 pKa = 7.04STDD4 pKa = 3.6SEE6 pKa = 4.41RR7 pKa = 11.84KK8 pKa = 9.82LPIVPIIIIVAAIVLFAIGWWRR30 pKa = 11.84GGARR34 pKa = 11.84PRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84WSSSRR43 pKa = 11.84PSSRR47 pKa = 11.84NRR49 pKa = 11.84VIRR52 pKa = 11.84PPRR55 pKa = 11.84HH56 pKa = 5.39SASATRR62 pKa = 11.84RR63 pKa = 11.84GPAA66 pKa = 3.08

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2448

0

2448

781265

31

1646

319.1

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.476 ± 0.071

0.942 ± 0.017

5.796 ± 0.04

6.029 ± 0.047

3.401 ± 0.033

8.955 ± 0.044

2.411 ± 0.022

3.946 ± 0.035

2.098 ± 0.04

10.922 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.022

2.177 ± 0.027

5.53 ± 0.039

3.435 ± 0.03

8.433 ± 0.057

5.244 ± 0.036

4.545 ± 0.032

7.776 ± 0.04

1.597 ± 0.021

2.01 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski