Mycobacterium phage Hades

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076YMH1|A0A076YMH1_9CAUD Integrase OS=Mycobacterium phage Hades OX=1527511 GN=43 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 3.91ATVRR6 pKa = 11.84PADD9 pKa = 3.73PVRR12 pKa = 11.84AAIQEE17 pKa = 4.24SLDD20 pKa = 3.61AQGDD24 pKa = 3.98GWQVAHH30 pKa = 5.66YY31 pKa = 9.83VVVAGLEE38 pKa = 4.25RR39 pKa = 11.84ITGDD43 pKa = 4.42RR44 pKa = 11.84MDD46 pKa = 5.24LGATTIITPVGQPDD60 pKa = 4.48YY61 pKa = 10.3LTEE64 pKa = 4.31GLVNRR69 pKa = 11.84YY70 pKa = 7.53WDD72 pKa = 3.72EE73 pKa = 4.72SDD75 pKa = 5.6DD76 pKa = 3.91EE77 pKa = 4.4

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076YRW1|A0A076YRW1_9CAUD Uncharacterized protein OS=Mycobacterium phage Hades OX=1527511 GN=97 PE=4 SV=1
MM1 pKa = 7.41SAFPPPRR8 pKa = 11.84TLTEE12 pKa = 5.0RR13 pKa = 11.84IQGAHH18 pKa = 6.7LNLKK22 pKa = 9.84LARR25 pKa = 11.84QSGNPDD31 pKa = 3.26IIAAAEE37 pKa = 4.34RR38 pKa = 11.84ILNQLVDD45 pKa = 3.93RR46 pKa = 11.84LPRR49 pKa = 11.84STHH52 pKa = 5.09QEE54 pKa = 3.56

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

17644

28

1175

173.0

18.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.576 ± 0.499

1.134 ± 0.168

6.484 ± 0.219

5.775 ± 0.333

2.896 ± 0.177

8.995 ± 0.627

2.222 ± 0.204

4.409 ± 0.249

3.361 ± 0.243

7.107 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.115

3.423 ± 0.177

6.042 ± 0.186

3.503 ± 0.182

6.875 ± 0.401

5.951 ± 0.383

6.875 ± 0.277

7.311 ± 0.283

2.307 ± 0.173

2.414 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski