Pediococcus argentinicus
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1746 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2NHW2|A0A0R2NHW2_9LACO HD superfamily NAD metabolism hydrolase OS=Pediococcus argentinicus OX=480391 GN=IV88_GL000201 PE=4 SV=1
MM1 pKa = 7.95 IYY3 pKa = 8.55 QTFAQLYY10 pKa = 9.9 DD11 pKa = 3.54 EE12 pKa = 5.75 LFDD15 pKa = 4.48 PEE17 pKa = 5.8 EE18 pKa = 3.99 YY19 pKa = 10.72 QNWLDD24 pKa = 3.68 YY25 pKa = 10.92 TNSRR29 pKa = 11.84 VHH31 pKa = 6.28 GKK33 pKa = 7.91 NLKK36 pKa = 9.03 WLEE39 pKa = 4.06 LACGTGHH46 pKa = 8.13 LAINLLKK53 pKa = 10.52 QGQDD57 pKa = 3.1 VTGFDD62 pKa = 4.21 LSEE65 pKa = 4.2 EE66 pKa = 4.09 MLSLAEE72 pKa = 3.88 NHH74 pKa = 6.1 AVEE77 pKa = 4.7 EE78 pKa = 4.68 GVDD81 pKa = 3.75 LPLIQGNMLDD91 pKa = 4.13 LSGLEE96 pKa = 3.93 QYY98 pKa = 10.64 QVVSCYY104 pKa = 10.81 ADD106 pKa = 3.32 SFCYY110 pKa = 10.25 LPSLNDD116 pKa = 3.15 VQGAFQQVAQHH127 pKa = 6.33 LKK129 pKa = 10.48 KK130 pKa = 10.55 DD131 pKa = 3.47 GQFIFDD137 pKa = 4.76 VITPYY142 pKa = 9.17 QTDD145 pKa = 4.11 EE146 pKa = 4.24 IYY148 pKa = 10.3 PGYY151 pKa = 9.16 MFNYY155 pKa = 9.37 QDD157 pKa = 3.69 EE158 pKa = 4.93 DD159 pKa = 4.54 KK160 pKa = 11.32 SFLWSSFGVDD170 pKa = 2.62 EE171 pKa = 4.34 GHH173 pKa = 6.91 RR174 pKa = 11.84 VEE176 pKa = 5.45 HH177 pKa = 7.18 DD178 pKa = 3.15 LTFFIKK184 pKa = 10.62 NKK186 pKa = 10.02 EE187 pKa = 4.08 DD188 pKa = 3.34 MFDD191 pKa = 3.53 KK192 pKa = 10.99 VNEE195 pKa = 4.06 LHH197 pKa = 6.72 TEE199 pKa = 4.05 TTYY202 pKa = 11.42 EE203 pKa = 3.77 LAEE206 pKa = 4.08 YY207 pKa = 10.92 LDD209 pKa = 4.21 ALKK212 pKa = 10.56 QAGFTQIEE220 pKa = 4.53 VTTNFGEE227 pKa = 4.4 STPDD231 pKa = 3.26 DD232 pKa = 3.63 TSTRR236 pKa = 11.84 WFFNCRR242 pKa = 11.84 RR243 pKa = 3.77
Molecular weight: 28.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.932
IPC2_protein 4.215
IPC_protein 4.177
Toseland 3.999
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.037
Rodwell 4.012
Grimsley 3.91
Solomon 4.139
Lehninger 4.101
Nozaki 4.253
DTASelect 4.444
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.291
Patrickios 1.341
IPC_peptide 4.139
IPC2_peptide 4.279
IPC2.peptide.svr19 4.173
Protein with the highest isoelectric point:
>tr|A0A0R2NPV2|A0A0R2NPV2_9LACO Uncharacterized protein OS=Pediococcus argentinicus OX=480391 GN=IV88_GL000959 PE=4 SV=1
MM1 pKa = 7.71 LFPGGGSLMKK11 pKa = 9.8 RR12 pKa = 11.84 TYY14 pKa = 9.69 QPKK17 pKa = 8.69 KK18 pKa = 7.77 RR19 pKa = 11.84 HH20 pKa = 4.99 RR21 pKa = 11.84 QRR23 pKa = 11.84 VHH25 pKa = 6.07 GFRR28 pKa = 11.84 KK29 pKa = 10.03 RR30 pKa = 11.84 MSTSNGRR37 pKa = 11.84 KK38 pKa = 8.01 VLARR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 QKK46 pKa = 10.53 GRR48 pKa = 11.84 RR49 pKa = 11.84 VLSAA53 pKa = 3.76
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1746
0
1746
515383
47
2904
295.2
33.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.024 ± 0.061
0.376 ± 0.012
6.181 ± 0.073
5.932 ± 0.079
4.418 ± 0.045
6.648 ± 0.053
2.043 ± 0.027
7.637 ± 0.061
6.952 ± 0.066
9.417 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.763 ± 0.031
5.387 ± 0.053
3.417 ± 0.033
4.274 ± 0.045
3.857 ± 0.048
6.379 ± 0.05
5.771 ± 0.049
7.202 ± 0.047
0.974 ± 0.02
3.344 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here