Ochrobactrum phage POI1126
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A240F4V3|A0A240F4V3_9CAUD Uncharacterized protein OS=Ochrobactrum phage POI1126 OX=1932118 GN=POI1126_55 PE=4 SV=1
MM1 pKa = 6.98 STSEE5 pKa = 4.56 HH6 pKa = 5.8 KK7 pKa = 10.6 RR8 pKa = 11.84 VWLPEE13 pKa = 3.82 EE14 pKa = 4.26 FADD17 pKa = 4.91 EE18 pKa = 3.89 ISPRR22 pKa = 11.84 SLEE25 pKa = 4.13 SPDD28 pKa = 3.88 DD29 pKa = 4.12 FIRR32 pKa = 11.84 RR33 pKa = 11.84 TDD35 pKa = 3.08 EE36 pKa = 4.19 GNFVLSARR44 pKa = 11.84 DD45 pKa = 3.28 EE46 pKa = 4.58 DD47 pKa = 5.03 DD48 pKa = 4.33 NFILEE53 pKa = 4.09 GDD55 pKa = 3.91 FTFSVPLAQGDD66 pKa = 4.17 VVVFWPYY73 pKa = 10.43 EE74 pKa = 3.88 DD75 pKa = 4.32 YY76 pKa = 11.37 GAFSVWVDD84 pKa = 2.82 ADD86 pKa = 3.47 GKK88 pKa = 10.7 YY89 pKa = 8.6 RR90 pKa = 11.84 TDD92 pKa = 3.06 AFFPALANNFYY103 pKa = 11.22 VQGDD107 pKa = 3.2 WDD109 pKa = 3.87 AYY111 pKa = 8.67 GTFGEE116 pKa = 5.06 LIEE119 pKa = 4.82 SDD121 pKa = 3.93 SFADD125 pKa = 4.49 ALKK128 pKa = 10.5 SDD130 pKa = 4.87 PKK132 pKa = 9.85 PTICIGQSFPGVSFRR147 pKa = 11.84 FDD149 pKa = 3.09 IDD151 pKa = 3.61 SDD153 pKa = 3.93 GNPRR157 pKa = 11.84 FVRR160 pKa = 11.84 QAWVNN165 pKa = 3.47
Molecular weight: 18.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.247
IPC2_protein 3.935
IPC_protein 3.935
Toseland 3.719
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.757
Grimsley 3.63
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.291
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.062
Patrickios 3.706
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.984
Protein with the highest isoelectric point:
>tr|A0A240F4T2|A0A240F4T2_9CAUD Uncharacterized protein OS=Ochrobactrum phage POI1126 OX=1932118 GN=POI1126_29 PE=4 SV=1
MM1 pKa = 7.81 PLHH4 pKa = 6.78 LKK6 pKa = 8.94 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 8.36 YY10 pKa = 10.95 KK11 pKa = 10.38 LFRR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 9.52 KK17 pKa = 10.42 RR18 pKa = 11.84 LFGLNGYY25 pKa = 7.8 WCYY28 pKa = 9.91 PAGSDD33 pKa = 3.7 KK34 pKa = 10.93 RR35 pKa = 11.84 VIAVRR40 pKa = 11.84 LDD42 pKa = 3.65 RR43 pKa = 11.84 LCDD46 pKa = 3.73 KK47 pKa = 10.99 AWARR51 pKa = 11.84 CRR53 pKa = 11.84 DD54 pKa = 3.49 AAIEE58 pKa = 4.02 RR59 pKa = 11.84 AVGLGG64 pKa = 3.21
Molecular weight: 7.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.838
IPC_protein 10.643
Toseland 10.687
ProMoST 10.409
Dawson 10.804
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.038
Grimsley 10.862
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.789
IPC_peptide 10.906
IPC2_peptide 9.692
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
18435
37
4841
236.3
26.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.901 ± 0.375
0.705 ± 0.215
6.607 ± 0.291
6.547 ± 0.239
3.358 ± 0.141
7.274 ± 0.393
1.795 ± 0.177
5.435 ± 0.258
5.256 ± 0.259
7.551 ± 0.311
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.087
3.83 ± 0.171
4.991 ± 0.202
4.188 ± 0.241
7.231 ± 0.248
5.495 ± 0.174
5.381 ± 0.197
5.788 ± 0.203
1.644 ± 0.188
2.604 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here