Cydia pomonella granulosis virus (isolate Mexico/1963) (CpGV) (Cydia pomonella granulovirus)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91EY7|Q91EY7_GVCPM ORF68 P47 OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=orf68 p47 PE=4 SV=1
MM1 pKa = 6.59 TTLLVVIFVMVFVVATVALASGSSSGDD28 pKa = 3.33 VYY30 pKa = 11.02 EE31 pKa = 5.34 GPNDD35 pKa = 4.15 DD36 pKa = 5.88 DD37 pKa = 6.01 VDD39 pKa = 4.87 DD40 pKa = 5.29 EE41 pKa = 4.57 YY42 pKa = 11.62 QSEE45 pKa = 4.44 DD46 pKa = 3.74 EE47 pKa = 4.47 PSEE50 pKa = 4.31 SEE52 pKa = 4.03 PSVSDD57 pKa = 3.63 SVSEE61 pKa = 3.94 YY62 pKa = 10.98 SDD64 pKa = 3.28 VSEE67 pKa = 5.1 YY68 pKa = 11.34 SDD70 pKa = 5.17 DD71 pKa = 5.44 DD72 pKa = 4.09 YY73 pKa = 12.03 LSDD76 pKa = 3.63 HH77 pKa = 7.01 HH78 pKa = 6.63 STSDD82 pKa = 4.04 DD83 pKa = 3.95 SEE85 pKa = 5.8 PDD87 pKa = 3.05 TDD89 pKa = 4.35 LVYY92 pKa = 11.12 SLMYY96 pKa = 9.88 RR97 pKa = 11.84 YY98 pKa = 8.5 PAAIQNEE105 pKa = 5.08 GIAAIHH111 pKa = 6.36 SYY113 pKa = 10.2 IVQQCHH119 pKa = 4.6 QFYY122 pKa = 9.71 PSTPIFPNPFNCEE135 pKa = 3.74 QVFMCMNNNASSNYY149 pKa = 8.05 ATRR152 pKa = 11.84 NCGTSLRR159 pKa = 11.84 GIQWPGGACVLRR171 pKa = 11.84 EE172 pKa = 3.85 EE173 pKa = 4.68 SHH175 pKa = 7.0 CDD177 pKa = 4.25 FYY179 pKa = 11.11 PLPPTT184 pKa = 4.09
Molecular weight: 20.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.88
IPC2_protein 3.681
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.808
Patrickios 0.006
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|Q91EX2|Q91EX2_GVCPM ORF83 similar to AcMNPV ORF103 OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=orf83 PE=4 SV=1
MM1 pKa = 6.67 VRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SRR8 pKa = 11.84 SPNRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 SYY16 pKa = 10.28 RR17 pKa = 11.84 SRR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 SRR25 pKa = 11.84 SRR27 pKa = 11.84 SRR29 pKa = 11.84 SRR31 pKa = 11.84 SRR33 pKa = 11.84 SRR35 pKa = 11.84 SPYY38 pKa = 7.94 RR39 pKa = 11.84 SHH41 pKa = 6.19 YY42 pKa = 10.11 HH43 pKa = 7.05 HH44 pKa = 7.35 INQYY48 pKa = 9.07 II49 pKa = 3.24
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 11.111
IPC_protein 12.31
Toseland 12.442
ProMoST 12.954
Dawson 12.442
Bjellqvist 12.442
Wikipedia 12.925
Rodwell 11.959
Grimsley 12.486
Solomon 12.954
Lehninger 12.852
Nozaki 12.442
DTASelect 12.442
Thurlkill 12.442
EMBOSS 12.954
Sillero 12.442
Patrickios 11.681
IPC_peptide 12.954
IPC2_peptide 11.945
IPC2.peptide.svr19 9.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
143
0
143
36936
48
1131
258.3
29.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.676 ± 0.161
2.54 ± 0.135
6.135 ± 0.124
6.048 ± 0.219
4.44 ± 0.15
4.424 ± 0.174
2.667 ± 0.112
5.442 ± 0.133
6.213 ± 0.232
9.127 ± 0.172
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.683 ± 0.088
6.861 ± 0.163
3.539 ± 0.159
3.568 ± 0.138
5.371 ± 0.16
6.322 ± 0.177
6.154 ± 0.182
8.212 ± 0.202
0.994 ± 0.064
4.586 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here