Tomato leaf curl purple vein virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223HEQ6|A0A223HEQ6_9GEMI Replication enhancer OS=Tomato leaf curl purple vein virus OX=2021667 GN=REn PE=3 SV=1
MM1 pKa = 7.85PPPKK5 pKa = 9.97RR6 pKa = 11.84FRR8 pKa = 11.84ISSKK12 pKa = 10.95NYY14 pKa = 7.93FLTYY18 pKa = 8.78PKK20 pKa = 10.48CSISKK25 pKa = 10.51EE26 pKa = 4.05EE27 pKa = 4.86ALSQLQALEE36 pKa = 4.17TPVNKK41 pKa = 10.18KK42 pKa = 9.89YY43 pKa = 10.65IRR45 pKa = 11.84VCRR48 pKa = 11.84EE49 pKa = 3.31LHH51 pKa = 7.34DD52 pKa = 4.07NGEE55 pKa = 4.25PHH57 pKa = 6.99LHH59 pKa = 6.89ALLQFEE65 pKa = 4.91AKK67 pKa = 9.98FVCTNCRR74 pKa = 11.84FFDD77 pKa = 3.69LRR79 pKa = 11.84HH80 pKa = 6.04PSNSNVCHH88 pKa = 6.55GKK90 pKa = 10.22YY91 pKa = 9.57EE92 pKa = 4.36SCKK95 pKa = 10.3SSSDD99 pKa = 3.0AKK101 pKa = 11.06SYY103 pKa = 10.64IEE105 pKa = 4.17KK106 pKa = 10.82DD107 pKa = 2.78GDD109 pKa = 3.82YY110 pKa = 11.34VEE112 pKa = 5.19WGDD115 pKa = 3.63FQIDD119 pKa = 3.22GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.24TYY134 pKa = 11.73AKK136 pKa = 10.46ALNASSAEE144 pKa = 4.0EE145 pKa = 3.51ALQIIKK151 pKa = 10.28EE152 pKa = 4.13EE153 pKa = 4.13QPQHH157 pKa = 6.73FFLQHH162 pKa = 6.07HH163 pKa = 6.14NLVANAQRR171 pKa = 11.84IFQKK175 pKa = 10.72APEE178 pKa = 4.1PWAPPFPLSSFTNVPEE194 pKa = 4.81EE195 pKa = 4.01MQEE198 pKa = 3.86WADD201 pKa = 3.96DD202 pKa = 3.84YY203 pKa = 11.51LGRR206 pKa = 11.84DD207 pKa = 3.04AAARR211 pKa = 11.84PEE213 pKa = 4.08RR214 pKa = 11.84PISIIIEE221 pKa = 4.2GDD223 pKa = 3.24SRR225 pKa = 11.84TGKK228 pKa = 8.52TMWARR233 pKa = 11.84SLGSHH238 pKa = 6.68NYY240 pKa = 10.27LSGHH244 pKa = 6.92LDD246 pKa = 3.48FNSRR250 pKa = 11.84VYY252 pKa = 11.45SNDD255 pKa = 2.81AEE257 pKa = 4.47YY258 pKa = 11.31NVIDD262 pKa = 5.1DD263 pKa = 4.01VTPQYY268 pKa = 11.68LKK270 pKa = 9.62MKK272 pKa = 9.18HH273 pKa = 4.39WKK275 pKa = 9.54EE276 pKa = 3.85LIGAQRR282 pKa = 11.84DD283 pKa = 3.53WQSNCKK289 pKa = 9.41YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.21GSSYY314 pKa = 11.34KK315 pKa = 10.75DD316 pKa = 3.2FLEE319 pKa = 4.2KK320 pKa = 10.55QEE322 pKa = 4.15NVSLKK327 pKa = 10.51NWTLHH332 pKa = 4.51NAKK335 pKa = 10.29FIFLDD340 pKa = 3.54SPLYY344 pKa = 10.2QSPSQGGQEE353 pKa = 4.17EE354 pKa = 4.62GDD356 pKa = 3.41

Molecular weight: 40.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223HEP8|A0A223HEP8_9GEMI Replication-associated protein OS=Tomato leaf curl purple vein virus OX=2021667 GN=Rep PE=3 SV=1
MM1 pKa = 7.68SKK3 pKa = 10.29RR4 pKa = 11.84AAPWRR9 pKa = 11.84SFAGMSKK16 pKa = 8.86VQRR19 pKa = 11.84SLNFSPRR26 pKa = 11.84SGTGPKK32 pKa = 9.81LDD34 pKa = 4.6SRR36 pKa = 11.84SAWVNRR42 pKa = 11.84PMYY45 pKa = 9.63RR46 pKa = 11.84KK47 pKa = 8.95PRR49 pKa = 11.84IYY51 pKa = 9.54RR52 pKa = 11.84TLRR55 pKa = 11.84SPDD58 pKa = 3.35VPKK61 pKa = 10.93GCEE64 pKa = 4.39GPCKK68 pKa = 9.35VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.24ILGKK114 pKa = 9.73VWMDD118 pKa = 3.29EE119 pKa = 4.01NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.85NDD166 pKa = 2.82LRR168 pKa = 11.84DD169 pKa = 3.59RR170 pKa = 11.84FQVLHH175 pKa = 6.48KK176 pKa = 10.11FYY178 pKa = 11.3SKK180 pKa = 9.77VTGGQYY186 pKa = 11.06ASNEE190 pKa = 3.84QALVRR195 pKa = 11.84RR196 pKa = 11.84FWRR199 pKa = 11.84VNTHH203 pKa = 3.46VTYY206 pKa = 11.01NHH208 pKa = 5.92QEE210 pKa = 3.49AAKK213 pKa = 10.4YY214 pKa = 9.25EE215 pKa = 4.13NHH217 pKa = 6.46TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.59FYY246 pKa = 11.32DD247 pKa = 4.06SISNN251 pKa = 3.66

Molecular weight: 29.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1046

87

356

174.3

19.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.067 ± 0.776

2.008 ± 0.35

4.589 ± 0.484

4.876 ± 0.969

4.015 ± 0.564

5.545 ± 0.704

4.493 ± 0.764

5.641 ± 0.903

5.258 ± 0.963

8.031 ± 1.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.713

6.023 ± 0.491

6.023 ± 0.669

4.589 ± 0.715

6.501 ± 1.023

8.413 ± 0.567

5.258 ± 1.034

6.31 ± 1.292

1.53 ± 0.302

3.728 ± 0.771
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski